A5066282020- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Gut microbiota and health
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Oral microbiology and periodontitis research
- Vibrio bacteria research studies
- Salivary Gland Disorders and Functions
- Cell Image Analysis Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Biosensing Techniques and Applications
- Listeria monocytogenes in Food Safety
- Immune Response and Inflammation
- Advanced Electron Microscopy Techniques and Applications
- Bacteriophages and microbial interactions
- Image Processing Techniques and Applications
- Optical Imaging and Spectroscopy Techniques
- Force Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Enzyme Structure and Function
- Laser-Matter Interactions and Applications
- Cell Adhesion Molecules Research
- Photosynthetic Processes and Mechanisms
Cornell University
2016-2025
Saint Meinrad Seminary and School of Theology
2019
Ithaca College
2011-2013
Albert Einstein College of Medicine
2005-2012
Yeshiva University
2012
Rubin
2012
Albany State University
2011
University at Albany, State University of New York
2011
Dongguk University
2011
New York Proton Center
2010
The use of semiconductor nanocrystals (quantum dots) as fluorescent labels for multiphoton microscopy enables multicolor imaging in demanding biological environments such living tissue. We characterized water-soluble cadmium selenide-zinc sulfide quantum dots live animals. These probes have two-photon action cross sections high 47,000 Goeppert-Mayer units, by far the largest any label used microscopy. visualized dynamically through skin mice, capillaries hundreds micrometers deep. found no...
Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images the resolution detail standard histology without use exogenous stains. Imaging indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, indoleamines, collagen provides crucial information for physiology pathology. The efficient application multiphoton to imaging requires knowledge...
Intrinsic, three-dimensionally resolved, microscopic imaging of dynamical structures and biochemical processes in living preparations has been realized by nonlinear laser scanning fluorescence microscopy. The search for useful two-photon three-photon excitation spectra, motivated the emergence microscopy as a powerful biophysical instrument, now discovered virtual artist's palette chemical indicators, fluorescent markers, native biological fluorophores, including NADH, flavins, green...
We have found that two-photon fluorescence imaging of nicotinamide adenine dinucleotide (NADH) provides the sensitivity and spatial three-dimensional resolution to resolve metabolic signatures in processes astrocytes neurons deep highly scattering brain tissue slices. This functional reveals spatiotemporal partitioning glycolytic oxidative metabolism between during focal neural activity establishes a unifying hypothesis for neurometabolic coupling which early is eventually sustained by late...
Individual plastids of vascular plants have generally been considered to be discrete autonomous entities that do not directly communicate with each other. However, in transgenic which the plastid stroma was labeled green fluorescent protein (GFP), thin tubular projections emanated from individual and sometimes connected other plastids. Flow GFP between interconnected could observed when a single or an interconnecting tubule photobleached loss fluorescence by both seen. These tubules allow...
We study and demonstrate the technique of simultaneous spatial temporal focusing femtosecond pulses, with aim to improve signal-to-background ratio in multiphoton imaging. This concept is realized by spatially separating spectral components pulses into a "rainbow beam" recombining these only at focus objective lens. Thus, pulse width becomes function distance, shortest confined focus. developed analytical expressions describe this method experimentally demonstrated feasibility. The has great...
Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established 3PE cross section measurements tryptophan the monoamines dopamine, circumvents limitations imposed photodamage, scattering, indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along concentration (∼50 mM) content (up to ∼5 × 10 8 molecules) individual secretory granules.
To determine how to utilize the green fluorescent protein (GFP) as a marker for subcellular localization and label plant mitochondria in vivo, transgenic suspension cells tobacco plants expressing GFP with without mitochondrial signal were generated. The first form used, GFP1, is easily observable low autofluorescence, such or trichomes, but masked tissue. For visualization of tissues high leaf, use very strong promoter (35S35SAMV), highly expressed modified mGFP4 coding region brighter...
