A5004487444- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Molecular Biology Techniques and Applications
- Electrostatics and Colloid Interactions
- Genomics and Chromatin Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Nanopore and Nanochannel Transport Studies
- Gene Regulatory Network Analysis
- Memory and Neural Mechanisms
- RNA modifications and cancer
- Obsessive-Compulsive Spectrum Disorders
- Neuroendocrine regulation and behavior
- Diffusion and Search Dynamics
- Protein Structure and Dynamics
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
Seoul National University
2015-2024
University of Minnesota
2022-2024
National University
2024
National Institute of Biological Resources
2022
Chung-Ang University
2019
Ewha Womans University
2017
Yonsei University
2017
Albert Einstein College of Medicine
2010-2015
Helix (United States)
2015
Howard Hughes Medical Institute
2015
The transcription and transport of messenger RNA (mRNA) are critical steps in regulating the spatial temporal components gene expression, but it has not been possible to observe dynamics endogenous mRNA primary mammalian tissues. We have developed a transgenic mouse which all β-actin is fluorescently labeled. found that fibroblasts localizes predominantly by diffusion trapping as single mRNAs. In cultured neurons acute brain slices, we multiple mRNAs can assemble together, travel active...
Abstract Astrocytes can affect animal behavior by regulating tripartite synaptic transmission, yet their influence on affective remains largely unclear. Here we showed that hippocampal astrocyte calcium activity reflects mouse state during virtual elevated plus maze test using two-photon imaging in vivo. Furthermore, optogenetic activation elevating intracellular induced anxiolytic behaviors astrocyte-specific channelrhodopsin 2 (ChR2) transgenic mice (hGFAP-ChR2 mice). As underlying...
Directed cell motility is at the basis of biological phenomena such as development, wound healing, and metastasis. It has been shown that substrate attachments mediate by coupling cell's cytoskeleton with force generation. However, it unclear how persistence directionality facilitated. We show mRNA localization plays an important role in this process, but mechanism action still unknown. In study, we zipcode-binding protein 1 transports β-actin to focal adhesion compartment, where dwells for...
Localization of messenger ribonucleoproteins (mRNPs) plays an essential role in the regulation gene expression for long-term memory formation and neuronal development. Knowledge concerning nature mRNP transport is thus crucial understanding how mRNPs are delivered to their target synapses. Here, we report experimental theoretical evidence that active dynamics mRNPs, which distinct from previously reported motor-driven transport, follows aging Lévy walk. Such nonergodic, transient...
As basic units of neural networks, ensembles synapses underlie cognitive functions such as learning and memory. These synaptic engrams show elevated density among engram cells following contextual fear memory formation. Subsequent analysis the CA3-CA1 synapse revealed larger spine sizes, connectivity correlated with strength. Here, we elucidate dynamics between CA3 CA1 by tracking identical at multiple time points adapting two-photon microscopy dual-eGRASP technique in vivo. After formation,...
We describe a microfluidic mixer that is well-suited for kinetic studies of macromolecular conformational change under broad range experimental conditions. The exploits hydrodynamic focusing to create thin jet containing the macromolecules interest. Kinetic reactions are triggered by molecular diffusion into from adjacent flow layers. ultimate time resolution these devices can be restricted premature contact between co-flowing solutions during process. Here, we design and characterization in...
Can nonspecifically bound divalent counterions induce attraction between DNA strands? Here, we present experimental evidence demonstrating short strands mediated by ${\mathrm{Mg}}^{2+}$ ions. Solution small angle x-ray scattering data collected as a function of concentration enable model independent extraction the second virial coefficient. As [${\mathrm{Mg}}^{2+}$] increases, this coefficient turns from positive to negative reflecting transition repulsive attractive inter-DNA interaction....
A microfluidic mixer is applied to study the kinetics of calmodulin conformational changes upon Ca2+ binding. The device facilitates rapid, uniform mixing by decoupling hydrodynamic focusing from diffusive and accesses time scales tens microseconds. used in conjunction with multiphoton microscopy examine fast Ca2+-induced transitions acrylodan-labeled calmodulin. We find that kinetic rates two homologous globular domains differ more than an order magnitude. characteristic constants are...
Metastasis involves tumor cell detachment from the primary tumor, and acquisition of migratory invasive capabilities. These capabilities are mediated by multiple events, including loss cell–cell contact, an increase in focal adhesion turnover failure to maintain a normal polarity. We have previously reported that silencing expression zipcode-binding protein IMP1/ZBP1 breast patients is associated with metastasis. selectively binds group mRNAs encode important mediators for motility. Here, we...
A transgenic mouse reveals real-time dynamics of transcription and transport endogenous Arc mRNA in live neurons.
L1 retrotransposons can pose a threat to genome integrity. The host has evolved restrict replication. However, mechanisms underlying propagation out of the surveillance remains unclear. Here, we propose an evolutionary survival strategy L1, which exploits RNA m6A modification. We discover that 'writer' METTL3 facilitates retrotransposition, whereas 'eraser' ALKBH5 suppresses it. essential cluster is located on 5' UTR serves as docking site for eukaryotic initiation factor 3 (eIF3), enhances...
Abstract Biomolecular condensates, often assembled through phase transition mechanisms, play key roles in organizing diverse cellular activities. The material properties of ranging from liquid droplets to solid-like glasses or gels, are features impacting the way resident components associate with one another. However, it remains unclear whether and how different would influence specific functions condensates. Here, we combine optogenetic control separation single-molecule mRNA imaging study...
The competition of monovalent and divalent cations for proximity to negatively charged DNA is biological importance can provide strong constraints theoretical treatments polyelectrolytes. Resonant x-ray scattering experiments have allowed us monitor the number distribution each cation in a mixed ion cloud around DNA. These measurements experimental evidence support general prediction: normalized polyelectrolytes remains constant when ions are at different ratios. In addition, amplitudes...
The elements that we have identified in the 5'-flanking region of rat growth hormone gene are shown Figure 7, and following conclusions drawn: 1) Cell-specific expression is mediated by two CSEs, which located from -95 to -65 -137 -107.2) These CSEs bind a common cell-specific trans-acting factor (GH-CSF), found hormone-producing cells. 3) Enhanced levels may involve protein-protein interaction when this binds on same side DNA helix as CSEs. 4) A TRE between -208 -178. 5) Activation thyroid...
Localization of mRNA is a critical mechanism used by large fraction transcripts to restrict its translation specific cellular regions. Although current high-resolution imaging techniques provide ample information, the analysis methods for localization have either been qualitative or employed quantification in nonrandomly selected regions interest. Here, we describe an analytical method objective using combination two characteristics molecular distribution, polarization and dispersion. The...
While recent studies have explored the maintenance of effect meditation on stress resilience, underlying neural mechanisms not yet been investigated. The present study conducted a highly controlled residential 4-day intervention to investigate brain functional changes and long-term effects mindfulness resilience. Thirty participants in practice 17 relaxation retreat (control group) underwent magnetic resonance imaging scans at baseline post-intervention completed Cognitive Affective...