A5081731109- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Reproductive Health and Technologies
- Ovarian function and disorders
- Prostate Cancer Treatment and Research
- Neuroendocrine regulation and behavior
- Hormonal and reproductive studies
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
- Estrogen and related hormone effects
- Reproductive biology and impacts on aquatic species
- Neuropeptides and Animal Physiology
- Urologic and reproductive health conditions
- Nitric Oxide and Endothelin Effects
- Sexual Differentiation and Disorders
- Nicotinic Acetylcholine Receptors Study
- Hormonal Regulation and Hypertension
- Olfactory and Sensory Function Studies
- Circadian rhythm and melatonin
- COVID-19 Impact on Reproduction
- Prostate Cancer Diagnosis and Treatment
- Urinary Bladder and Prostate Research
- Ubiquitin and proteasome pathways
Marconi University
2024-2025
University of Florence
2015-2024
Azienda Ospedaliero-Universitaria Careggi
2021-2024
Universidade Estadual de Campinas (UNICAMP)
2016
University of Siena
2014
Meyer Children's Hospital
2013
Heidelberg University
2010
Tumour Institute of Tuscany
2010
Cambridge University Press
2010
New York University Press
2010
Sperm DNA fragmentation (sDF) represents a threat to male fertility, human reproduction and the health of offspring. The causes sDF are still unclear, even if apoptosis, oxidative assault defects in chromatin maturation hypothesized. Using multicolor flow cytometry sperm sorting, we challenged three hypothesized mechanisms by simultaneously evaluating signs damage (8-hydroxy, 2′-deoxyguanosine (8-OHdG) malondialdehyde (MDA)), apoptosis (caspase activity cleaved poly(ADP-ribose) polymerase...
How is the semen quality of sexually active men following recovery from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection?Twenty-five percent with recent SARS-Cov-2 infections and proven healing were oligo-crypto-azoospermic, despite absence virus RNA in semen.The presence SARS-CoV-2 human its role contagion after disease 2019 (COVID-19) still unclear. So far, studies evaluating occurrence infected or recovered are scarce included a limited number participants.A...
Liver perisinusoidal fat-storing cells (FSC) show morphological and ultrastructural characteristics similar to pericytes regulating local blood flow in other organs. In the present study we have analyzed whether FSC respond vasoconstrictors such as thrombin, angiotensin-II, endothelin-1 with an increase intracellular free calcium concentration ([Ca2+]i) coupled effective cell contraction. All agonists tested induced a rapid dose-dependent [Ca2+]i followed by sustained phase lasting several...
ABSTRACT: Progesterone induced a rapid, long‐lasting, dose‐dependent increase of intracellular free calcium concentration ([Ca2 +]i) in human sperm capacitated overnight. This effect was not counteracted by the cytosolic progesterone receptor antagonist RU486 (1 μmol/L) nor GABA‐A antagonists bicuculline (10 and picrotoxin (50 μmol/L). Also, rank order potency several progestative steroids on [Ca2 +]i differed from that previously reported for uterine or P‐GABA interaction central nervous...
On the basis of previous experimental evidence, it is known that auditory thalamus (AT), dorsal hippocampus (DH), basolateral amygdala (BLA), and perirhinal cortex (PC) are involved in mnemonic processing conditioned freezing. In particular, BLA PC appear to be both stimulus (CS) context Through AT, CS sent other sites, whereas DH conditioning. Nevertheless, existing evidence does not make possible assess DH, BLA, involvement during consolidation phase To address this question, fully...
Some cerebellar structures are known to be involved in the memorization of several conditioned responses. The role interpositus nucleus (IN) and vermis (VE) fear-conditioning consolidation was investigated by means a combined behavioral neurophysiological technique. IN VE were subjected fully reversible tetrodotoxin (TTX) inactivation during adult male Wistar rats that underwent acoustic stimulus (CS) context fear training. TTX injected different groups at increasing intervals after...
In the ample field of biological non-linear relationships there is also inverted U-shaped dose-effect. relation to cognitive functions, this phenomenon has been widely reported for many active compounds, in several learning paradigms, animal species and does not depend on either administration route (systemic or endocerebral) time (before after training). This review summarizes its most interesting aspects. The hypothesized mechanisms supporting it are discussed, with particular emphasis...
Does CatSper have a role in the achievement of human sperm motility and Progesterone (P)-induced acrosome reaction (AR)? CatSper1 expression is associated with progressive P-induced AR; it may pathogenesis asthenozoospermia. Knockout mice for any Catsper family genes fail to acquire hyperactivated are infertile. channels mediate Ca2+ influx sperm. The hyperactivated/activated asthenospermia less clear. A few men mutations been described but phenotype regarding has not well established....
Does the sperm DNA fragmentation index (DFI) improve depending on FSH receptor (FSHR) genotype as assessed by nonsynonymous polymorphisms rs6166 (p.N680S) after 3 months of recombinant treatment in men with idiopathic infertility? significantly improves DFI only infertile p.N680S homozygous N FSHR. FSH, fundamental for spermatogenesis, is empirically used to treat male infertility and several studies suggest that could be a candidate predictor response treatment, terms probability conceive....
Abstract Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation (DGC), a common selection procedure in Assisted Reproduction Techniques (ARTs), experience 50% lower probability of pregnancy. Hence, identification these subjects is clinical importance. Here, we investigated whether such are identified with higher accuracy detecting viable (viable sDF) instead total spermatozoa (total and swim up, an alternative to DGC, does not increase sDF. With 10/20 sDF, 2...
Abstract Background It is now 11 years since publication of the WHO 2010 guidelines for semen assessment values, and it critical to determine whether they are still valid and/or should be modified. Objectives To utilise data published combine these with used in provide an updated more comprehensive representation fertile man. This may utilised present distribution values use by 2021. Materials Methods Two specific analyses were performed namely, (1) Analysis 1: Examination following [termed...
The functional significance of deoxyribonucleic acid (DNA) fragmentation in ejaculated human sperm is unclear. In this study the extent DNA strand breakage swim-up selected spermatozoa was evaluated by terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling (TUNEL)-coupled flow cytometry and correlated with several morphological parameters. (mean = 11.07%+/-8.00%, range 0.79%-42.64%, n 140) positively related to abnormal morphology associated defects tail. A...
The presence of a novel functional estrogen receptor on the human sperm surface has been demonstrated by using different experimental approaches. Ligand blot analysis lysates, peroxidase-conjugated estradiol as probe, identified specific estradiol-binding protein approximately 29-kDa apparent molecular mass. same band was also revealed αH222 antibody, which is directed against steroid binding domain genomic receptor. biological effects were investigated analyzing calcium fluxes, tyrosine...
Capacitation of spermatozoa, a complex process occurring after sperm ejaculation, is required to obtain fertilization the oocyte in vivo and vitro. Although most biochemical/ biophysical events that occur during capacitation vitro have been characterized, molecular mechanisms underlying these are still obscure. Increases intracellular free Ca2+ concentrations ([Ca2+]i) protein tyrosine phosphorylation previously demonstrated human spermatozoa. In present study we investigated relationship...