A5054702207- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Toxoplasma gondii Research Studies
- Reconstructive Surgery and Microvascular Techniques
- Genetic Syndromes and Imprinting
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Mycobacterium research and diagnosis
- Cervical Cancer and HPV Research
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Estrogen and related hormone effects
- Viral-associated cancers and disorders
- T-cell and Retrovirus Studies
- Orthopaedic implants and arthroplasty
- Radiomics and Machine Learning in Medical Imaging
- Traumatic Brain Injury and Neurovascular Disturbances
- Connective tissue disorders research
Tulane University
2015-2024
University of Sheffield
2024
Sheffield Hallam University
2023
Trinity College Dublin
2019
University of New Orleans
2013-2014
Western University
2014
University of Florida
2014
Loyola Medicine
2014
Louisiana Cancer Research Center
2013
University Medical Center New Orleans
2013
Abstract In regenerative medicine, bone marrow is a promising source of mesenchymal stem cells (MSCs) for broad range cellular therapies. This research addresses basic prerequisite to realize the therapeutic potential MSCs by developing novel high-capacity assay quantify clonal heterogeneity in potency that inherent MSC preparations. The utilizes 96-well format (1) classify according colony-forming efficiency as measure proliferation capacity and trilineage exhibit adipo-, chondro-,...
ABSTRACT The Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is a functional homologue of the tumor necrosis factor receptor family and contributes substantially to oncogenic potential EBV through activation nuclear κB (NF-κB). MicroRNAs (miRNAs) are class small RNA molecules that involved in regulation cellular processes such as growth, development, apoptosis have recently been linked cancer phenotypes. Through miRNA microarray analysis, we demonstrate LMP1 dysregulates...
Structural genetic alterations in cancer often involve gene loss or amplification. With the advent of microarray approaches for analysis genome, as exemplified by array–CGH ( C omparative G enomic H ybridization), scanning gene-dosage is limited only issues DNA density. However, samples interest to pathologist comprise small clusters just a few hundred cells, which do not provide sufficient analysis. We sought develop simple method that would permit amplification whole genome without use...
The cellular microRNA miR-155 has been shown to be involved in lymphocyte activation and is expressed Epstein-Barr virus (EBV)-infected cells displaying type III latency gene expression but not I expression. We show here that the elevated levels of due EBV epigenetic differences cell lines tested, we EBV-infected requires a conserved AP-1 element promoter. Gene analysis was carried out line transduced with an miR-155-expressing retrovirus. This identified both miR-155-suppressed -induced...
Background. Tuberculosis (TB) leads to the death of 1.7 million people annually. The failure bacille Calmette-Guérin vaccine, synergy between AIDS and TB, emergence drug resistance have worsened this situation. It is imperative delineate mechanisms employed by Mycobacterium tuberculosis successfully infect persist in mammalian lungs.
Epstein-Barr virus (EBV) is associated with roughly 10% of gastric carcinomas worldwide (EBVaGC). Although previous investigations provide a strong link between EBV and carcinomas, these studies were performed using selected gene probes. Using cohort carcinoma RNA-seq data sets from The Cancer Genome Atlas (TCGA), we quantitative global assessment expression in assessed cellular pathway alterations. transcripts detected 17% samples but varied significantly coverage depth. In four the highest...
Myogenic cell cultures derived from muscle biopsies are excellent models for human differentiation. We report the first comprehensive analysis of myogenesis-specific DNA hyper- and hypo-methylation throughout genome progenitor cells (both myoblasts myotubes) skeletal tissue vs. 30 non-muscle samples using reduced representation bisulfite sequencing. also focused on four genes with extensive or in lineage (PAX3, TBX1, MYH7B/MIR499 OBSCN) to compare methylation, DNaseI hypersensitivity,...
Abstract Background Facioscapulohumeral muscular dystrophy (FSHD) is a dominant disease linked to contraction of an array tandem 3.3-kb repeats (D4Z4) at 4q35. Within each repeat unit gene, DUX4 , that can encode protein containing two homeodomains. A transcript derived from the last in contracted associated with pathogenesis but it unclear how. Methods Using exon-based microarrays, expression profiles myogenic precursor cells were determined. Both undifferentiated myoblasts and...
SOX9, a high mobility group (HMG) box transcription factor, is required for development, differentiation and lineage commitment. It known to exert its effects through nuclear translocation, such as cell cycle changes in response retinoic acid treatment breast cancer cells. However, it not whether SOX9 has prognostic significance human cancer. Over-expression cytoplasmic sequestration of proteins are implicated tumor progression. To determine any cancer, expression subcellular localization...
Abstract Rapid assessment of prostate core biopsy pathology at the point-of-procedure could provide benefit in a variety clinical situations. Even with advanced transrectal ultrasound guidance and saturation protocols, cancer can be missed up to half all initial procedures. In addition, collection tumor specimens for downstream histologic, molecular, genetic analysis is hindered by low yield due inability identify grossly. However, current such as frozen section (FSA), are destructive too...
