A5065911398- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Protein Structure and Dynamics
- Advanced Fluorescence Microscopy Techniques
- Cancer-related gene regulation
- Machine Learning in Bioinformatics
- Gene expression and cancer classification
- Modular Robots and Swarm Intelligence
- Heat shock proteins research
- RNA regulation and disease
- Animal Genetics and Reproduction
- Fungal and yeast genetics research
- ATP Synthase and ATPases Research
- Microbial Metabolites in Food Biotechnology
- RNA Interference and Gene Delivery
- Plant Molecular Biology Research
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
Johns Hopkins University
2019-2024
New York University
2015-2020
Howard Hughes Medical Institute
2016-2018
Albert Einstein College of Medicine
2006-2013
The Bronx Defenders
2006
Abstract Germ granules, specialized ribonucleoprotein particles, are a hallmark of all germ cells. In Drosophila , an estimated 200 mRNAs enriched in the plasm, and some these have important, often conserved roles cell formation, specification, survival migration. How spatially distributed within granule whether their position defines functional properties is unclear. Here we show, using single-molecule FISH structured illumination microscopy, super-resolution approach, that organized...
Nonsense-mediated mRNA decay (NMD) is a quality control mechanism responsible for “surveying” mRNAs during translation and degrading those that harbor premature termination codon (PTC). Currently the intracellular spatial location of NMD kinetics its step in mammalian cells are under debate. To address these issues, we used single-RNA fluorescent situ hybridization (FISH) measured PTC-containing β-globin intact single after induction gene transcription. This approach preserves temporal...
Germ granules are non-membranous ribonucleoprotein deemed the hubs for post-transcriptional gene regulation and functionally linked to germ cell fate across species. Little is known about physical properties of how these relate function. Here we study two types in Drosophila embryo: cytoplasmic that instruct primordial cells (PGCs) formation nuclear within early PGCs with unknown We show phase transitioned condensates nucleated by Oskar protein display liquid as well hydrogel-like...
We have been able to observe the dynamic interactions between a specific messenger RNA (mRNA) and its protein product in vivo by studying synthesis assembly of peripherin intermediate filaments (IFs). The results show that mRNA-containing particles (messenger ribonucleoproteins [mRNPs]) move mainly along microtubules (MT). These mRNPs are translationally silent, initiating translation when they cease moving. Many contain multiple mRNAs, possibly amplifying total amount synthesized within...
Localization of mRNA is a critical mechanism used by large fraction transcripts to restrict its translation specific cellular regions. Although current high-resolution imaging techniques provide ample information, the analysis methods for localization have either been qualitative or employed quantification in nonrandomly selected regions interest. Here, we describe an analytical method objective using combination two characteristics molecular distribution, polarization and dispersion. The...
RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which form under intense investigation. Here, we characterize the role of mRNAs and proteins in formation germ Drosophila. Super-resolution microscopy reveals number, size, distribution is precisely controlled. Surprisingly, granule not required for nucleation or persistence but instead control their size composition. Using an RNAi screen, determine regulators, helicases,...
Abstract Many eukaryotic proteins contain intrinsically disordered regions (IDRs) that intersperse globular folded domains, in contrast with bacterial which are typically highly 1, 2 . Recent years have seen great progress identifying biological functions associated these elusive protein sequence: specific cases, they mediate liquid phase separation 3 , perform molecular recognition 4 or act as sensors to changes the environment 5 Nevertheless, only a small number of IDRs annotated 6 despite...
Drosophila germ granules enrich mRNAs critical for fly development. Within granules, form multi-transcript clusters marked by increased mRNA concentration, creating an elevated potential intermolecular base pairing. However, the type and abundance of pairing in is poorly characterized. Using single-molecule super-resolution microscopy, chemical probing accessibility, phase separation assays, simulations, we demonstrated that remain well-folded upon localization to granules. While most...
mRNAs enriched in membraneless condensates provide functional compartmentalization within cells.How sort and organize these is poorly understood.Here, we report on a self-sorting mechanism by which multiple derived from the same gene assemble into homotypic mRNA clusters.In Drosophila germ granules, clusters formed originating different genes are de-mixed each other granule located at distinct positions homogeneously-distributed protein environment of granule.The specificity for not...
ABSTRACT Germ granules are non-membranous ribonucleoprotein deemed the hubs for post-transcriptional gene regulation and functionally linked to germ cell fate across species. Little is known about physical properties of how these relate function. Here we study two types in Drosophila embryo: cytoplasmic that instruct primordial cells (PGCs) formation nuclear within early PGCs with unknown We show phase transitioned condensates nucleated by Oskar protein display liquid as well hydrogel-like...