A5071630308- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Galectins and Cancer Biology
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Trace Elements in Health
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Amino Acid Enzymes and Metabolism
- Biomedical Ethics and Regulation
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Circular RNAs in diseases
- Microfluidic and Bio-sensing Technologies
- Mechanical stress and fatigue analysis
- Plant and Biological Electrophysiology Studies
- Photosynthetic Processes and Mechanisms
- Cancer Research and Treatments
- Nanopore and Nanochannel Transport Studies
- Bone Tissue Engineering Materials
- Plant responses to water stress
- T-cell and Retrovirus Studies
- Cell Image Analysis Techniques
Cornell University
2016-2024
University of Virginia
2022-2024
Broad Institute
2023
Institute of Photonic Sciences
2023
University of Tennessee at Knoxville
2023
John Wiley & Sons (United States)
2019
Hudson Institute
2019
The composition of the plasma membrane (PM) must be tightly controlled despite constant, rapid endocytosis, which requires active, selective recycling endocytosed components. For many proteins, mechanisms, pathways, and determinants this PM remain unknown. We report that association with ordered, lipid-driven microdomains (known as rafts) is sufficient for localization a subset transmembrane proteins abrogation raft disrupts their trafficking leads to degradation in lysosomes. Using...
The glycocalyx is a coating of protein and sugar on the surface all living cells. Dramatic perturbations to composition structure are frequently observed in aggressive cancers. However, tools experimentally mimic model cancer-specific remain limited. Here, we develop genetically encoded toolkit engineer chemical physical cellular glycocalyx. By manipulating structure, able switch adhesive state cells from strongly adherent fully detached. Surprisingly, find that thick dense with high...
Mesenchymal stromal cells (MSCs) can be used intra-articularly to quell inflammation and promote cartilage healing; however, mechanisms by which MSCs mitigate joint disease remain poorly understood. Galectins, a family of β-galactoside binding proteins, regulate inflammation, adhesion cell migration in diverse types. Galectin-1 galectin-3 are proposed important intra-articular modulators both osteoarthritis rheumatoid arthritis. Here, we asked whether equine bone marrow-derived (BMSCs)...
Abstract Lubricin is a mucinous, synovial fluid glycoprotein that enables near frictionless joint motion via adsorption to the surface of articular cartilage and its lubricating properties in solution. Extensive O- linked glycosylation within lubricin’s mucin-rich domain critical for boundary function; however, it unknown exactly how facilitates lubrication. Here, we find lubricin glycome enriched with terminal β-galactosides, known binding partners family multivalent lectins called...
Cells feel the tension of implanted and injected silicone materials.
The organelles of eukaryotic cells maintain distinct protein and lipid compositions required for their specific functions. mechanisms by which many these components are sorted to locations remain unknown. While some motifs mediating subcellular localization have been identified, membrane proteins most lipids lack known sorting determinants. A putative mechanism is based on domains as rafts, laterally segregated nanoscopic assemblies proteins. To assess the role such in secretory pathway, we...
ABSTRACT The cancer cell glycocalyx serves as a major line of defense against immune surveillance. However, how specific physical properties the contribute to evasion and these are regulated not well understood. Here, we uncover surface density, glycosylation, crosslinking cancer-associated mucins nanoscale material thickness glycocalyx, further analyze effect on resistance effector attack. Natural Killer (NK) cell-mediated cytotoxicity exhibits near perfect inverse correlation with target...
The organelles of eukaryotic cells maintain distinct protein and lipid compositions required for their specific functions. mechanisms by which many these components are sorted to locations remain unknown. While some motifs mediating subcellular localization have been identified, membrane proteins most lipids lack known sorting determinants. A putative mechanism is based on domains as rafts, laterally segregated nanoscopic assemblies proteins. To assess the role such in secretory pathway, we...
Widespread therapeutic and commercial interest in recombinant mucin technology has emerged due to the unique ability of glycoproteins hydrate, protect, lubricate biological surfaces. However, production large, highly repetitive domains that are characteristic mucins remains a challenge biomanufacturing likely due, at least part, inherent instability DNA repeats cellular genome. To overcome this challenge, we exploit codon redundancy encode desired polypeptides with minimal nucleotide...
ABSTRACT The organelles of eukaryotic cells maintain distinct protein and lipid compositions required for their specific functions. mechanisms by which many these components are sorted to locations remain unknown. While some motifs mediating subcellular localization have been identified, membrane proteins most lipids lack known sorting determinants. A putative mechanism is based on domains as rafts, laterally segregated nanoscopic assemblies proteins. To assess the role such in secretory...
Optimization of host-cell production systems with improved yield and reliability is desired to meet the increasing demand for biologics complex posttranslational modifications. Aggregation suspension-adapted mammalian cells remains a significant problem that can limit cellular density per volume bioreactors. Here, we propose genetically encoded technology directs synthesis antiadhesive protective coatings on surface. Inspired by natural ability mucin glycoproteins resist adhesion hydrate...
The organelles of eukaryotic cells maintain distinct protein and lipid compositions required for their specific functions. mechanisms by which many these components are sorted to locations remain unknown. While some motifs mediating subcellular localization have been identified, membrane proteins most lipids lack known sorting determinants. A putative mechanism is based on domains as rafts, laterally segregated nanoscopic assemblies proteins. To assess the role such in secretory pathway, we...
Abstract Muc1 is a large, heavily O-glycosylated membrane protein that overexpressed in the majority of cancers and constitutes major structural element cancer cell glycocalyx. While biophysical properties glycocalyx directly regulate cell-ECM adhesion, cell-cell interactions, receptor spatial organization, how these change dynamically during acquisition metastatic phenotype largely unknown. To dissect relationship among glycan biosynthesis, mucin rigidity, bulk cancer-specific glycocalyx,...
The organelles of eukaryotic cells maintain distinct protein and lipid compositions required for their specific functions. mechanisms by which many these components are sorted to locations remain unknown. While some motifs mediating subcellular localization have been identified, membrane proteins most lipids lack known sorting determinants. A putative mechanism is based on domains as rafts, laterally segregated nanoscopic assemblies proteins. To assess the role such in secretory pathway, we...
Abstract The cancer cell glycocalyx serves as a major line of defense against immune surveillance. However, how specific physical properties the are regulated and contribute to evasion is not well understood. Here, we uncover surface density, glycosylation, crosslinking cancer-associated mucins nanoscale material thickness glycocalyx, further analyze effect on resistance effector attack. Natural Killer (NK) cell-mediated cytotoxicity exhibits near perfect inverse correlation with target...
US$18141 (US and Rest of World), € 11707 (Europe), £ 9260 (UK
A comparison of human mesenchymal stem cell osteogenesis in poly(ethylene glycol)