A5045878389- RNA Research and Splicing
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- Microtubule and mitosis dynamics
- Animal Genetics and Reproduction
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Silk-based biomaterials and applications
- Invertebrate Immune Response Mechanisms
- Chromosomal and Genetic Variations
- Herpesvirus Infections and Treatments
- Cellular Mechanics and Interactions
- Genetics and Neurodevelopmental Disorders
- Marine Ecology and Invasive Species
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Liver Disease Diagnosis and Treatment
- Heat shock proteins research
- Insect and Arachnid Ecology and Behavior
- Signaling Pathways in Disease
Princeton University
2016-2025
Institut de Biologie Intégrative de la Cellule
2016
George Mason University
2016
Hunter Holmes McGuire VA Medical Center
2016
Virginia Commonwealth University
2016
University of Washington
2011
Massachusetts Institute of Technology
1992-1996
Whitehead Institute for Biomedical Research
1992-1996
Howard Hughes Medical Institute
1992-1995
Stanford University
1988-1991
Ribosomes can read through stop codons in a regulated manner, elongating rather than terminating the nascent peptide. Stop codon readthrough is essential to diverse viruses, and phylogenetically predicted occur few hundred genes Drosophila melanogaster, but importance of eukaryotes remains largely unexplored. Here, we present ribosome profiling assay (deep sequencing ribosome-protected mRNA fragments) for provide first genome-wide experimental analysis readthrough. Readthrough far more...
ABSTRACT The site of oskar RNA and protein localization within the oocyte determines where in embryo primordial germ cells form abdomen develops. Initiation requires activity several genes. We show that ovaries mutant for any these genes lack Oskar protein. Using various transgenic constructs we have determined sequences required translational repression map to 3′UTR, while involved correct temporal activation translation reside outside 3′UTR. Upon at posterior pole, is maintain local-...
When Xenopus laevis oocyte nuclei are injected with a recombinant plasmid containing the Herpes Simplex Virus (HSV) thymidine kinase (tk) gene, 100- fold increase in tk enzymatic activity is observed.Three lines of evidence show that this result expression HSV gene.First, selectively inactivated by IgG fraction antiserum raised against protein.Second, polypeptide comigrates authentic on polyacrylamide gels synthesized uniquely oocytes gene.Third, induced found capable phosphorylating...
Abstract Patterning of the anterior-posterior body axis during Drosophila development depends on restriction Nanos protein to posterior early embryo. Synthesis occurs only when maternally provided nanos RNA is localized pole by a large, cis-acting signal in 3′ untranslated region (3′UTR); translation unlocalized repressed 90 nucleotide Translational Control Element (TCE), also 3′UTR. We now show quantitatively that majority embryo not but distributed throughout cytoplasm, indicating...
ABSTRACT Correct formation of the Drosophila body plan requires restriction nanos activity to posterior embryo. Spatial regulation is achieved by a combination RNA localization and localization-dependent translation such that only posteriorly localized translated. Cis-acting sequences mediate both translational lie within 3′ untranslated region. We have identified discrete control element region acts independently signal repression unlocalized RNA. Both regulatory function 3′UTR sequence are...
The cluster of homeotic genes known as the bithorax complex (BX-C) (Lewis 1978) is divisible into three complementation groups, or functional domains (Sánchez-Herrero et al. 1985). Each domain provides major developmental determinants for segment identity in one adjacent metameric regions that together extend from anterior/posterior compartment boundary (a/p) second thoracic (T2) eighth abdominal (A8) (Fig. 1). Thus, Ultrabithorax (Ubx) controls T2p-Ala region 1963, 1978, 1981, 1982; Morata...
Partitioning of mRNAs into ribonucleoprotein (RNP) granules supports diverse regulatory programs within the crowded cytoplasm. At least two types RNP populate germ plasm, a cytoplasmic domain at posterior Drosophila oocyte and embryo. Germ deliver required for germline development to pole cells, cell progenitors. A second type granule, here named founder granules, contains oskar mRNA, which encodes plasm organizer. Whereas mRNA is essential assembly during oogenesis, we show that it toxic...