A5077272709- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Renal and related cancers
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
Johns Hopkins University
2020-2024
Emory National Primate Research Center
2021
To demonstrate that functional spermatids can be derived in vitro from nonhuman primate pluripotent stem cells.Green fluorescent protein-labeled, rhesus macaque embryonic cells (nhpESCs) were differentiated into advanced male germ cell lineages using a modified serum-free spermatogonial culture medium. In vitro-derived round spermatid-like (rSLCs) nhpESCs assessed for their ability to fertilize oocytes by intracytoplasmic sperm(atid) injection.Multiple academic laboratory settings.Not...
Drosophila germ granules enrich mRNAs critical for fly development. Within granules, form multi-transcript clusters marked by increased mRNA concentration, creating an elevated potential intermolecular base pairing. However, the type and abundance of pairing in is poorly characterized. Using single-molecule super-resolution microscopy, chemical probing accessibility, phase separation assays, simulations, we demonstrated that remain well-folded upon localization to granules. While most...