A5073293436- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- Renal and related cancers
- Chromosomal and Genetic Variations
- Animal Genetics and Reproduction
- LGBTQ Health, Identity, and Policy
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Telomeres, Telomerase, and Senescence
- Genomic variations and chromosomal abnormalities
- Reproductive biology and impacts on aquatic species
- Sexual Differentiation and Disorders
- Tissue Engineering and Regenerative Medicine
- Genetics and Neurodevelopmental Disorders
- Genital Health and Disease
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cancer Diagnosis and Treatment
- Bee Products Chemical Analysis
CReATe Fertility Centre
2016-2025
University of Toronto
2015-2024
Women's College Hospital
2020
Wayne State University
2015
Mount Sinai Hospital
2004-2010
Sunnybrook Health Science Centre
2010
Health Sciences Centre
2010
Laboratory of Molecular Genetics
2009
Université Laval
2007
University of Utah
2002-2007
The objective of this study was to examine the relationship between DNA integrity and protamines in human sperm. One hundred forty-nine male infertility patients were included an Institutional Review Board-approved study. Sperm evaluated for fragmentation using Integrity Assay, a test equivalent sperm chromatin structure assay (SCSA). Additionally, nuclear proteins extracted protamine-1/protamine-2 ratio (P1/P2), protamine-1 (P1), protamine-2 (P2), total protamine concentrations evaluated....
Absence of required RNA elements in sperm correlates with infertility but can be overcome assisted reproductive technologies.
Gonadal failure, along with early pregnancy loss and perinatal death, may be an important filter that limits the propagation of harmful mutations in human population. We hypothesized men spermatogenic impairment, a disease unknown genetic architecture common cause male infertility, are enriched for rare deleterious compared to normal spermatogenesis. After assaying genomewide SNPs CNVs 323 Caucasian idiopathic impairment more than 1,100 controls, we estimate each autosomal deletion detected...
Mammalian zygotic development is initiated by sperm-mediated intracellular calcium oscillations, followed activation of metaphase II-arrested oocytes. Sperm postacrosomal WW binding protein (PAWP) fulfils the criteria set for an oocyte-activating factor inducing oocyte and being stored in perinuclear theca, sperm compartment whose content first released into cytoplasm during fertilization. However, proof that PAWP initiates mammalian relies on demonstration it acts upstream oscillations....
Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility scientific publications. This even important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition WHO Laboratory Manual Examination Processing Human Semen, International Standard ISO 23162:2021. With these tools, we propose authors should be instructed to follow laboratory methods order publish...
What are the medical, psychosocial and legal aspects of gestational surrogacy (GS), including pregnancy outcomes complications, in a large series?Meticulous multidisciplinary teamwork, involving input for both intended parent(s) (IP) carrier (GC), is critical to achieve successful GS program.Small case series have described rates 17-50% GS. There no psychological not been addressed most these studies. To our knowledge, this largest reported series.A retrospective cohort study was performed....
A diverse pool of RNAs remain encapsulated within the transcriptionally silent spermatozoon despite dramatic reduction in cellular and nuclear volume following cytoplasm/nucleoplasm expulsion. The impact this pronounced restructuring on distribution transcripts inside sperm essentially remains unknown. To define their compartmentalization, total RNA >100 nt was extracted from sonicated (SS) mouse spermatozoa detergent demembranated sucrose gradient fractionated (Cs/Tx) heads. Sperm...
Assessment of sperm DNA damage has been suggested as a negative predictor fertility potential. Multiple pathological factors acting at both the intra-testicular and post-testicular levels may contribute to damage. The relative contribution each these in an individual with high (>30%) is unclear. management patients elevated also challenging. purpose our retrospective study was evaluate clinical course over 30% assess effect non-specific (oral antioxidant) cause-specific treatments on quality...
Testicular spermatozoa are utilized to achieve pregnancy in couples with severe male factor infertility. Several studies suggest that aneuploidy rates elevated at the testicular level infertile patients compared ejaculates of normal controls. However, essential data regarding between ejaculated and same individuals is lacking. The purpose our study was compare post-testicular from persistently high sperm DNA damage. Ejaculates biopsies were obtained eight damage (>30%). Both samples analyzed...
ABSTRACT: The objective of this study was to determine the effects low‐level laser light exposure on motility spermatozoa and DNA damage. Thirty‐three semen samples were collected for routine analysis classified as normospermic, oligospermic, or asthenospermic. After performed, residual divided into treated control aliquots. Treated exposed a 30‐second infrared pulse 50 mW/cm 2 at 905 nm, wavelength thought increase light‐sensitive cytochrome c oxidase in mitochondrial electron transport...
In clinical intracytoplasmic sperm injection (ICSI), a motile must be immobilized before insertion into an oocyte. This paper aims to develop robotic system for automated tracking, orientation control, and immobilization of sperms ICSI applications.We adapt the probabilistic data association filter by adding head state variables robustly tracking estimating tail positions under interfering conditions. The also utilizes motorized rotational microscopy stage new visual servo control strategy...
Measuring cell motility and morphology is important for revealing their functional characteristics. This paper presents automation techniques that enable automated, non-invasive measurement of parameters single sperm. Compared to the status quo qualitative estimation sperm's manually, provide quantitative data embryologists select a sperm intracytoplasmic injection. An adapted joint probabilistic association filter was used multi-sperm tracking tackled challenges identifying sperms intersect...
Cryopreservation of testicular and epididymal spermatozoa is more challenging in comparison to ejaculated due lower sperm concentration motility, higher sensitivity cryoprotectants. Sperm vitrification without the use potentially toxic permeable cryoprotectants an attractive freezing alternative for spermatozoa, as well oligoasthenoteratozoospermia (OAT) samples. Our study a retrospective analysis outcomes IVF cycles involving total 70 testicular, 77 69 OAT samples vitrified closed...
First trimester (FTM) and term human umbilical cord-derived perivascular cells (HUCPVCs), which are rich sources of mesenchymal stem (MSCs), can give rise to Sertoli cell (SC)-like as well haploid germ (GC)-like in vitro using culture conditions that recapitulate the testicular niche. Gamete-like have been produced ex vivo pluripotent MSCs. However, production functional gametes from has yet be achieved. Three independent lines FTM HUCPVCs were cultured a novel 5-week step-wise...