A5043257601- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Effects and risks of endocrine disrupting chemicals
- Ovarian function and disorders
- Carcinogens and Genotoxicity Assessment
- Air Quality and Health Impacts
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Pesticide Exposure and Toxicity
- Reproductive biology and impacts on aquatic species
- Health, Environment, Cognitive Aging
- Neuroendocrine regulation and behavior
- Toxic Organic Pollutants Impact
- Hormonal and reproductive studies
- Animal Genetics and Reproduction
- Prenatal Screening and Diagnostics
- Sexual Differentiation and Disorders
- Chromosomal and Genetic Variations
- Plant Taxonomy and Phylogenetics
- Glutathione Transferases and Polymorphisms
- Environmental Toxicology and Ecotoxicology
- Climate Change and Health Impacts
- Estrogen and related hormone effects
- Selenium in Biological Systems
- Pluripotent Stem Cells Research
Environmental Protection Agency
2003-2022
National Institute of Environmental Health Sciences
2009-2020
National Institutes of Health
2004-2020
Triangle
2017-2018
Research Triangle Park Foundation
2002-2015
Health Affairs
2015
Cambridge University Press
2011
New York University Press
2011
Chitose Institute of Science and Technology
2011
National Center for Environmental Assessment (EPA)
2009
Although glycolysis is highly conserved, it remarkable that several unique isozymes in this central metabolic pathway are found mammalian sperm. Glyceraldehyde 3-phosphate dehydrogenase-S (GAPDS) the product of a mouse gene expressed only during spermatogenesis and, like its human ortholog (GAPD2), sole GAPDH isozyme It tightly bound to fibrous sheath, cytoskeletal structure extends most length sperm flagellum. We disrupted Gapds expression by targeting selectively block and assess relative...
This article reports the results of most recent in a series EHSRE workshops designed to synthesize current state field Andrology and provide recommendations for future work (for details see Appendix). Its focus is on methods detecting sperm DNA damage potential application new knowledge about chromatin organization, vulnerability repair improve diagnosis treatment clinical infertility associated with that damage. Equally important use reliability these tests identify extent which...
BACKGROUND: This study examined potential associations between exposure to episodes of air pollution and alterations in semen quality. The pollution, resulting from combustion coal for industry home heating the Teplice district Czech Republic, was much higher during winter than at other times year with peaks exceeding US quality standards. METHODS: Young men were sampled up seven over 2 years allowing evaluation after periods both low high pollution. Routine analysis (sperm concentration,...
This study of male reproductive health in the Czech Republic resulted from community concern about potential adverse effects air pollution. We compared young men (18 years age) living Teplice, a highly industrialized district with seasonally elevated levels pollution, to those Prachatice, rural relatively clean air. Surveys were scheduled for either late winter, after season higher or at end summer, when pollution was low. Participation included physical examination, donation semen sample,...
To determine whether moderate cigarette smoking and alcohol consumption in teenage men is associated with increases disomic sperm detectable changes semen quality.Cohort study.Military recruiting station, Teplice, Czech Republic.Ten current smokers (20 cigarettes per day for at least 2 years, exposure confirmed by urine cotinine) who also consumed 15 nonsmokers. All patients were exactly 18 years old, healthy, of unproven fertility.Sperm aneuploidy multicolor fluorescence situ hybridization...
To assess the structural stability of mammalian sperm nuclei and make interspecies comparisons, we microinjected from six different species into hamster oocytes monitored occurrence nuclear decondensation male pronucleus formation. The time course varied considerably by species: human mouse decondensed within 15 to 30 min injection, chinchilla did so 45 60 min, but bull rat remained intact over this same period time. Male pronuclei formed in injected with human, mouse, chinchilla, nuclei,...
BackgroundResearch has suggested an association with ambient air pollution and sperm quality.ObjectivesWe investigated the effect of exposure to ozone (O3) particulate matter < 2.5 μm in aerodynamic diameter (PM2.5) on quality.MethodsWe reexamined a previous cohort study water disinfection by-products evaluate quality 228 presumed fertile men different profiles. Outcomes included concentration, total per ejaculate (count), morphology, as well DNA integrity chromatin maturity. Exposures O3...
Sources of variability in the zona-free hamster egg penetration assay were evaluated. Internal consistency was examined replicate experiments using sperm from 23 donors. The average difference percentage fertilization between replicates 3.9%. Optimal preincubation conditions and insemination time shown to be 0.5 1.0 X 10(7) sperm/ml 2 3 hours. Abstinence found a variable, critical abstinence more than 12 hours required. Prolonged exposure seminal plasma (i.e., 30 minutes) produced reduction...
The relationship between the timing of both sperm nuclear decondensation and male pronucleus formation in oocyte relative level disulfide bonds within nucleus was evaluated. Since reduction (S-S) is a prerequisite for vitro vivo, we hypothesized that nuclei with relatively few S-S would require less time to decondense than higher numbers bonds, occur more rapidly as well. Four types hamster nuclei, which extent bonding differed, were microinjected into oocytes, course charted. Cauda...
Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility scientific publications. This even important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition WHO Laboratory Manual Examination Processing Human Semen, International Standard ISO 23162:2021. With these tools, we propose authors should be instructed to follow laboratory methods order publish...
We hypothesized that depletion of glutathione (GSH) with diamide, a relatively specific GSH oxidant, may alter the meiotic spindle apparatus in mature hamster oocytes. Immunofluorescent analysis oocytes exposed to diamide for 1.5 or 3 h revealed time- and concentration-dependent disruption morphology accompanied by chromosome clumping. In first cultured then diamide-free medium h, microtubules appeared repolymerize, but normal structure was not regained. HPLC confirmed oxidized oocyte under...
Abstract Glutathione (GSH) is thought to play critical roles in oocyte function including spindle maintenance and provision of reducing power needed initiate sperm chromatin decondensation. Previous observations that GSH concentrations are higher mature than immature oocytes decline after fertilization, suggest synthesis may be associated with cell cycle events. To explore this possibility, we measured the Golden Hamster zygotes at specific stages maturation intervals during first complete...
Nuclei isolated from spermatozoa of various species (golden hamster, mouse, human, rooster, and the fish tilapia) were heated at 60°-125°C for 20–120 min then microinjected into hamster oocytes to determine whether they could decondense develop pronuclei. Mature, mammalian sperm nuclei, which are stabilized by protamine disulfide bonds, moderately heat resistant. For example, remained capable pronucleus formation even after pretreatment 30 90°C. Indeed, a temperature 125°C (steam) was...
This study determined the quantitative and qualitative histopathologic effects of a single oral dose 1,3-dinitrobenzene (48 mg/kg) on rat testis from 1 to 175 days postexposure. The was damaged severely by hour 24, as evidenced increased numbers regressive seminiferous tubules that exhibited degenerating pachytene spermatocytes, chromatin margination in spermatids, giant cells, deformed spermatid heads, retained reduced meiotic figures. major during first 48 hours posttreatment were...