A5047755964- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Estrogen and related hormone effects
- Prostate Cancer Treatment and Research
- Renal and related cancers
- Ubiquitin and proteasome pathways
- Mass Spectrometry Techniques and Applications
- Ovarian function and disorders
- Hormonal and reproductive studies
- Urological Disorders and Treatments
- Animal Genetics and Reproduction
- Sexual Differentiation and Disorders
- Reproductive biology and impacts on aquatic species
- Toxoplasma gondii Research Studies
- Effects and risks of endocrine disrupting chemicals
- Hormonal Regulation and Hypertension
- Urologic and reproductive health conditions
- Asthma and respiratory diseases
- Metabolism, Diabetes, and Cancer
- Caveolin-1 and cellular processes
- Reproductive System and Pregnancy
- Respiratory and Cough-Related Research
- Testicular diseases and treatments
University of Florence
2015-2024
University of Siena
2014
Meyer Children's Hospital
2013
Società Italiana di Cardiologia
2000-2003
Harvard University
1994
University of Modena and Reggio Emilia
1989-1990
Sperm DNA fragmentation (sDF) represents a threat to male fertility, human reproduction and the health of offspring. The causes sDF are still unclear, even if apoptosis, oxidative assault defects in chromatin maturation hypothesized. Using multicolor flow cytometry sperm sorting, we challenged three hypothesized mechanisms by simultaneously evaluating signs damage (8-hydroxy, 2′-deoxyguanosine (8-OHdG) malondialdehyde (MDA)), apoptosis (caspase activity cleaved poly(ADP-ribose) polymerase...
Does CatSper have a role in the achievement of human sperm motility and Progesterone (P)-induced acrosome reaction (AR)? CatSper1 expression is associated with progressive P-induced AR; it may pathogenesis asthenozoospermia. Knockout mice for any Catsper family genes fail to acquire hyperactivated are infertile. channels mediate Ca2+ influx sperm. The hyperactivated/activated asthenospermia less clear. A few men mutations been described but phenotype regarding has not well established....
Does the sperm DNA fragmentation index (DFI) improve depending on FSH receptor (FSHR) genotype as assessed by nonsynonymous polymorphisms rs6166 (p.N680S) after 3 months of recombinant treatment in men with idiopathic infertility? significantly improves DFI only infertile p.N680S homozygous N FSHR. FSH, fundamental for spermatogenesis, is empirically used to treat male infertility and several studies suggest that could be a candidate predictor response treatment, terms probability conceive....
Abstract Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation (DGC), a common selection procedure in Assisted Reproduction Techniques (ARTs), experience 50% lower probability of pregnancy. Hence, identification these subjects is clinical importance. Here, we investigated whether such are identified with higher accuracy detecting viable (viable sDF) instead total spermatozoa (total and swim up, an alternative to DGC, does not increase sDF. With 10/20 sDF, 2...
The functional significance of deoxyribonucleic acid (DNA) fragmentation in ejaculated human sperm is unclear. In this study the extent DNA strand breakage swim-up selected spermatozoa was evaluated by terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling (TUNEL)-coupled flow cytometry and correlated with several morphological parameters. (mean = 11.07%+/-8.00%, range 0.79%-42.64%, n 140) positively related to abnormal morphology associated defects tail. A...
The presence of a novel functional estrogen receptor on the human sperm surface has been demonstrated by using different experimental approaches. Ligand blot analysis lysates, peroxidase-conjugated estradiol as probe, identified specific estradiol-binding protein approximately 29-kDa apparent molecular mass. same band was also revealed αH222 antibody, which is directed against steroid binding domain genomic receptor. biological effects were investigated analyzing calcium fluxes, tyrosine...
ABSTRACT: The origin and the meaning of DNA fragmentation in ejaculated human spermatozoa are not yet clear, although some hypotheses have been proposed. In present study, we used investigated sperm by terminal deoxynucleotidyl transferase‐mediated fluorescein‐dUTP nick end labeling (TUNEL)‐coupled flow cytometry to investigate that were selected swim‐up procedure incubated long‐term. addition, using detected annexin V binding assay propidium iodide staining, also studied membrane...
Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect DNA integrity and impact pregnancy rate (PR), we prospectively evaluated fragmentation (sDF) by TUNEL/PI, before after DGC. sDF was studied a cohort 90 infertile couples same day IVF/ICSI treatment. After DGC, increased 41 samples (Group A, median...
Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility scientific publications. This even important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition WHO Laboratory Manual Examination Processing Human Semen, International Standard ISO 23162:2021. With these tools, we propose authors should be instructed to follow laboratory methods order publish...
Prostate cancer cells may lose androgen-sensitivity after androgen ablation therapy, becoming highly invasive and metastatic. The biological mechanisms responsible for higher tumurogenicity of androgen-independent prostate carcinomas are not entirely known. We demonstrate that receptor regulation adhesion invasion through modulation alpha6beta4 integrin expression be one the molecular this phenomenon. found protein gene expressions alpha6 beta4 subunits were strongly reduced in...
ABSTRACT: The signaling pathways that characterize the process of capacitation human spermatozoa are still largely unknown. Modifications in lipid architecture sperm plasma membrane have been described from different species, including translocation phosphatidylserine (PS) inner to outer leaflet and increased phospholipid disorder membrane. In spermatozoa, however, results PS exposure controversial. present study, we used flow cytometry investigate both by Annexin V (Ann V) binding...
Sperm DNA fragmentation is a possible predictive parameter for male fertility status. The occurrence of M540 bodies in semen subfertile subjects affects flow cytometric investigations sperm. We set up new method to evaluate excluding bodies.DNA was evaluated by cytometry 75 both terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling (TUNEL, traditional method) and double staining with TUNEL propidium iodide (PI, method).The use the revealed that underestimates...
ABSTRACT: Techniques for assessing sperm DNA damage are numerous and various. There 2 main types of assay: direct indirect. The former directly detects the amount damage, whereas latter reveals effects an exogenous insult on chromatin. In addition, even considering same type technique, different strategies to reveal or quantify both, used. Finally, these techniques, except chromatin structure assay (SCSA), lack standardized protocols which all users can adhere minimize interlaboratory...
Oxidative stress (OS) is involved in many disoders including male infertility. Human spermatozoa are very sensitive targets of reactive oxygen species (ROS) and most sperm functions impaired the case OS. In addition unbalanced production ROS considered one important causes DNA fragmentation, a semen trait infertile men. The relationship between oxidative damage quality partially controversial, probably due to different methods and/or used reveal this study, by fluorescence microscopy flow...
STUDY QUESTIONIs CatSper1 expression in human spermatozoa related to semen parameter values and sperm functions?
Summary The aim of this study was to provide a comprehensive genetic/phenotypic characterization subjects suffering infertility owing sperm macrocephaly ( n = 3) or globozoospermia 9) and investigate whether the patients’ genetic status correlated with alteration various parameters. AURKC sequenced in case while DPY 19L2 has been analyzed by multiple approaches including novel qPCR –based copy number assay globozoospermia. Globozoospermic patients were also for SPACA 1 , candidate gene...