A5063451838- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Curcumin's Biomedical Applications
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Cancer, Lipids, and Metabolism
- Cancer therapeutics and mechanisms
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Hormonal Regulation and Hypertension
- Peroxisome Proliferator-Activated Receptors
- Computational Drug Discovery Methods
- Synthesis and Characterization of Heterocyclic Compounds
- Osteoarthritis Treatment and Mechanisms
- Epigenetics and DNA Methylation
University of Mississippi Medical Center
2013-2024
University of Mississippi
2013-2023
Damietta University
2021
Praxis (United States)
2021
Prompt (Canada)
2021
Jackson Memorial Hospital
2016-2017
Gene Therapy Laboratory
2008-2011
Tulane University
2007-2011
Université Laval
2004-2008
Inserm
2000-2001
Human mesenchymal stem/stromal cells (hMSCs) have been shown to support breast cancer cell proliferation and metastasis, partly through their secretome. hMSCs a remarkable ability survive for long periods under stress, secretome is tumor supportive. In this study, we characterized the cargo of extracellular vesicular (EV) fraction (that in size range 40-150nm) serum deprived (SD-MSCs). Next Generation Sequencing assays were used identify small RNA secreted EVs, which indicated presence...
We observed that microRNAs (miRNAs) regulate differentiation in a variety of simpler systems also human multipotent stromal cells (hMSCs) from bone marrow. Differentiation hMSCs into osteoblasts and adipocytes was inhibited by using lentiviruses expressing shRNAs to decrease expression Dicer Drosha, two enzymes process early transcripts miRNA. Expression analysis miRNAs during hMSC identified 19 were up-regulated osteogenic 20 adipogenic differentiation, 11 which commonly both...
Recent studies have implicated multipotential mesenchymal stem cells (MSCs) as an aid to breast cancer cell proliferation and metastasis, partly a result of the MSCs secretome. As tumor gets beyond 2 mm in diameter, stromal could undergo starvation due lack sufficient nutrients solid microenvironment. In this study, we investigated survival mechanisms used by stressed cancers. We serum-deprived (SD-MSCs) MCF-7 model system with hypothesis that nutrient-deprived core utilize for supporting...
This study examines the antitumor potential of curcumin and C6 ceramide (C6) against osteosarcoma (OS) cell lines when both are encapsulated in bilayer liposomal nanoparticles. Three formulations were prepared: liposomes, liposomes C6-curcumin liposomes. Curcumin combination with showed 1.5 times enhanced cytotoxic effect case MG-63 KHOS OS lines, comparison alone. Importantly, found to be less toxic on untransformed primary human cells (human mesenchymal stem cells) lines. In addition,...
Uncoupling proteins (UCP), specific mitochondrial proton transporters that function by uncoupling oxidative metabolism from ATP synthesis, are involved in thermoregulation and control of energy expenditure. The hibernoma-derived T37i cells, which possess functional endogenous mineralocorticoid receptors (MR), can undergo differentiation into brown adipocytes. In differentiated UCP1 mRNA levels increased 10- to 20-fold after retinoic acid or β-adrenergic treatment. Interestingly, UCP2 UCP3...
OBJECTIVE—Synthetic ligands for peroxisome proliferator–activated receptor-γ (PPAR-γ) improve insulin sensitivity in obesity, but it is still unclear whether inflammatory signals modulate their metabolic actions. In this study, we tested targeted disruption of inducible nitric oxide (NO) synthase (iNOS), a key mediator modulates the effects rosiglitazone obese mice. RESEARCH DESIGN AND METHODS—iNOS−/− and iNOS+/+ were subjected to high-fat diet or standard 18 weeks then treated with 2 weeks....
By use of targeted oncogenesis, a brown adipocyte cell line was derived from hibernoma transgenic mouse carrying the proximal promoter human mineralocorticoid receptor (MR) linked to SV40 large T antigen. T37i cells remain capable differentiating into adipocytes upon insulin and triiodothyronine treatment as judged by their ability express uncoupling protein 1 maintain MR expression. Aldosterone undifferentiated induced accumulation intracytoplasmic lipid droplets mitochondria. This effect...
// Krishna C. Vallabhaneni 1, 2 , Patrice Penfornis 1 Fei Xing 3 Yoni Hassler Kristen V. Adams 4 Yin-Yuan Mo Kounosuke Watabe 3, 5 and Radhika Pochampally 6 Cancer Institute, University of Mississippi Medical Center, Jackson, MS 39216, USA Department Radiation Oncology, Biology, Wake Forest School Medicine, Winston-Salem, NC 27157, Pathology, Comprehensive Biochemistry, Correspondence to: Pochampally, email: rpochampally@umc.edu Keywords: exosomes; dormancy; MSCs; miRNA; signalling Received:...
The human mineralocorticoid receptor (hMR) is a nuclear mediating aldosterone action, whose expression driven by two alternative promoters, P1 and P2, flanking the first 5'-untranslated exons. In vivo characterization of hMR regulatory regions was performed targeted oncogenesis in mice using or P2 directing large T antigen SV40 (TAg). While transgenic P1.TAg founders rapidly developed lethal hibernomas from brown fat, cerebral primitive neuroectodermal tumors facial leiomyosarcomas occurred...
The role of inducible nitric oxide synthase (iNOS) in the modulation adipocyte lipolysis was investigated. Treatment white and brown adipose cell lines mouse explants with a mixture tumor necrosis factor-α, interferon-γ, lipopolysaccharide (LPS) doubled lipolytic rate, this associated marked induction iNOS expression (NO) production. inhibition by 1400W, aminoguanidine, or L-NIL pretreatment further increased cytokine/LPS-mediated 30% (P < 0.05) cultured adipocytes explants. However,...
Background: Extra-axial chordomas are rare low-grade malignant tumors thought to arise from notochordal remnants in the extra-axial skeleton. Few studies have been done on this neoplasm because of its rarity. In addition, there is a lack good vitro model which perform more characterization. Methods: We describe twenty-eight-year-old man with mass right scapula. Cytomorphology and immunohistochemistry, including brachyury staining, were used formulate final diagnosis. A fragment tumor was...
In this study, we used multipotential MSCs and microarray assays to follow the changing patterns of gene expression as were differentiated osteoblasts. We analyzed co-expressed groups identify new targets for known transcription factor VDR during differentiation. The roles two genes (histamine receptor H1 dermatopontin) downstream confirmed by gel electromotility shift, siRNA inhibition, chromatin immunoprecipitation assays.Osteogenesis is stringently controlled osteoblast-specific signaling...
Since aerobic glycolysis was first observed in tumors almost a century ago by Otto Warburg, the field of cancer cell metabolism has sparked interest scientists around world as it might offer new avenues treatment for malignant cells. Our current study claims discovery gnetin H (GH) novel inhibitor that can decrease metabolic activity and lactic acid synthesis displays strong cytostatic effect melanoma glioblastoma Compared to most other inhibitors used combination with complex-1...
Overall prognosis for osteosarcoma (OS) is poor despite aggressive treatment options. Limited access to primary tumors, technical challenges in processing OS tissues, and the lack of well-characterized cell cultures has hindered our ability fully understand properties tumor initiation progression. In this study, we have isolated characterized derived from four central high-grade human samples. Furthermore, used study role CD49f Recent studies implicated stemness multipotency both cancer stem...