A5010732653- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Hormonal and reproductive studies
- Sexual Differentiation and Disorders
- Testicular diseases and treatments
- Reproductive Health and Technologies
- Genital Health and Disease
- Reproductive System and Pregnancy
- Medical and Health Sciences Research
- Immunotherapy and Immune Responses
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Urologic and reproductive health conditions
- Epigenetics and DNA Methylation
- Urological Disorders and Treatments
- Ovarian function and disorders
- Male Reproductive Health Studies
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Pharmacogenetics and Drug Metabolism
- Pharmacology and Obesity Treatment
- Dermatology and Skin Diseases
- Health and Medical Studies
- Male Breast Health Studies
- Immune Cell Function and Interaction
- COVID-19 Impact on Reproduction
Justus-Liebig-Universität Gießen
2014-2024
Klinik und Poliklinik für Urologie, Kinderurologie und Andrologie
2011-2024
Klinik und Poliklinik für Urologie
2024
Universitätsklinikum Gießen und Marburg
2011-2023
Giessen School of Theology
2011-2014
Friedrich-Alexander-Universität Erlangen-Nürnberg
2006
Joint Research Centre
2006
Friedrich Schiller University Jena
2006
Heinrich Heine University Düsseldorf
1997
Darmstadt University of Applied Sciences
1995
Which immune cells and cytokine profiles are characteristic for testicular germ cell neoplasia what consequences does this have the understanding of related immunopathology? The unique environment comprises B dendritic as well high transcript levels IL-6 other supporting or T helper type 1 (Th1)-driven cytokines thus differs profoundly from normal testis inflammatory lesions associated with hypospermatogenesis. known to be major component infiltrates either hypospermatogenesis cancer. It has...
Could the protamine-1 to protamine-2 mRNA ratio serve as a biomarker estimate fertilizing capacity of sperm from men taking part in an IVF/ICSI programme? The protamine clearly discriminates between fertile and subfertile with normal exhibit higher IVF/ICSI. Aberrant ratios are associated male factor infertility is comparable protein (due transcriptional stop elongating spermatids). study population was drawn men, whose female partners participated IVF or ICSI programmes September 2010...
Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility scientific publications. This even important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition WHO Laboratory Manual Examination Processing Human Semen, International Standard ISO 23162:2021. With these tools, we propose authors should be instructed to follow laboratory methods order publish...
Sperm chromatin reveals two characteristic features in that protamines are the predominant nuclear proteins and remaining histones highly acetylated. Histone H4 acetylated at lysine 12 (H4K12ac) is localized post-acrosomal region, while protamine-1 present within whole nucleus. Chromatin immunoprecipitation combination with promoter array analysis allowed genome-wide identification of H4K12ac binding sites. Previously, we reported enrichment CTCF sites promoters genes involved developmental...
Histone to protamine exchange and the hyperacetylation of remaining histones are hallmarks spermiogenesis. Acetylation histone H4 at lysine 12 (H4K12ac) was observed prior full decondensation sperm chromatin after fertilization suggesting an important role for regulation gene expression in early embryogenesis. Similarly, DNA methylation may contribute silencing several developmentally genes. Following identification H4K12ac-binding promoters fertile subfertile patients, we aimed investigate...
Male germ cells share a common origin across animal species, therefore they likely retain conserved genetic program that defines their cellular identity. However, the unique evolutionary dynamics of male coupled with widespread leaky transcription pose significant obstacles to identification core spermatogenic program. Through network analysis spermatocyte transcriptome vertebrate and invertebrate we describe metazoan at molecular level. We estimate average functional requirement cell...
The effects of age on semen quality were analysed in patients referred to an andrology outpatient clinic a retrospective study covering period 3 years. Semen analyses older men (n = 66; > or 50 years; median: 53) compared with those young 134; 21-25 median 24). duration sexual abstinence was longer among (median: 5.5 vs. 5.0 days; + 10%, P < 0.05). Even after adjustment for abstinence, progressive motility (- 27%, 0.01), percentage morphologically normal spermatozoa 44%, 0.01) and volume...
To investigate age-related inflammatory events in the male genital tract.In a total of 4265 randomly collected patients attending andrological outpatient clinic Center for Dermatology and Andrology, University Giessen, Germany, ejaculate volume, pH-value, sperm concentration, progressive motility, concentration polymorphonuclear (PMN) elastase, number peroxidase-positive cells fructose were measured correlated with patient's age.While motility all negatively age, PMN elastase pH-value showed...
During spermatogenesis, approximately 85% of histones are replaced by protamines. The remaining have been proposed to carry essential marks for the establishment epigenetic information in offspring. aim present study was analyse expression pattern histone H3 acetylated at lysine 9 (H3K9ac) during normal and impaired spermatogenesis binding H3K9ac selected genes within ejaculates. Testicular biopsies, as well semen samples, were used immunohistochemistry. Chromatin immunoprecipitation...
Abstract Background Immune cell infiltration is heterogeneous but common in testicular germ tumors (TGCT) and pre-invasive neoplasia situ (GCNIS). Tumor-infiltrating T cells including regulatory (Treg) follicular helper (Tfh) are found other cancer entities, their contributions to TGCT unknown. Methods Human testis specimens from independent patient cohorts were analyzed using immunohistochemistry, flow cytometry single-cell RNA sequencing (scRNA-seq) with special emphasis on delineating...
Does the chemokine/chemokine receptor axis, involved in immune cell trafficking, contribute to pathology of testicular inflammation and how does activin A modulate this network?
Abstract Introduction Supporting and counselling couples with fertility issues prior to starting ART is a multidisciplinary diagnostic therapeutic challenge. The first German/Austrian/Swiss interdisciplinary S2k guideline on “Diagnosis Therapy Before Assisted Reproductive Treatments (ART)” was published in February 2019. This developed the context of guidelines program German Society Gynecology Obstetrics (DGGG) cooperation Swiss (SGGG) Austrian (OEGGG). Aims One third causes involuntary...
Silent chronic inflammation of genital tract (CIGT) is considered as a major contributing factor to male fertility disorders. Within CIGT, inflammatory cytokines such TNF-alpha might induce spermatozoal apoptosis, which in turn has been shown have negative influence on the sperm-oocyte penetration capacity. Thus, aim this study was investigate apoptosis patients with disorders and signs CIGT. Apoptosis spermatozoa determined by expression annexin V using flow cytometry. Apoptotic were...