A5101598032- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- Mosquito-borne diseases and control
- Blood properties and coagulation
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- Complement system in diseases
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- DNA Repair Mechanisms
- Kruppel-like factors research
- Ubiquitin and proteasome pathways
- Signaling Pathways in Disease
- Cancer, Lipids, and Metabolism
Inserm
2016-2025
Institut de Cancérologie de l'Ouest
2022-2025
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2022-2025
Centre National de la Recherche Scientifique
2016-2025
Université d'Angers
2024-2025
Nantes Université
2023-2025
Université Paris Cité
2015-2024
Institut Cochin
2014-2024
University of Liège
2006-2023
Hôpital Cochin
2023
Human mesenchymal stem/stromal cells (hMSCs) have been shown to support breast cancer cell proliferation and metastasis, partly through their secretome. hMSCs a remarkable ability survive for long periods under stress, secretome is tumor supportive. In this study, we characterized the cargo of extracellular vesicular (EV) fraction (that in size range 40-150nm) serum deprived (SD-MSCs). Next Generation Sequencing assays were used identify small RNA secreted EVs, which indicated presence...
Mass spectrometry-based proteomics now enables the absolute quantification of thousands proteins in individual cell types. We used this technology to analyze dynamic proteome changes occurring during human erythropoiesis. quantified expression 6,130 erythroid differentiation from late burst-forming units-erythroid (BFU-Es) orthochromatic erythroblasts. A modest correlation between mRNA and protein was observed. identified several with unexpected patterns cells, highlighting a breakpoint...
The role of insect saliva in the first contact between an and a plant is crucial during feeding. Some elicitors, particularly regurgitants, have been identified as inducing defence reactions. Here, we focused on salivary proteome green peach aphid, Myzus persicae. Proteins were either directly in-solution digested or separated by 2D SDS-PAGE before trypsin digestion. Resulting peptides then mass spectrometry coupled with database investigations. A homemade was constituted expressed sequence...
β-catenin signaling can be both a physiological and oncogenic pathway in the liver. It controls compartmentalized gene expression, allowing liver to ensure its essential metabolic function. is activated by mutations 20%-40% of hepatocellular carcinomas (HCCs) with specific features. We decipher molecular determinants β-catenin-dependent zonal transcription using mice β-catenin-activated or -inactivated hepatocytes, characterizing vivo their chromatin occupancy T-cell factor (Tcf)−4...
Significance Human immunodeficiency viruses (HIV) have developed strategies to interfere with DNA repair in host cells. Some pathways represent restriction mechanisms that counteract the virus as soon it penetrates into cell, before establishment of an interferon response. Here we identify helicase-like transcription factor (HLTF) a new protein degraded by viral R (Vpr) from HIV-1. HLTF mediates stalled replication forks bypass lesions and ensure genome integrity. is early after Vpr delivery...
Abstract RNA G-quadruplexes (RG4s) are four-stranded structures known to control mRNA translation of cancer relevant genes. RG4 formation is pervasive in vitro but not cellulo, indicating the existence poorly characterized molecular machinery that remodels RG4s and maintains them unfolded. Here, we performed a quantitative proteomic screen identify cytosolic proteins interact with canonical its folded unfolded conformation. Our results identified hnRNP H/F as important components cytoplasmic...
Glioblastoma are highly aggressive brain tumours that associated with an extremely poor prognosis. Within these exists a subpopulation of plastic self-renewing cancer cells retain the ability to expand ex vivo as tumourspheres, induce tumour growth in mice, and have been implicated radio- chemo-resistance. Although their identity fate regulated by external cues emanating from endothelial cells, nature such signals remains unknown. Here, we used mass spectrometry proteomic approach...
A variant erythroferrone contributes to hepcidin modulation and systemic iron accumulation in patients with SF3B1 -mutated myelodysplastic syndrome.
Abstract The Wnt/β‐catenin signaling pathway has been increasingly implicated in liver development and physiology. Aberrant activation of this is one the major genetic events observed during process human HCC development. To gain insight into mechanism underlying β‐catenin action liver, we conducted a quantitative differential proteomic analysis using 2‐D DIGE combined with MS, mice liver‐specific deletion Apc resulting acute (Apc KOliv mice). We identified 94 protein spots showing...
