A5045463023- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Ovarian function and disorders
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Reproductive Health and Technologies
- Gene expression and cancer classification
- RNA Research and Splicing
- Assisted Reproductive Technology and Twin Pregnancy
- Chromosomal and Genetic Variations
- Effects and risks of endocrine disrupting chemicals
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Bioinformatics and Genomic Networks
- Microbial metabolism and enzyme function
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Prenatal Screening and Diagnostics
- Birth, Development, and Health
- RNA modifications and cancer
- Protease and Inhibitor Mechanisms
Wayne State University
2016-2025
Michigan United
2012-2024
Prisma Health
2022
University of South Carolina
2022
Northwestern University
2022
Augusta University
2014-2022
Colorado School of Public Health
2022
University of Oklahoma Health Sciences Center
2022
University of Illinois Chicago
2022
University of Colorado Denver
2015-2017
Clomiphene is the current first-line infertility treatment in women with polycystic ovary syndrome, but aromatase inhibitors, including letrozole, might result better pregnancy outcomes.
There has been substantial interest in assessing whether RNAs (mRNAs and sncRNAs, i.e. small non-coding) delivered from mammalian spermatozoa play a functional role early embryo development. While the cadre of spermatozoal mRNAs characterized, comparatively little is known about distribution or function estimated 24,000 sncRNAs within each normal human spermatozoon.RNAs <200 bases length were isolated ejaculates three donors proved fertility. 18-30 nucleotides then used to construct RNA...
Polycystic ovary syndrome (PCOS) is a common, highly heritable complex disorder of unknown aetiology characterized by hyperandrogenism, chronic anovulation and defects in glucose homeostasis. Increased luteinizing hormone relative to follicle-stimulating secretion, insulin resistance developmental exposure androgens are hypothesized play causal role PCOS. Here we map common genetic susceptibility loci European ancestry women for the National Institutes Health PCOS phenotype, which confers...
Increasing attention has focused on the significance of RNA in sperm, light its contribution to birth and long-term health a child, role sperm function diagnostic potential. As composition is flux, assigning specific roles individual RNAs presents significant challenge. For first time RNA-seq was used characterize population coding non-coding transcripts human sperm. Examining representation as multiple methods library preparation revealed unique features indicative very stage-dependent...
During the haploid phase of mammalian spermatogenesis, nucleosomal chromatin is ultimately repackaged by small, highly basic protamines to generate an extremely compact, toroidal architecture that critical normal spermatozoal function. In common with several species, however, human spermatozoon retains a small proportion its packaged in nucleosomes. As spermatozoa structurally more open than protamine-packaged chromatin, we considered it likely be accessible exogenously applied...
The standard therapy for women with unexplained infertility is gonadotropin or clomiphene citrate. Ovarian stimulation letrozole has been proposed to reduce multiple gestations while maintaining live birth rates.We enrolled couples in a multicenter, randomized trial. Ovulatory 18 40 years of age at least one patent fallopian tube were randomly assigned ovarian (up four cycles) (301 women), (300), (299). primary outcome was the rate among clinical pregnancies.After treatment gonadotropin,...
Women with obesity and infertility are counseled to lose weight prior conception treatment improve pregnancy rates birth outcomes, although confirmatory evidence from randomized trials is lacking. We assessed whether a preconception intensive lifestyle intervention acute loss superior neutral at achieving healthy live birth.In this open-label, controlled study (FIT-PLESE), 379 women (BMI ≥ 30 kg/m2) unexplained were randomly assigned in 1:1 ratio 2 modification groups lasting 16 weeks,...
The packaging of the male haploid genome within differentiating spermatid nucleus is facilitated by small basic nuclear proteins called protamines. Although majority DNA in human sperm chromatin bound these proteins, a percentage retains nucleosomal-like component. These histone-enriched regions may possess enhanced nuclease sensitivity and have been postulated to designate certain genes involved early embryogenesis. We shown previously that domain containing two protamines PRM1 PRM2...
We are coming to appreciate that at fertilization human spermatozoa deliver the paternal genome alongside a suite of structures, proteins and RNAs. Although role some structures as requisite elements for early development has been established, function sperm-delivered RNAs remains point discussion. The presence in transcriptionally quiescent can only be derived from transcription precedes late spermiogenesis. A cross-platform microarray strategy was used assess profile spermatozoal...
We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These derived throughout spermatogenesis, representing record past events. Recently, evidence has been provided micro-RNAs present in testes, suggesting they might also be carried ejaculate spermatozoa. To directly test this hypothesis, unique microarray system capable identifying antisense and predicted transcripts was utilized. RNA isolated from spermatozoa 6 normal...
Context: There is no standardized assay of testosterone in women. Liquid chromatography mass spectrometry (LC/MS) has been proposed as the preferable by an Endocrine Society Position Statement.
The biological significance of nuclear scaffold/matrix-attachment regions (S/MARs) remains a topic long-standing interest. key to understanding S/MAR behavior relies on determining the physical attributes in vivo S/MARs and whether they serve as rigid or flexible chromatin loop anchors. To analyze behavior, single multiple copies S/MAR-containing constructs were introduced into various host genomes transgenic mice transfected cell lines. These integration events provided system study...