A5006990205- Prenatal Screening and Diagnostics
- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Genomic variations and chromosomal abnormalities
- Reproductive Health and Technologies
- Pregnancy and Medication Impact
- Chromosomal and Genetic Variations
- Congenital Anomalies and Fetal Surgery
- Fetal and Pediatric Neurological Disorders
- Genetic Syndromes and Imprinting
- Parvovirus B19 Infection Studies
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Blood Coagulation and Thrombosis Mechanisms
- Sexual Differentiation and Disorders
- Maternal Mental Health During Pregnancy and Postpartum
- Cannabis and Cannabinoid Research
- BRCA gene mutations in cancer
- Gestational Trophoblastic Disease Studies
- Nausea and vomiting management
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
CReATe Fertility Centre
2017-2025
University of Toronto
2013-2025
Ottawa Fertility Centre
2023-2024
Saints Cyril and Methodius University of Skopje
2021
Macedonian Academy of Sciences and Arts
2011-2016
SickKids Foundation
2013-2015
Hospital for Sick Children
2013-2015
Mount Sinai Hospital
2014
Centro de Ingeniería Genética y Biotecnología
2013
University Clinic of Traumatology
2012
To assess whether embryonic DNA isolated from blastocyst culture conditioned medium (BCCM) combined with blastocoel fluid (BF) could be used for stage non-invasive preimplantation genetic testing chromosomal aneuploidy (non-invasive screening, NIPGS).
Background Although age-related loss of chromosome Y (LOY) in normal hematopoietic cells is a well-known phenomenon, the phenotypic consequences LOY have been elusive. However, has found association with smoking, shorter survival and higher risk cancer. It was suggested that blood could become predictive biomarker male carcinogenesis. Aims, Methods & Findings To investigate for development colorectal (CC) prostate cancers (PC), we analyzed DNA samples from peripheral 101 CC patients (mean...
Preimplantation genetic testing for aneuploidies (PGT-A) using trophectoderm (TE) biopsy samples is labour intensive, invasive, and subject to sampling bias. In this study, we report on the efficacy factors affecting accuracy of a technique pioneered minimally invasive preimplantation aneuploidy (miPGT-A). Our uses cell-free embryonic DNA (cfeDNA) in spent embryo culture medium (SEM) combined with blastocoel fluid (BF) increase amount assayable cfeDNA. We compared miPGT-A results (n = 145...
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Abstract Blastocyst mosaicism is increasingly detected due to advances in preimplantation genetic testing for aneuploidy (PGT-A). While some mosaic embryos result a live birth, there are reports of altered implantation potential. Various approaches needed better understand their developmental uniqueness, including transcriptomic profile. This study aimed profile the signatures and investigate how influences global gene expression. Utilizing novel method enabling simultaneous sequencing both...
Abstract Improved embryo prioritization is crucial in optimizing the results assisted reproduction, especially light of increasing utilization elective single transfers. Embryo currently based on morphological criteria and some cases incorporates preimplantation genetic testing for aneuploidy (PGT-A). Recent technological advances have enabled parallel genomic transcriptomic assessment a cell. Adding analysis to PGT-A holds promise better understanding early embryonic development...
Abstract Globozoospermia is a rare but severe teratozoospermia, characterized by ejaculates consisting completely of round-headed spermatozoa that lack an acrosome or, in partial globozoospermia, containing variable proportion (20.0-90.0%) acrosomeless spermatozoa. Men are affected with total globozoospermia infertile, and even the application intracytoplasmic sperm injection (ICSI) has met disappointingly low success rates. In humans, several case reports have demonstrated two or more...
Prostate cancer (PC) is the second leading cause of deaths in men. The effects androgens on prostatic tissue are mediated by androgen receptor (AR) gene. 5' end exon 1 AR gene includes a polymorphic CAG triplet repeat that numbers between 10 to 36 normal population. length repeats inversely related transactivation function There controversy over association short and PC. This retrospective case-control study evaluates possible effect prostate risk Macedonian males. A total 392 male subjects,...
Spontaneous abortion is a significant clinical problem of different etiologies. Certain thrombophilia gene mutations have been associated with an increased risk spontaneous abortion. Also, in folate-related genes can lead to abnormal chromosomal segregation during meiosis which the most common cause We developed multiplex single-base extension reaction assay that allows simultaneous analysis 10 thrombophilia- and (Factor V Leiden G1691A, Factor H1299R, II G20210A, XIII V34L, PAI-I -675...
Meiotic recombination is crucial for human genetic diversity and chromosome segregation accuracy. Understanding its variation across individuals the processes by which it goes awry are long-standing goals in genetics. Current approaches inferring landscapes rely either on population patterns of linkage disequilibrium (LD)—capturing a time-averaged view—or direct detection crossovers gametes or multigeneration pedigrees, limits data set scale availability. Here, we introduce an approach...
Background. Nausea and vomiting of pregnancy (NVP) is the most common medical condition pregnancy, affecting up to 85% expecting mothers. In USA, there no FDA-approved medication for treatment NVP. Objective. To identify primary concerns American women leading them contact Motherisk NVP Helpline characterize severity their symptoms therapy offered in order develop improved customized counseling them. Methods. We reviewed intake forms who called from 2008 2012. extracted state residence,...
Meiotic recombination is crucial for human genetic diversity and chromosome segregation accuracy. Understanding its variation across individuals the processes by which it goes awry are long-standing goals in genetics. Current approaches inferring landscapes either rely on population patterns of linkage disequilibrium (LD)-capturing a time-averaged view-or direct detection crossovers gametes or multi-generation pedigrees, limits dataset scale availability. Here, we introduce an approach...
The most significant and well characterized genetic risk factors for breast and/or ovarian cancer are germline mutations in the BRCA1 BRCA2 genes. gene strikingly increase risk, suggesting that polymorphisms these genes logical candidates seeking to identify low penetrance susceptibility alleles. aim of this study was initiate a screen BRCA1/2 order variants Republic Macedonia, evaluate association several single nucleotide (SNPs) with risk. In study, we included 100 patients invasive from...
Congenital bilateral absence of vas deferens (CBAVD) is the most common CFTR-related disorder (CFTR-RD) that explains about 1–2% male infertility cases. Controversial data have been published regarding involvement CFTR mutations in infertile men with non-obstructive azoospermia and oligozoospermia. Here, we describe single base extension (SNaPshot) assay for detection 11 mutations: F508del, G542X, N1303K, 621+1G->T, G551D, R553X, R1162X, W1282X, R117H, 2184insA 1717-1G->A IVS8polyT variants....
The aim of this study was to determine whether cell free fetal (cff) DNA in residual amniotic fluid (AF) supernatant obtained from bloody, low-volume and late gestation samples can be used for prenatal diagnosis by quantitative fluorescence polymerase chain reaction (QF-PCR) array comparative genomic hybridization (aCGH).A total 49 compromised AFs were analyzed case-control, double-blinded study. processed through: a conventional cytogenetic approach utilizing Fluorescence situ Hybridization...