The blood—brain barrier (BBB) selectively controls the exchanges between blood and brain: it is formed by tight junctions (TJs) adjacent microvascular endothelial cells. transmembrane protein claudin-5 known as a key TJ at BBB, although, molecular mechanisms which regulates tightness are poorly understood. To identify putative partners that contribute to integrity, claudin-5-enriched membrane microdomains were prepared cell fractionation, using human brain line hCMEC/D3 immunoprecipitates...
Background Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is a highly polymorphic adherence receptor expressed on the surface of infected erythrocytes. Based sequence homology PfEMP-1 variants have been grouped into three major groups A-C, conserved VAR2CSA variants, and semi-conserved types defined by tandem runs specific domains ("domain cassettes" (DC)). The type determines phenotype, associated with clinical outcome infection. Methods Parasite isolates from Beninese...
Abstract Objectives Venetoclax combined with hypomethylating agents is a new therapeutic strategy frequently used for treating AML patients who are not eligible conventional chemotherapy. However, high response rates heterogeneous due to different mechanisms mediating resistance venetoclax such as up‐regulation of MCL‐1 expression. We thus tested the anti‐leukemic activity S63845, specific inhibitor. Methods Apoptosis induces by S63845 or without was evaluated in primary samples and cell...
Takotsubo cardiomyopathy is a stress-induced cardiovascular disease with symptoms comparable to those of an acute coronary syndrome but without obstruction. was initially considered spontaneously reversible, epidemiological studies revealed significant long-term morbidity and mortality, the reason for which unknown. Here, we show in female rodent model that single pharmacological challenge creates similar Takotsubo. The response involves changes blood tissue biomarkers cardiac vivo imaging...
Most of the recent developments aiming to coupling between surface plasmon resonance (SPR) and mass spectrometry (MS) are based on use a biochip with limited number flow cells requiring elution steps for recovery captured biomolecules. In this work, direct on-chip MALDI-MS detection is presented using SPRi-sensor in microarray format that allows multiplex SPR−MS analysis. The gold was functionalized by self-assembled monolayer (SAM) short polyoxyethylene (POE) chains carrying...
p27Kip1 (p27) is a cyclin-CDK inhibitor and negative regulator of cell proliferation. p27 also controls other cellular processes including migration cytoplasmic can act as an oncogene. Furthermore, promotes invasion metastasis, in part by promoting epithelial to mesenchymal transition. Herein, we find that binding regulating the activity Cortactin, critical invadopodia formation. localizes limits their number activity. interaction Cortactin with PAK1. In turn, PAK1 turnover phosphorylating...
Protozoan pathogens secrete nanosized particles called extracellular vesicles (EVs) to facilitate their survival and chronic infection. Here, we show the inhibition by Plasmodium berghei NK65 blood stage-derived EVs of proliferative response CD4+ T cells in antigen presentation. Importantly, these results were confirmed vivo capacity diminish ovalbumin-specific delayed type hypersensitivity response. We identified two proteins associated with EVs, histamine releasing factor (HRF) elongation...
Abstract Fas-associated death domain (FADD) is a key adaptor molecule involved in numerous physiological processes including cell death, proliferation, innate immunity and inflammation. Therefore, changes FADD expression have dramatic cellular consequences. In mice humans, regulation can occur through protein secretion. However, the molecular mechanisms accounting for human secretion were still unknown. Here we report that canonical, non-canonical, but not alternative, NLRP3 inflammasome...
Abstract Murine-based cellular models have provided and continue to provide many useful insights into the fundamental mechanisms of erythropoiesis, as well pathophysiology inherited acquired red cell disorders. Although detailed information on aspects these is available, comprehensive proteomic data are lacking. This a critical knowledge gap, proteins effectors most biologic processes. To address this unmet need, proteomes murine lines Friend erythroleukemia (MEL), GATA1 erythroid (G1ER),...
Abstract Excessive glucose production by the liver is a key factor in hyperglycemia observed type 2 diabetes mellitus (T2DM). Here, we highlight novel role of kinase B1 (Lkb1) this regulation. We show that mice with hepatocyte-specific deletion Lkb1 have higher levels hepatic amino acid catabolism, driving gluconeogenesis. This effect during both fasting and postprandial period, identifying as critical suppressor Hepatic associated major changes whole-body metabolism, leading to lower lean...