A5064385054- RNA Research and Splicing
- RNA modifications and cancer
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Developmental Biology and Gene Regulation
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Photosynthetic Processes and Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Protein Kinase Regulation and GTPase Signaling
- Metabolism and Genetic Disorders
- Neurobiology and Insect Physiology Research
- Environmental DNA in Biodiversity Studies
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Plant Molecular Biology Research
University of Lisbon
2018-2024
Instituto de Medicina Molecular João Lobo Antunes
2018-2024
Algarve Biomedical Center
2018-2021
University of Algarve
2014-2021
Hospital de Faro EPE
2016
Instituto Gulbenkian de Ciência
2012-2015
Institute for Biotechnology and Bioengineering
2014
Abstract Oocytes are arrested for long periods of time in the prophase first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, mechanisms regulating transcriptional activity I-arrested oocyte still not entirely understood. We hypothesized that expression during I arrest is primarily epigenetically regulated. Here we comprehensively define Drosophila female germ line epigenome throughout oogenesis and show has a unique, dynamic...
Epithelial cells mostly orient the spindle along plane of epithelium (planar orientation) for mitosis to produce two identical daughter cells. The correct orientation relies on interaction between cortical polarity components and astral microtubules. Recent studies in mammalian tissue culture suggest that apically localised atypical protein kinase C (aPKC) is important planar mitotic dividing epithelial Yet, chicken neuroepithelial cells, aPKC not required vivo orientation, it has been...
Drosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the embryo, being avoidance a possible explanation why most genes are intronless. We isolated two mutant alleles for subunit NTC/Prp19 complexes, which specifically impaired maternally encoded transcripts. hypothesized requirements efficiency vary during...
Protein N-terminal acetylation is an ancient and ubiquitous co-translational modification catalyzed by a highly conserved family of acetyltransferases (NATs). Prokaryotes have at least 3 NATs, whereas humans six distinct but suggesting increase in regulatory complexity this during eukaryotic evolution. Despite this, against our initial expectations, we determined that NAT diversification did not occur the eukaryotes, as all major human NATs were most likely present Last Eukaryotic Common...
Male germ cells share a common origin across animal species, therefore they likely retain conserved genetic program that defines their cellular identity. However, the unique evolutionary dynamics of male coupled with widespread leaky transcription pose significant obstacles to identification core spermatogenic program. Through network analysis spermatocyte transcriptome vertebrate and invertebrate we describe metazoan at molecular level. We estimate average functional requirement cell...
Widespread cotranscriptional splicing has been demonstrated from yeast to human. However, most studies date addressing the kinetics of relative transcription used either
Mammalian Native Elongating Transcript sequencing (mNET-seq) is a recently developed technique that generates genome-wide profiles of nascent transcripts associated with RNA polymerase II (Pol II) elongation complexes. The ternary transcription complexes formed by Pol II, DNA template and are first isolated, without crosslinking, immunoprecipitation antibodies specifically recognize the different phosphorylation states large subunit C-terminal domain (CTD). coordinate 3′ end in then...
Summary Male germ cells share a common origin across animal species, therefore they likely retain conserved genetic program that defines their cellular identity. However, the unique evolutionary dynamics of male coupled with widespread leaky transcription pose significant obstacles to identification core spermatogenic program. Through network analysis spermatocyte transcriptome vertebrate and invertebrate we describe metazoan at molecular level. We estimate average functional requirement...
Male germ cells share a common origin across animal species, therefore they likely retain conserved genetic program that defines their cellular identity. However, the unique evolutionary dynamics of male coupled with widespread leaky transcription pose significant obstacles to identification core spermatogenic program. Through network analysis spermatocyte transcriptome vertebrate and invertebrate we describe metazoan at molecular level. We estimate average functional requirement cell...
Male germ cells share a common origin across animal species, therefore they likely retain conserved genetic program that defines their cellular identity. However, the unique evolutionary dynamics of male coupled with widespread leaky transcription pose significant obstacles to identification core spermatogenic program. Through network analysis spermatocyte transcriptome vertebrate and invertebrate we describe metazoan at molecular level. We estimate average functional requirement cell...
Male germ cells share a common origin across animal species, therefore they likely retain conserved genetic program that defines their cellular identity. However, the unique evolutionary dynamics of male coupled with widespread leaky transcription pose significant obstacles to identification core spermatogenic program. Through network analysis spermatocyte transcriptome vertebrate and invertebrate we describe metazoan at molecular level. We estimate average functional requirement cell...
The NineTeen Complex (NTC), also known as pre-mRNA-processing factor 19 (Prp19) complex, regulates distinct spliceosome conformational changes necessary for splicing. During Drosophila midblastula transition, splicing is particularly sensitive to mutations in NTC-subunit Fandango, which suggests differential requirements of NTC during development. We show that Salsa, the ortholog human RNA helicase Aquarius, rate-limiting a subset small first introns oogenesis, including intron gurken...
Epithelial cells mostly orient the spindle along plane of epithelium (planar orientation) for mitosis to produce two identical daughter cells.The correct orientation relies on interaction between cortical polarity components and astral microtubules.Recent studies in mammalian tissue culture suggest that apically localised atypical protein kinase C (aPKC) is important planar mitotic dividing epithelial cells.Yet, chicken neuroepithelial cells, aPKC not required vivo orientation, it has been...
ABSTRACT The transition from fertilized oocyte to totipotent embryo relies on maternally-provided factors that are synthetized and accumulated in developing oocytes. Yet, it is still unclear how oocytes regulate the expression of these fate-promoting genes within general transcriptional program oogenesis. Here we report Drosophila Trithorax group protein MLL3/4 (dMLL3/4, also known as Trr) essential for fate at fertilization. In absence dMLL3/4, develop normally but fail initiate mitotic...
ABSTRACT Widespread co-transcriptional splicing has been demonstrated from yeast to human. However, most studies date addressing the kinetics of relative transcription used either Saccharomyces cerevisiae or metazoan cultured cell lines. Here, we adapted native elongating transcript sequencing technology (NET-seq) measure dynamics during early developmental stages Drosophila melanogaster embryos. Our results reveal position RNA polymerase II (Pol II) when both canonical and recursive occur....