A5064805798- RNA Research and Splicing
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Animal Genetics and Reproduction
- Invertebrate Immune Response Mechanisms
- Reproductive Biology and Fertility
- Bacteriophages and microbial interactions
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics, Aging, and Longevity in Model Organisms
- Autophagy in Disease and Therapy
- Nuclear Structure and Function
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- Neurobiology and Insect Physiology Research
- Immune cells in cancer
Icahn School of Medicine at Mount Sinai
2022-2024
New York Stem Cell Foundation
2022-2024
University at Albany, State University of New York
2016-2023
Stem Cell Institute
2023
Albany State University
2021
New York University
2007-2011
Howard Hughes Medical Institute
2011
Johns Hopkins University
2003-2009
University of Maryland, Baltimore
2004
National Institute of Standards and Technology
2004
Compact but non-native intermediates have been implicated in the hierarchical folding of several large RNAs, there is little information on their structure. In this article, ribonuclease and hydroxyl radical cleavage protection assays showed that base pairing core helices stabilize a compact state small group I ribozyme from Azoarcus pre-tRNA ile . Base requires 10-fold less Mg 2+ than stable tertiary interactions, indicating assembly catalytic represents distinct phase precedes formation...
Abstract Oocytes are arrested for long periods of time in the prophase first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, mechanisms regulating transcriptional activity I-arrested oocyte still not entirely understood. We hypothesized that expression during I arrest is primarily epigenetically regulated. Here we comprehensively define Drosophila female germ line epigenome throughout oogenesis and show has a unique, dynamic...
Oocyte specification is a critical developmental transition that requires the coordinated repression of germ cell-specific genes and activation maternal program to support embryogenesis. In Drosophila, timely cell early oogenesis essential for this transition, yet mechanisms coordinate process remain unclear. Here, we uncover an unexpected translation-chromatin axis, where transient Target Rapamycin Complex 1 (TORC1)-driven translation triggers chromatin remodeling, ensuring irreversible...
Germline stem cell (GSC) self-renewal and differentiation are required for the sustained production of gametes. GSC in Drosophila oogenesis requires expression histone methyltransferase dSETDB1 by somatic niche, however its function this process is unknown. Here, we show that a Wnt ligand, Wingless type mouse mammary virus integration site number 4 (dWnt4) niche. dWnt4 signaling acts on niche cells to facilitate their encapsulation daughter, which serves as cue. known repress transposable...
Highlights•Ski complex mediated RNA degradation is required in the germline for fertility.•A subset of early oogenic RNAs are degraded concurrent with oocyte specification.•Early utilizing components No Go Decay pathway.•Degradation maintenance fate.SummaryIn sexually reproducing animals, contributes a large supply that essential to launch development upon fertilization. The mechanisms regulate composition maternal contribution during oogenesis unclear. Here, we show expressed stages...
Maternal mRNAs synthesized during oogenesis initiate the development of future generations. Some maternal are either somatic or germline determinants and must be translationally repressed until embryogenesis. However, translational repressors themselves temporally regulated. We used polar granule component (pgc), a Drosophila mRNA, to ask how transcripts while regulatory landscape is shifting. pgc, determinant, regulated throughout oogenesis. find that different conserved RNA-binding...
Emerging evidence suggests that ribosome heterogeneity may have important functional consequences in the translation of specific mRNAs within different cell types and under various conditions. Ribosome comes many forms, including post-translational modification proteins (RPs), absence RPs inclusion RP paralogs. The Drosophila genome encodes two RpS5 paralogs: RpS5a RpS5b. While is ubiquitously expressed, RpS5b exhibits enriched expression reproductive system. Deletion results female...
ABSTRACT Gamete formation from germline stem cells (GSCs) is essential for sexual reproduction. However, the regulation of GSC differentiation incompletely understood. Set2, which deposits H3K36me3 modifications, required during Drosophila oogenesis. We discovered that reader Male-specific lethal 3 (Msl3) and histone acetyltransferase complex Ada2a-containing (ATAC) cooperate with Set2 to regulate in female Drosophila. Msl3, acting independently rest male-specific complex, promotes...
Genome organization can regulate gene expression and promote cell fate transitions. The differentiation of germline stem cells (GSCs) to oocytes in
Counterions are critical to the self-assembly of RNA tertiary structure because they neutralize large electrostatic forces which oppose folding process. Changes in size and shape Azoarcus group I ribozyme as a function Mg(2+) Na(+) concentration were followed by small angle neutron scattering. In low salt buffer, was expanded, with an average radius gyration (R(g)) 53 +/- 1 A. A highly cooperative transition compact form (R(g) = 31.5 0.5 A) observed between 1.6 1.7 mM MgCl(2). The collapse...
Germline stem cells (GSCs) self-renew and differentiate to sustain a continuous production of gametes. In the female Drosophila germ line, two differentiation factors, bag marbles ( bam) benign gonial cell neoplasm bgcn), work in concert daughter promote generation eggs. GSCs, bam transcription is repressed by signaling from niche activated daughters. contrast, bgcn transcribed both GSCs daughters, but little known about how transcriptionally modulated. Here we find that conserved protein...
Article23 March 2022Open Access Source DataTransparent process Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila Shamsi Emtenani orcid.org/0000-0001-6981-6938 Institute of Science Technology Austria, Klosterneuburg, Austria Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft Search for more papers this author Elliot T Martin Department Biological Sciences, RNA Institute,...
Stem cells in many systems, including
The association of genomic loci to the nuclear periphery is proposed facilitate cell type-specific gene repression and influence fate decisions. However, interplay between position expression remains incompletely understood, in part because proteins that at remain unidentified. Here, we used an Oligopaint-based HiDRO screen targeting ∼1000 genes discover novel regulators architecture