A5057768137- RNA Research and Splicing
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- IL-33, ST2, and ILC Pathways
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Estrogen and related hormone effects
- SARS-CoV-2 detection and testing
- Immune Cell Function and Interaction
- Carbohydrate Chemistry and Synthesis
- Biosensors and Analytical Detection
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Enzyme Structure and Function
- Pluripotent Stem Cells Research
- Alzheimer's disease research and treatments
- Autophagy in Disease and Therapy
University at Albany, State University of New York
2014-2023
Albany State University
2009-2023
Cleveland Clinic
2022
Cancer Research Center
2013-2021
State University of New York
2015-2021
SUNY Polytechnic Institute
2019
Rensselaer Polytechnic Institute
2013-2015
Purdue University West Lafayette
1998-2013
University of Pennsylvania
2006
Urbana University
2006
Tristetraprolin (TTP) is an RNA-binding protein required for the rapid degradation of mRNAs containing AU-rich elements. Targets regulated by TTP include encoding tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor, interleukin-2 (IL-2), and immediate early response 3. To identify novel target in macrophages, we used a genome-wide approach that combines RNA immunoprecipitation microarray analysis. A list was compiled 137 are associated with estimated accuracy on...
Summary In a variety of chronic pain conditions, calcitonin gene-related peptide (CGRP) is expressed at high levels in the skin, blood, and cerebrospinal fluid. Our results reveal that much increase may be due to CGRPβ isoform made by epidermal keratinocytes, which express CGRP immunoreactivity human animal conditions. Calcitonin vasodilatory has been detected fluid (CSF) under inflammatory presumably derived from peptidergic C Aδ innervation. Herein, immunolabeling (IL) was keratinocytes...
There is evidence from epidemiological and in vitro studies that the biological effects of testosterone (T) on cell cycle survival are modulated by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) prostate cancer. To investigate cross talk between androgen- vitamin D-mediated intracellular signaling pathways, individual combined T 1,25(OH)2D3 global gene expression LNCaP cancer cells were assessed.Stringent statistical analysis identifies a cohort genes lack one or both androgen response elements...
Pomegranate extract (PE) inhibits the proliferation of breast cancer cells and stimulates apoptosis in MCF-7 cells. While PE is a potent antioxidant, present studies were conducted to examine mechanisms action beyond antioxidation by studying cellular molecular underlying tumorigenesis. inhibited cell growth inducing cycle arrest G2/M followed induction apoptosis. In contrast, antioxidants N-acetylcysteine Trolox did not affect at doses containing equivalent antioxidant capacity as PE,...
ABSTRACT The vitamin D receptor (VDR) is expressed in human adipocytes and transiently induced during early adipogenesis mesenchymal progenitor cell models. VDR null mice exhibit enhanced energy expenditure reduced adiposity even when fed high fat diets. Adipocyte‐specific transgenic‐expression of enhances adipose tissue mass, indicating that activation lipid storage vivo. In these studies, we conducted genomic profiling differentiation assays primary cultures adipose‐derived cells to define...
Despite mounting evidence suggesting the involvement of immune system in regulating brain function, specific role and inflammatory cells neurodegenerative diseases remain poorly understood. In this study, we report that depletion NK cells, a type innate lymphocytes, alleviates neuroinflammation, stimulates neurogenesis, improves cognitive function triple-transgenic Alzheimer disease (AD) mouse model. brains AD model (3xTg-AD) mice exhibited an enhanced proinflammatory profile. Depletion by...
Increasing evidence has challenged the traditional view about immune privilege of brain, but precise roles cells in regulating brain physiology and function remain poorly understood. Here, we report that tissue-resident group 2 innate lymphoid (ILC2) accumulate choroid plexus aged brains. ILC2 are long-lived, relatively resistant to cellular senescence exhaustion, capable switching between cell cycle dormancy proliferation. They functionally quiescent at homeostasis can be activated by IL-33...
Muscle wasting (cachexia) is a consequence of chronic diseases, such as cancer, and associated with degradation muscle proteins MyoD. The cytokines tumor necrosis factor alpha gamma interferon induce degeneration by activating the transcription NF-kappaB its target genes. Here, we show that downstream nitric oxide (NO) synthase gene (iNos) suggest NO production stimulates MyoD mRNA loss. In fact, although cytokine treatment iNos(-/-) mice activated NF-kappaB, it did not trigger degeneration,...