Abstract The arbuscular mycorrhizal (AM) symbiosis is responsible for huge fluxes of photosynthetically fixed carbon from plants to the soil. Carbon transferred plant fungus as hexose, but main form stored by mycobiont at all stages its life cycle triacylglycerol. Previous isotopic labeling experiments showed that exports this storage lipid intraradical mycelium (IRM) extraradical (ERM). Here, in vivo multiphoton microscopy was used observe movement bodies through fungal colony and determine...
The fabrication of fluidic channels with dimensions smaller than 1 μm is described and characterized in respect to their use for detection individual DNA molecules. sacrificial layer technique used fabricate these devices as it provides CMOS-compatible materials exhibiting low fluorescence background. It also allows creating microfluidics circuitry submicrometer great control. small facilitate single molecule minimize events simultaneous passage more one through the measurement volume....
Multiphoton fluorescence photobleaching recovery (MP-FPR) is a technique for measuring the three-dimensional (3D) mobility of fluorescent molecules with 3D spatial resolution few microns. A brief, intense flash mode-locked laser light pulses excites via multiphoton excitation in an ellipsoidal focal volume and photobleaches fraction. Because fluorophores intrinsically confined to high-intensity illuminating beam, bleached region restricted known, three-dimensionally defined volume....
Water-soluble quantum dots (qdots) are now being used in life sciences research to take advantage of their bright, easily excited fluorescence and high photostability. Although the frequent erratic blinking substantial dark (never radiant) fractions that occur all available qdots may interfere with many applications, these properties individual particles biological environments had not been fully evaluated. By labeling Qdot-streptavidin organic dyes, we were able distinguish bright observe...
The peripheral nervous system has complex and intricate ramifications throughout many target organ systems. To date this not been effectively labeled by genetic markers, due largely to inadequate transcriptional specification minimum promoter constructs. Here we describe transgenic mice in which enhanced green fluorescent protein (eGFP) is expressed under the control of endogenous choline acetyltransferase (ChAT) regulatory elements, knock-in eGFP within a bacterial artificial chromosome...
Many cartilaginous tissues such as intervertebral disc (IVD) display a heterogeneous collagen microstructure that results in mechanical anisotropy. These structures are responsible for function of the tissue and regulate cellular interactions metabolic responses cells embedded within these tissues. Using gels seeded with ovine annulus fibrosus cells, constructs varying structure heterogeneity were created to mimic circumferential alignment IVD. Alignment was induced by contracting annular...
The mechanism of the recently reported photocontrolled cationic polymerization vinyl ethers was investigated using a variety catalysts and chain-transfer agents (CTAs) as well diverse spectroscopic electrochemical analytical techniques. Our study revealed complex activation step characterized by one-electron oxidation CTA. This is followed mesolytic cleavage resulting radical cation species, which leads to generation reactive cation-this species initiates ether monomer-and dithiocarbamate...
Fifteen water-soluble rhenium compounds of the general formula [Re(CO)3(NN)(PR3)]+, where NN is a diimine ligand and PR3 1,3,5-triaza-7-phosphaadamantane (PTA), tris(hydroxymethyl)phosphine (THP), or 1,4-diacetyl-1,3,7-triaza-5-phosphabicylco[3.3.1]nonane (DAPTA), were synthesized characterized by multinuclear NMR spectroscopy, IR X-ray crystallography. The complexes bearing THP DAPTA ligands exhibit triplet-based luminescence in air-equilibrated aqueous solutions with quantum yields ranging...
Rapid growth and perivascular invasion are hallmarks of glioblastoma (GBM) that have been attributed to the presence cancer stem-like cells (CSCs) their association with niche. However, mechanisms by which niche regulates GBM CSCs remain poorly understood due in part a lack relevant model systems. To simulate conditions analyze consequential changes invasion, patient-derived spheroids were co-cultured brain endothelial (ECs) microfabricated collagen gels. Integrating these systems 3D imaging...