A5067788619- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Selenium in Biological Systems
- Hormonal and reproductive studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cholesterol and Lipid Metabolism
- Lipid metabolism and biosynthesis
- Drug Transport and Resistance Mechanisms
- Ovarian function and disorders
- COVID-19 Impact on Reproduction
- Reproductive System and Pregnancy
- Silk-based biomaterials and applications
- Stress Responses and Cortisol
- Redox biology and oxidative stress
- Glutathione Transferases and Polymorphisms
- Social Policies and Family
- Reproductive biology and impacts on aquatic species
- Estrogen and related hormone effects
- Vitamin D Research Studies
- Neurobiology and Insect Physiology Research
- Tryptophan and brain disorders
- Immunotherapy and Immune Responses
- Plant Stress Responses and Tolerance
- Effects and risks of endocrine disrupting chemicals
Université Clermont Auvergne
2016-2025
Inserm
2015-2024
Centre National de la Recherche Scientifique
2015-2024
University of Tehran
2024
Royan Institute
2024
Academic Center for Education, Culture and Research
2024
Genetique Reproduction and Developpement
2013-2023
Tokushima University
2010
Saint Petersburg State Pediatric Medical University
2010
University of Calgary
1992-1998
The mammalian epididymis provides sperm with an environment that promotes their maturation and protects them from external stresses. For example, it harbors array of antioxidants, including non-conventional glutathione peroxidase 5 (GPX5), to protect oxidative stress. To explore the role GPX5 in epididymis, we generated mice lack epididymal expression enzyme. Histological analyses Gpx5-/- epididymides cells revealed no obvious defects. Furthermore, there were apparent differences...
This study investigated the enzymatic function of two putative plant GPXs, GPXle1 from Lycopersicon esculentum and GPXha2 Helianthus annuus , which show sequence identities with mammalian phospholipid hydroperoxide glutathione peroxidase (PHGPX). Both purified recombinant proteins expressed in Escherichia coli PHGPX activity by reducing alkyl, fatty acid hydroperoxides but not hydrogen peroxide presence glutathione. Interestingly, both also reduce as well using thioredoxin substrate....
We have isolated vesicular structures from mouse epididymal fluid, referred to as epididymosomes. Epididymosomes a roughly spherical aspect and bilayer membrane, they are heterogeneous in size content. They originate the epithelium, notably caput region, emitted lumen by way of apocrine secretion. characterized their membranous lipid profiles cauda epididymidal fluid samples found that epididymosomes were particularly rich sphingomyelin (SM) arachidonic acid. The proportion SM increased...
Does a novel antioxidant formulation designed to restore redox balance within the male reproductive tract, reduce sperm DNA damage and increase pregnancy rates in mouse models of oxidative stress? Oral administration significantly reduced glutathione peroxidase 5 (GPX5), knockout mice restored near-normal levels subjected scrotal heat stress. Animal human studies have documented adverse effect on fertilization rates, embryo quality, miscarriage transfer de novo mutations offspring. Semen...
Severe acute syndrome coronavirus 2 can invade a variety of tissues, including the testis. Even though this virus is scarcely found in human semen polymerase chain reaction tests, autopsy studies confirm viral presence all testicular cell types, spermatozoa and spermatids.
Can a discriminant threshold be determined for human sperm DNA oxidation? A was found with 65.8% of 8-hydroxy-2'-deoxyguanosine (8-OHdG)-positive cells and mean intensity fluorescence (MIF) 552 arbitrary units. Oxidative stress is known to interfere quality fertilizing capacity. However, current practice does not include the routine determination oxidative damage in spermatozoa; optimized consensus protocols are lacking no thresholds normality have been established. Intra- inter-method...
Sperm cells are remarkably complex and highly specialized compared to somatic cells. Their function is deliver the oocyte paternal genomic blueprint along with a pool of proteins RNAs so new generation can begin. Reproductive success, including optimal embryonic development healthy offspring, greatly depends on integrity sperm chromatin structure. It now well documented that DNA damage in linked reproductive failures both natural assisted conception (Assisted Technologies [ART]). This...
Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility scientific publications. This even important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition WHO Laboratory Manual Examination Processing Human Semen, International Standard ISO 23162:2021. With these tools, we propose authors should be instructed to follow laboratory methods order publish...
Do all regions of the paternal genome within gamete display equivalent vulnerability to oxidative DNA damage?Oxidative damage is not randomly distributed in mature human spermatozoa but instead targeted, with particular chromosomes being especially vulnerable stress.Oxidative frequently encountered male infertility patients. Such lesions can influence incidence de novo mutations children, yet it remains be established whether sperm susceptibility attack by reactive oxygen species.Human...