A5029255990- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Nuclear Structure and Function
- Protist diversity and phylogeny
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Advanced Fluorescence Microscopy Techniques
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- Neurogenetic and Muscular Disorders Research
- Plant Taxonomy and Phylogenetics
- Parasitic Infections and Diagnostics
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
Department of Embryology
2012-2021
Carnegie Institution for Science
2012-2021
Johns Hopkins University
2014-2019
Carnegie Observatories
1988-2007
University of Missouri
2006
Rockefeller University
2002
Sorbonne Université
1993-1995
The Gurdon Institute
1992
Northwestern University
1964-1989
Fred Hutch Cancer Center
1983-1989
A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells a conventional tissue squash are immobilized under thin layer agar. Next they treated with alkali to denature then incubated tritium-labeled RNA. The detected by autoradiography. illustrated hybridization ribosomal amplified genes oocytes toad Xenopus. low level gene amplification was also premeiotic nuclei (oogonia).
Hybridization of radioactive nucleic acids with the DNA cytological preparations shows that sequences mouse satellite are located in centromeric heterochromatin chromosomes. Other types do not contain DNA.
We have identified a double-stranded (ds)RNA-binding domain in each of two proteins: the product Drosophila gene staufen, which is required for localization maternal mRNAs, and protein unknown function, Xlrbpa, from Xenopus. The amino acid sequences binding domains are similar to other additional protein. Database searches several proteins known or thought bind dsRNA, including human dsRNA-activated inhibitor (DAI), trans-activating region (TAR)-binding protein, Escherichia coli RNase III....
A method is presented for detecting the cellular location of specific DNA fractions. The technique involves hybridization a radioactive test in solution to stationary cytological preparation. Sites binding are then detected by autoradiography. Experiments with toad Xenopus described.
Gene expression variation is shaped by both genetic and environmental effects, yet these two factors are rarely considered together in the context of adaptive evolution. We studied influences on gene regulatory evolution ...Changes thought to play a major role While it known that highly sensitive environment, very few studies have determined influence effects ...
We report very high gene targeting frequencies in Drosophila by direct embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted replacement homologous recombination (HR) have been achieved up to 10% all targets at a given locus. In embryos that are wild type for DNA repair, the products dominated NHEJ mutations. recipients deficient component, ligase IV, majority arise HR with coinjected donor DNA, no...
Laser ablation MC-ICP-MS allows in situ strontium isotope data to be obtained for incrementally formed bioapatites such as enamel with extremely high spatial resolution. Here, we provide a large-scale application of the method comparing ...Understanding mobility and landscape use is important reconstructing subsistence behavior, range, group size, it may contribute our understanding phenomena dynamics biological cultural interactions between distinct ...
Significance A hexanucleotide repeat expansion in the first intron of C9orf72 gene represents most prominent form heritable amyotrophic lateral sclerosis. Bidirectional transcription and ATG-independent translation expanded (GGGGCC) n specifies production toxic glycine:arginine (GR ) proline:arginine (PR poly-dipeptides. The present study provides evidence that PR poly-dipeptide binds directly to central channel nuclear pores, causing inhibition both import export macromolecules from...
We have examined the distribution of RNA transcription and processing factors in amphibian oocyte nucleus or germinal vesicle. polymerase I (pol I), pol II, III occur Cajal bodies (coiled bodies) along with various components required for three classes nuclear transcripts: mRNA, rRNA, transcripts. Among these are factor IIF (TFIIF), TFIIS, splicing factors, U7 small ribonucleoprotein particle, stem-loop binding protein, SR proteins, cleavage polyadenylation nucleolar RNAs, proteins that...
Nuclear organelles, unlike many cytoplasmic lack investing membranes and are thus in direct contact with the surrounding nucleoplasm. Because properties of nucleoplasm nuclear organelles influence exchange molecules from one compartment to another, it is important understand their physical structure. We studied density permeability nucleoli, Cajal bodies (CBs), speckles Xenopus oocyte nucleus or germinal vesicle (GV). Refractive indices were measured by interferometry within intact GVs...
Immediately after the initiation of transcription in eukaryotes, nascent RNA polymerase II transcripts are bound by nuclear proteins resulting formation heterogeneous ribonucleoprotein (hnRNP) complexes. hnRNP complexes from HeLa cell nuclei contain greater than 20 major molecular mass range 34,000-120,000 D. Among these previously described A, B, and C groups (34,000-43,000 D) several larger, as yet uncharacterized, proteins. Here we describe isolation characterization a novel protein...
This paper describes the replication of centrioles during spermatogenesis in Prosobranch snail, Viviparus malleatus Reeve. Sections for electron microscopy were cut from pieces testis fixed OsO(4) and embedded polyester resin Vestopal W. Two kinds spermatocytes are present. These give rise to typical uniflagellate sperm carrying haploid number 9 chromosomes, atypical multiflagellate with only one chromosome. present youngest spermatocyte. Each is a hollow cylinder about 160 mmicro diameter...