Differentially methylated or hydroxymethylated regions (DMRs) in mammalian DNA are often associated with tissue-specific gene expression but the functional relationships still being unraveled. To elucidate these relationships, we studied 16 human genes containing myogenic DMRs by analyzing profiles of their epigenetics and transcription quantitatively assaying 5-hydroxymethylcytosine (5hmC) 5-methylcytosine (5mC) at specific sites skeletal muscle (SkM), myoblasts, heart, brain, diverse other...
Bioinformatics and data science research has boundless potential across Africa due to its high levels of genetic diversity disproportionate burden infectious diseases, including malaria, tuberculosis, HIV/AIDS, Ebola virus disease, Lassa fever. This work lays out an incremental training approach for engaging underserved populations in bioinformatics at the University Sciences, Techniques Technologies Bamako, Mali (USTTB). Two informatics programs sponsored by Fogarty International Center...
The northeast Nile Delta region exhibits a high incidence of early-onset pancreatic cancer. It is well documented that this has one the highest levels pollution in Egypt. Epidemiologic studies have suggested cadmium, prevalent pollutant region, plays role development cancer.Objective: We aimed to assess serum cadmium as markers exposure cancer patients and noncancer comparison subjects from same Egypt.Design Participants: assessed 31 newly diagnosed 52 hospital Mansoura,...
In Brief BACKGROUND Increased intracranial pressure (ICP) can cause brain ischemia and compromised oxygen (PbtO2 ≤ 20 mm Hg) after severe traumatic injury (TBI). OBJECTIVE We examined whether decompressive craniectomy (DC) to treat elevated ICP reduces the cumulative ischemic burden (CIB) of therapeutic intensity level (TIL). METHODS Ten TBI patients (mean age, 31.4 ± 14.2 years) who had continuous PbtO2 monitoring before delayed DC were retrospectively identified. Patients managed according...
Glyceollins, a group of novel phytoalexins isolated from activated soy, have recently been demonstrated to be antiestrogens that bind the estrogen receptor (ER) and inhibit estrogen-induced tumor progression. Our previous publications focused specifically on inhibition formation growth by glyceollin mixture, which contains three isomers (I, II, III). Here, we show mixture is also effective as potential antiestrogenic, therapeutic agent prevents estrogen-stimulated tumorigenesis displays...
Human mesenchymal stem cells (MSCs) from bone marrow are a heterogeneous ensemble of progenitors and lineage-committed cells, with broad range regenerative properties. Ex vivo expansion to produce sufficient quantities MSCs is essential for most therapeutic applications. The present study resolves the relationship between proliferation potential their potency. Clonal analysis generated single-cell derived colonies that were classified according trilineage exhibit adipo- (A), chondro- (C),...
Abstract Background Tight regulation of homeobox genes is essential for vertebrate development. In a study genome-wide differential methylation, we recently found that genes, including those in the HOX gene clusters, were highly overrepresented among with hypermethylation skeletal muscle lineage. Methylation was analyzed by reduced representation bisulfite sequencing (RRBS) postnatal myoblasts, myotubes and adult tissue 30 types non-muscle-cell cultures or tissues. Results this study,...
Notch intercellular signaling is critical for diverse developmental pathways and homeostasis in various types of stem cells progenitor cells. Because gene products need to be precisely regulated spatially temporally, epigenetics likely help control expression genes. Reduced representation bisulfite sequencing (RRBS) indicated significant hypomethylation myoblasts, myotubes, skeletal muscle vs. many nonmuscle samples at intragenic or intergenic regions the following receptor ligand genes:...
Cytosine modifications in DNA such as 5-methylcytosine (5mC) underlie a broad range of developmental processes, maintain cellular lineage specification, and can define or stratify types cancer other diseases. However, the wide variety approaches available to interrogate these has created need for harmonized materials, methods, rigorous benchmarking improve genome-wide methylome sequencing applications clinical basic research. Here, we present multi-platform assessment cross-validated...
De novo limb regeneration after amputation is restricted in mammals to the distal digit tip. Central this regenerative process blastema, a heterogeneous population of lineage-restricted, dedifferentiated cells that ultimately orchestrates amputated bone and surrounding soft tissue. To investigate skeletal regeneration, we made use spatial transcriptomics characterize transcriptional profile specifically within blastema. Using technique, generated gene signature with high specificity for...
While studying myoblast methylomes and transcriptomes, we found that CDH15 had a remarkable preference for expression in both myoblasts cerebellum. To understand how widespread such relationship was its epigenetic biological correlates, systematically looked genes with similar transcription profiles analyzed their DNA methylation chromatin state accessibility many different cell populations. Twenty were expressed preferentially cerebellum (Myob/Cbl genes). Some shared hypo- or...
Previous studies have demonstrated the utility of microarray expression analysis to identify potential microRNA targets. Nevertheless, technical limitations intrinsic this platform constrain its ability fully exploit assessing transcript level changes explore targetomes. High-throughput multiplexed Illumina-based next-generation sequencing (NGS) provides a digital readout absolute levels and imparts higher accuracy dynamic range than platforms. We used Illumina NGS analyze transcriptome...