Abstract Background Facioscapulohumeral muscular dystrophy (FSHD) is a dominant disease linked to contraction of an array tandem 3.3-kb repeats (D4Z4) at 4q35. Within each repeat unit gene, DUX4 , that can encode protein containing two homeodomains. A transcript derived from the last in contracted associated with pathogenesis but it unclear how. Methods Using exon-based microarrays, expression profiles myogenic precursor cells were determined. Both undifferentiated myoblasts and...
<b><i>Background/Aims:</i></b> Methionine restriction (MR) is a dietary intervention that increases lifespan, reduces adiposity and improves insulin sensitivity. These effects are reversed by supplementation of the MR diet with cysteine (MRC). Genomic metabolomic studies were conducted to identify potential mechanisms which induces favorable metabolic effects, supplementation. <b><i>Methods:</i></b> Gene expression was examined microarray...
Abstract Objective —To evaluate global genome expression patterns of mitral valve tissues from dogs with degenerative disease (DMVD). Sample Population —Anterior leaflets 4 severe DMVD and healthy control dogs. Procedures —Transcriptional activities 23,851 canine DNA sequences were determined by use an oligonucleotide microarray. Genome tissue evaluated measuring the relative amount complementary RNA hybridization to microarray probes comparing it gene Results —229 transcripts differentially...
Significance Proinflammatory diseases, such as cancer, AIDS, and chronic obstructive pulmonary are often associated with a progressive loss of skeletal muscle tissue, syndrome also known cachexia. Cytokines trigger by activating downstream effector pathways, including the ones driven STAT3 protein. Although high levels protein required for onset wasting, mechanisms modulating expression in cachectic muscles remain elusive. Here we identify RNA-binding HuR its ability to interfere miR-330...
Whiteface Mountain (WFM) in northern NY State is the site of a historic mountaintop atmospheric observatory with an ongoing cloud water chemistry monitoring program that has been operating every summer (June through September) since 1994. Though long-term chemical analysis conducted, no on microbiome completed at WFM. Over years, new regime reported cloudwater missing analytes. Knowing how microbes can interact chemicals, we hypothesize are partially responsible for this shift and crucial...
Acivicin [(αS,5S)-α-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid] was investigated as an inhibitor of the triad glutamine amidotransferases, IGP synthase and GMP synthetase. Nucleophilic substitution chlorine atom in acivicin results formation imine−thioether adduct at active site cysteine. Cys 77 identified modification heterodimeric IGPS from Escherichia coli (HisHF) by tryptic digest FABMS. Distinctions glutaminase domains E. coli, bifunctional protein Saccharomyces cerevisiae...
Throughout the vertebrate subphylum, regenerative potential of central nervous system axons is greatest in embryonic stages and declines as development progresses. For example, Xenopus laevis can functionally recover from complete transection spinal cord a tadpole but unable to do so after metamorphosing into frog. Neurons reticular formation raphe nucleus are among those that regenerate most reliably lose this ability metamorphosis. To identify molecular factors associated with success...
Protein synthesis and degradation are posttranscriptional pathways used by cells to regulate protein levels. We have developed a systems biology approach identify targets of regulation we employed this system in Saccharomyces cerevisiae study the DNA damage response. present evidence that 50% 75% transcripts induced alkylation regulated posttranscriptionally. Significantly, demonstrate two transcriptionally-induced response genes, RNR1 RNR4, fail show soluble level increases after damage. To...
Germline stem cell (GSC) self-renewal and differentiation are required for the sustained production of gametes. GSC in Drosophila oogenesis requires expression histone methyltransferase dSETDB1 by somatic niche, however its function this process is unknown. Here, we show that a Wnt ligand, Wingless type mouse mammary virus integration site number 4 (dWnt4) niche. dWnt4 signaling acts on niche cells to facilitate their encapsulation daughter, which serves as cue. known repress transposable...
Eukaryotic gene expression is a complex process involving multiple cis and trans activating molecules to either facilitate or inhibit transcription. In recent years, many studies have focused on the role of acetylation histone proteins in modulating transcription, whereas deacetylation these same associated with inactivation repression expression. This study explores HepG2 F9 cell lines treated Trichostatin A (TSA), potent deacetylase inhibitor.These experiments show that TSA treatment...
Bruton's tyrosine kinase (BTK) is a non-receptor that has mainly been studied in haematopoietic cells. We have investigated whether BTK potential therapeutic target prostate cancer. find expressed cells, with the alternate BTK-C isoform predominantly cancer cells and tumors. This transcribed from an alternative promoter results protein amino-terminal extension. Prostate cell lines tumors express more transcript than malignant NAMALWA B-cell line or human lymphomas. expression also observed...