A5031104979- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Tissue Engineering and Regenerative Medicine
- Animal Genetics and Reproduction
- Mesenchymal stem cell research
- Chromosomal and Genetic Variations
- Cardiac Ischemia and Reperfusion
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Muscle Physiology and Disorders
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Xenotransplantation and immune response
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Monoclonal and Polyclonal Antibodies Research
- Endometriosis Research and Treatment
- Pluripotent Stem Cells Research
- Transplantation: Methods and Outcomes
- Ovarian function and disorders
- Urologic and reproductive health conditions
- Genomic variations and chromosomal abnormalities
Polish Academy of Sciences
2016-2025
Institute of Human Genetics
2016-2025
Medical University of Warsaw
2018
Municipal Institute for Medical Research
2018
Hospital Del Mar
2018
Pomeranian Medical University
1996-2014
Poznan University of Medical Sciences
2014
Martin Luther University Halle-Wittenberg
2014
University Hospital Heidelberg
2013
Medical University of Lodz
2012
The genetic basis of nonobstructive azoospermia is unknown in the majority infertile men.We performed array comparative genomic hybridization testing blood samples obtained from 15 patients with azoospermia, and we mutation screening by means direct Sanger sequencing testis-expressed 11 gene (TEX11) open reading frame semen 289 384 controls.We identified a 99-kb hemizygous loss on chromosome Xq13.2 that involved three TEX11 exons. This loss, which was identical 2 predicts deletion 79 amino...
Aims Several experimental studies and the initial clinical experience have shown that autologous skeletal myoblast transplantation into area of post-infarction left ventricular injury results in an increase segmental contractile performance. This phase I trial was designed to assess feasibility safety performed via a percutaneous trans-coronary-venous approach patients with dysfunction.
Reduced sperm motility, defined as asthenozoospermia, is a frequent cause of male infertility, and mainly connected with the dysfunction mitochondria. The aim this study was to identify proteins, thereby metabolic pathways, responsible for using 2-DE MALDI-TOF MS, correlate results obtained those two mitochondrial tests: JC-1 MitoSox Red. test performed activity, Red check whether observed poor motility associated reactive oxygen species (ROS) production. To proteins strictly reduced men...
Myocardial infarction (MI) is one of the most frequent causes death in industrialized countries. Stem cells therapy seems to be very promising for regenerative medicine. Skeletal myoblasts transplantation into postinfarction scar has been shown effective failing heart but shows limitations such, e.g. cell retention and survival. We synthesized investigated superparamagnetic iron oxide nanoparticles (SPIONs) as an agent direct labeling, which can used stem imaging. High quality, monodisperse...
Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility scientific publications. This even important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition WHO Laboratory Manual Examination Processing Human Semen, International Standard ISO 23162:2021. With these tools, we propose authors should be instructed to follow laboratory methods order publish...
Cytokines have been important mediators of the immunity and can be involved in numerous processes male genital tract including acting as immunomodulatory elements within gonad. The aims this study were: 1) to detect pro- anti-inflammatory cytokine levels control group subgroups infertile men; 2) set up practical recommendations concerning determination for infertility diagnosis. Observations were performed a 82 men: healthy controls (n = 27) patients 55). was further subdivided into with:...
Abnormal standard semen characteristics and reduced sperm chromatin maturity can appear with increasing male age.However, the influence of paternal age on parameters is still controversial.Therefore, this study was designed to estimate not only conventional but also DNA integrity.This research carried out ejaculated cells obtained from men (n = 1124) aged ≥40 y <40 y.Our data revealed a decreased volume an increased percentage DFI (sperm fragmentation index) in older compared younger entire...
Ischemic heart disease, also known as coronary artery disease (CAD), poses a challenge for regenerative medicine. iPSC technology might lead to breakthrough due the possibility of directed cell differentiation delivering new powerful source human autologous cardiomyocytes. One factors supporting proper maturation is in vitro culture duration. In this study, primary skeletal muscle myoblasts were selected myogenic type reservoir genetic reprogramming. Skeletal have similar ontogeny...
Genetic causes that lead to spermatogenetic failure in patients with nonobstructive azoospermia (NOA) have not been yet completely established.
ABSTRACT: Human semen contains spermatozoa as well populations of round nonspermatozoal cells primarily consisting leukocytes. Activation white blood present in the seminal plasma during genital tract inflammation and cellular reactions against microbial agents may provoke a release variety products such cytokines reactive oxygen species. The aim this study was to evaluate whether panel selected (interleukin [IL]‐1β, IL‐6, IL‐8, tumor necrosis factor‐α [TNFα]) detectable male could be...
ABSTRACT: We have studied the activity of substances (superoxide dismutase [SOD], catalase [Cat], malonaldehyde, xanthine oxidase [XO], nitric oxide [NOx]) participating in oxidative stress. Seminal plasma samples 147 ejaculates obtained from normal and infertile males were examined. Activities SOD, Cat, XO measured chemiluminometrically while malonaldehydes NOx by spectrophotometer seminal samples. Ejaculates previously characterized according to World Health Organization andrological...
We have examined the effect of white blood cells (WBCs), various proinflammatory cytokines, or a combination two on peroxidation human sperm membrane lipids in vitro conditions. Six recombinant such as interleukin-1beta (IL-1beta), IL-6, IL-8, IL-12, IL-18, and tumor necrosis factor alpha (TNF-alpha), used singly combinations, were analyzed. WBCs isolated from whole heparinized using density gradient technique (Histopaque 1.077). Spermatozoa semen samples with normal parameters by both...
The aim of the study was to examine an in vitro effect three bacterial strains (Escherichia coli, Staphylococcus haemolyticus and Bacteroides ureolyticus) on ejaculated spermatozoa with reference sperm membrane integrity mitochondrial activity. carried out swim-up-separated from 12 normozoospermic volunteers. Sperm plasma stability evaluated by LIVE/DEAD Viability Kit merocyanine 540 test. Mitochondrial activity using JC-1 test as well NADH-dependent NBT assay. percentage dead cells...