A5065746465- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Biochemical and biochemical processes
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Catalysis for Biomass Conversion
- Viral Infectious Diseases and Gene Expression in Insects
- Fermentation and Sensory Analysis
- Analytical Chemistry and Chromatography
- Biosensors and Analytical Detection
- Algal biology and biofuel production
- Polymer Surface Interaction Studies
- Surface Modification and Superhydrophobicity
- Genetic and Kidney Cyst Diseases
- Sirtuins and Resveratrol in Medicine
- Dental materials and restorations
- Metabolism and Genetic Disorders
- Spectroscopy and Chemometric Analyses
- Plant biochemistry and biosynthesis
- Tea Polyphenols and Effects
- Microbial Metabolism and Applications
- Chemistry and Chemical Engineering
- Neonatal Health and Biochemistry
- Genetic Syndromes and Imprinting
University of Minho
2015-2024
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Institute for Biotechnology and Bioengineering
2011-2017
Stemmatters (Portugal)
2017
Hospital Maria Pia
1994-1997
Abstract BACKGROUND γ‐Decalactone production from ricinoleic acid biotransformation derived the triglycerides in castor oil by Yarrowia lipolytica , has been widely described literature studies concerning lipidic metabolism that leads to lactones production, interactions of cells with lipid substrate, toxicity produced metabolites, selection over‐producing mutants and environmental conditions. RESULTS In order improve technological aspects γ‐decalactone oxygen transfer rate ( OTR ), cell...
Abstract In recent years microorganisms have been engineered towards synthesizing interesting plant polyphenols such as flavonoids and stilbenes from glucose. Currently, the low endogenous supply of malonyl‐CoA, indispensable for polyphenol synthesis, impedes high product titers. Usually, limited malonyl‐CoA availability during production is avoided by supplementing fatty acid synthesis‐inhibiting antibiotics cerulenin, which are known to increase intracellular pool a side effect. Motivated...
Abstract γ‐Decalactone is an industrially interesting peach‐like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) raw material most used as acid source. The effect different CO concentrations on γ‐decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L −1 found to optimal concentration. Under these conditions, cells were able produce lipase but at low activity levels, which might...
Edible berries are considered to be among nature's treasure chests as they contain a large number of (poly)phenols with potentially health-promoting properties. However, complex (poly)phenol mixtures, it is challenging associate any interesting pharmacological activity single compound. Thus, identification pharmacologically phenols requires systematic analyses berry extracts. Here, raspberry (Rubusidaeus, var Prestige) extracts were systematically analyzed identify bioactive compounds...
Abstract γ‐Decalactone is an aromatic compound of industrial interest, resulting from the biotransformation ricinoleic acid, major constituent castor oil. In order to increase availability substrate cells for aroma production, oil previously hydrolyzed can be used. This hydrolysis may promoted by enzymatic action, more specifically lipases. this work, influence upon production lipase produced Yarrowia lipolytica , a microorganism able carry out biotransformation, was studied. first approach,...
Abstract Zymomonas mobilis is a well-recognized ethanologenic bacterium with outstanding characteristics which make it promising platform for the biotechnological production of relevant building blocks and fine chemicals compounds. In last years, research has been focused on physiological, genetic, metabolic engineering strategies aiming at expanding Z. ability to metabolize lignocellulosic substrates toward biofuel production. With expansion molecular computational modeling toolbox,...
At present five urea cycle diseases have been described, each representing a defect in the biosynthesis of one enzymes that metabolic pathway. Four these diseases-deficiencies carbamyl phosphate synthetase, ornithine transcar-bamylase, argininosuccinic acid and argininosuccinase-have similar clinical presentations (the most severe expression is symptomatic neonatal hyperammonemia, but they may also manifest as episodic changes mental state, which appear from infancy to adulthood), result...
E. coli was used to produce chondroitin both in silico and vivo , marking the first use of genome-scale metabolic models predict its production. Identified targets can guide future works towards more efficient hosts for
Abstract BACKGROUND 2‐Phenylethanol (2‐PE) is an aromatic alcohol with a delicate fragrance of rose petals that widely used in perfumes, cosmetics, foods, beverages, and pharmaceutical industries. The yeast Yarrowia can produce 2‐PE through L‐Phenylalanine (L‐Phe) biotransformation. However, it has not been properly explored this field, despite its interesting characteristics for process, such as the Crabtree negative trait. RESULTS This study potential different species ( Y. lipolytica W29,...
Abstract BACKGROUND The growing interest in polyphenols has led to the design of industrial‐scale processes able produce them by fermentation and recover a more sustainable way. goal this work is present two integrated approaches for recovery resveratrol, obtained through fermentation. production resveratrol using Corynebacterium glutamicum its continuous removal hydrophobic resin described. Batch compared with situ product removal, where Amberlite XAD‐7HP either directly added medium...
Epilactose is a functional sugar that can be produced from lactose using cellobiose 2-epimerase and it considered developing prebiotic. In sense, the development of strategies to produce purify epilactose key for its wider use in food industry. The aim this work was establish food-grade purification strategy suitable scaled-up an industrial level. Firstly, by enzymatic epimerization reaction catalyzed recombinant Caldicellulosiruptor saccharolyticus Saccharomyces cerevisiae. Then, remove...
Prevention of bacterial adhesion is a way to reduce and/or avoid biofilm formation, thus restraining its associated infections. The development repellent anti-adhesive surfaces, such as superhydrophobic can be strategy adhesion. In this study, polyethylene terephthalate (PET) film was modified by in situ growth silica nanoparticles (NPs) create rough surface. surface further with fluorinated carbon chains increase hydrophobicity. PET surfaces presented pronounced character, showing water...