A5062064284- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- Renal and related cancers
- Sexual Differentiation and Disorders
- Testicular diseases and treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive System and Pregnancy
- Genomic variations and chromosomal abnormalities
- Pluripotent Stem Cells Research
- Ectopic Pregnancy Diagnosis and Management
- Cancer Genomics and Diagnostics
- Gestational Trophoblastic Disease Studies
- Thyroid Disorders and Treatments
- Male Reproductive Health Studies
- Endometriosis Research and Treatment
- Urologic and reproductive health conditions
- Sexual function and dysfunction studies
- Birth, Development, and Health
- Urological Disorders and Treatments
- Genetic Syndromes and Imprinting
- Animal Genetics and Reproduction
Saint Camillus International University of Health and Medical Sciences
2020-2025
Clinica Valle Giulia
2003-2025
Centro Medicina della Riproduzione
2021-2024
University of Teramo
2023
European Hospital
2013-2022
GynePro Medical
2019
Sapienza University of Rome
2002
Università Cattolica del Sacro Cuore
1999
Universidad de Granada
1999
Centre d'études de l'emploi
1991
Sperm DNA fragmentation is known to compromise male fertility. Previous findings have suggested the implication of oxidative stress in etiology this pathological condition. The present study was conducted find out if pathologically increased incidence ejaculated spermatozoa can be reduced by oral treatment with two antioxidants, vitamins C and E. Sixty-four men unexplained infertility an elevated (> or = 15%) percentage DNA-fragmented ejaculate were randomized between antioxidant (1 g...
BACKGROUND: It is known that repeated failure of assisted reproduction treatment (ART) can be due to a paternal effect. This study was undertaken analyse the possible relationship between ART and sperm DNA fragmentation. METHODS: Zygote morphology percentage spermatozoa with fragmented (assessed by TUNEL) were compared in two groups using donor oocytes for ICSI attempts. The experimental group consisted 18 infertile couples who had each undergone three previous failed control included...
Sperm DNA damage (fragmentation) is a recently discovered cause of male infertility for which no efficient treatment has yet been found. Previous findings have suggested that clinically relevant sperm may occur at the post-testicular level. This study was undertaken to assess clinical usefulness ICSI with testicular spermatozoa in this indication.The percentage fragmented DNA, assessed by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling assay, and outcomes were...
Are there correlations among human blastocyst ploidy status, standard morphology evaluation and time-lapse kinetics?Correlations were observed, in that euploid blastocysts showed a higher percentage with top quality inner cell mass (ICM) trophectoderm (TE), expansion grades shorter time to start of blastulation, hatching, compared aneuploid ones.Embryo has always been considered an important predictor successful implantation pregnancy. Nevertheless, knowledge the relative impact each...
Can next-generation sequencing (NGS) techniques be used reliably for comprehensive aneuploidy screening of human embryos from patients undergoing IVF treatments, with the purpose identifying and selecting chromosomally normal transfer?Extensive application NGS in clinical preimplantation genetic (PGS) cycles demonstrates that this methodology is reliable, allowing identification transfer euploid resulting ongoing pregnancies.The effectiveness PGS dependent upon biology early embryo...
BACKGROUND: Most studies examining the use of ICSI for cases elevated sperm DNA fragmentation report poor pregnancy and implantation rates. with testicular samples has recently been suggested these cases. Here we test a less invasive approach based on oral antioxidant treatment prior to ejaculated spermatozoa. METHODS: Thirty-eight men an (≥15%) percentage DNA-fragmented spermatozoa in ejaculate were treated antioxidants (1 g vitamin C 1 E daily) 2 months after one failed attempt. In 29...
BACKGROUND: Concentrations of certain substances in follicular fluid (FF) are related to fertilization outcome and early post-fertilization development. The study aim was identify FF markers with which predict embryo implantation potential. METHODS: selected hormones, cytokines growth factors individual samples obtained during assisted reproduction treatment were outcomes. RESULTS: Mean concentrations LH, hormone (GH), prolactin (PRL), 17β-estradiol (E2) insulin-like factor (IGF)-I higher,...
The aim of this study was to examine the relationship between different preincubation periods oocytes and outcome intracytoplasmic sperm injection (ICSI). We analysed retrospectively 95 ICSI treatment cycles performed alleviate severe male-factor infertility. Oocyte collection approximately 36 h after human chorionic gonadotrophin administration. cumulus-corona-oocyte complexes obtained were incubated until moment ICSI. Fertilization, embryo development implantation rates in four groups,...
Of 615 patients with impotence of varying etiologies who were followed from 12 to 96 months after the institution intracavernous self-injection therapy vasoactive drugs (papaverine alone, papaverine and alpha-blockers, Ceritine, a new multilevel acting drug) 87% (533 patients) returned for followup visits or regularly contacted. these sexual activity was restored in 91%. The dropout rate 11.25%. 114 episodes prolonged erections among 51 (4.57%) represented less than 3 per 1,000 34,875...
Germ cell apoptosis was evaluated in 11 men suffering from nonobstructive azoospermia and enrolled a spermatid conception programme. In six of these patients, round spermatids (Sa stage) were the most advanced spermatogenic cells recovered testicular biopsy samples. This condition is referred to as complete spermiogenesis failure. remaining five men, few late elongated (Sd unexpectedly found samples on day treatment. incomplete both groups patients examined by analysing smears prepared...
In spite of the availability abundant data about in vitro spermatogenesis laboratory animals, studies on human are scarce. This study employed a relatively simple culture system, involving all cell types seminiferous tubules, to analyze effects FSH and testosterone (T) different characteristics germ Sertoli cells culture. By using fluorescence in-situ hybridization, we show that reduction ploidy can be stimulated by but not T. FSH, T, also induced unexpectedly rapid (24–48 h) morphological...
Measurement of reactive oxygen species (ROS) producing leukocytes in semen has been a standard component the analysis, but its true significance remains still unknown. In this study, we have correlated number seminal to various parameters. We found negative correlation between leukocyte and sperm concentration (rs = -0.22; p 0.01) motility -0.20; 0.02). contrast, positive both ROS 0.70, < 0.001; n 125) spermatozoa with DNA fragmentation 0.43, 0.032; 25) was found. However, only trend...