A5008059944- RNA Research and Splicing
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Physiological and biochemical adaptations
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- Animal Genetics and Reproduction
- Genetics, Bioinformatics, and Biomedical Research
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Plant Reproductive Biology
- Viral Infectious Diseases and Gene Expression in Insects
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Protist diversity and phylogeny
- Silk-based biomaterials and applications
- Insect and Arachnid Ecology and Behavior
- Molecular Biology Techniques and Applications
- Science, Research, and Medicine
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
University of Cambridge
2015-2024
University of Oxford
2009-2012
RTX (United States)
2011
Princeton University
2006-2009
University Medical Center Utrecht
2009
Vehicular networking has significant potential to enable diverse applications associated with traffic safety, efficiency and infotainment.In this survey tutorial paper we introduce the basic characteristics of vehicular networks, provide an overview requirements, along challenges their proposed solutions.In addition, current past major ITS programs projects in USA, Japan Europe.Moreover, architectures protocol suites employed such Europe are discussed.
Ribonucleoprotein condensates can exhibit diverse physical states in vitro and vivo. Despite considerable progress, the relevance of condensate for vivo biological function remains limited. Here, we investigated properties processing bodies (P bodies) their impact on mRNA storage mature Drosophila oocytes. We show that conserved DEAD-box RNA helicase Me31B forms viscous P body condensates, which adopt an arrested state. demonstrate structurally distinct proteins protein-protein interactions,...
During Drosophila oogenesis, the targeted localization of gurken (grk) mRNA leads to establishment axis polarity egg. In early stages grk is found at posterior oocyte, whereas in later positioned dorsal anterior corner oocyte. order visualize real-time and anchorage endogenous living oocytes, we have utilized MS2-MCP system. We show that MCP-GFP-tagged localizes properly within wild-type oocytes behaves aberrantly mutant backgrounds. Fluorescence recovery after photobleaching (FRAP)...
Centrosomes have many important functions and comprise a 'mother' 'daughter' centriole surrounded by pericentriolar material (PCM). The mother recruits organises the PCM templates formation of daughter centriole. It has been reported that several Drosophila PCM-organising proteins are recruited to centrioles from cytosol as part large cytoplasmic 'S-CAP' complexes contain protein Sas-4. In previous paper (Conduit et al., 2014b) we showed one these proteins, Cnn, another key protein, Spd-2,...
In Drosophila oocytes, gurken/TGF-α mRNA is essential for establishing the future embryonic axes. gurken remains translationally silent during transport from its point of synthesis in nurse cells to final destination oocyte, where it associates with edge processing bodies. Here we show that, cells, kept by lack sufficient Orb/CPEB, translational activator. Processing bodies have a similar protein complement and ultrastructure those but they markedly less Orb do not associate mRNA. Ectopic...
Activation is an essential process that accompanies fertilisation in all animals and heralds major cellular changes, most notably, resumption of the cell cycle. While activation involves wave-like oscillations intracellular Ca2+ concentration mammals, ascidians polychaete worms a single peak fish frogs, insects, such as Drosophila, to date, it has not been shown what changes levels occur. Here, we utilise ratiometric imaging indicator dyes genetically encoded proteins identify characterise...
Abstract Many amniote vertebrate species including humans can form identical twins from a single embryo, but this only occurs rarely. It has been suggested that the primitive-streak-forming embryonic region emits signals inhibit streak formation elsewhere involved, how they are transmitted and act not elucidated. Here we show short tracks of calcium firing activity propagate through extraembryonic tissue via gap junctions prevent ectopic primitive in chick embryos. Cross-regulation an...
Localization of bicoid mRNA to the anterior Drosophila oocyte is essential for patterning anteroposterior body axis in early embryo. localizes a complex multistep process involving transacting factors, molecular motors and cytoskeletal components that remodel extensively during lifetime mRNA. Genetic requirements several localization including Swallow Staufen, are well established, but precise roles these factors their relationship transport particles remains unresolved. Here we use live...
Meiosis is a highly conserved feature of sexual reproduction that ensures germ cells have the correct number chromosomes prior to fertilization. A subset microtubules, known as spindle, are essential for accurate chromosome segregation during meiosis. Building evidence in mammalian systems has recently highlighted unexpected requirement actin cytoskeleton segregation; network spindle filaments appear regulate many aspects this process. Here we show Drosophila oocytes also population appears...
The transition from oocyte to embryo requires translation of maternally provided transcripts that in Drosophila is activated by Pan Gu kinase release a rapid succession 13 mitotic cycles. Mitotic entry promoted several protein kinases include Greatwall/Mastl, whose Endosulfine substrates antagonize Protein Phosphatase 2A (PP2A), facilitating Cyclin-dependent 1/Cyclin B activity. Here we show hyperactive greatwallScant can not only be suppressed mutants its Endos substrate but also subunits....
Abstract Egg activation is the essential process in which mature oocytes gain competency to proceed into embryonic development. Many events of egg are conserved, including an initial rise intracellular calcium. In some species, such as echinoderms and mammals, changes actin cytoskeleton occur around time fertilization activation. However, interplay between calcium during remains unclear. Here, we use imaging, genetics, pharmacological treatment, physical manipulation elucidate relationship...
Live cell imaging is an important technique applied to a number of Drosophila tissues used as models investigate topics such axis specification, differentiation and organogenesis 1. Correct preparation the experimental samples crucial, often neglected, step. The goal ensure physiological relevance establish optimal conditions. To maintain tissue viability, it critical avoid dehydration, hypoxia, overheating or medium deterioration 2. egg chamber well established system for examining...
Conducting a risk assessment (RA) for cloud computing platforms presents new challenges in the space of Information Security Management Systems (ISMS). ISO 27001 RA methods have been used Alcohol Monitoring (AMS) across portfolio products and services. Scaling these localized techniques to national international security standards shows that `one size fits all' approach does not exist industry today. The context conducting are examined representative guidelines.
Egg activation is a series of highly coordinated processes that prepare the mature oocyte for embryogenesis. Typically associated with fertilization, egg results in many downstream outcomes, including resumption meiotic cell cycle, translation maternal mRNAs and cross-linking vitelline membrane. While some aspects activation, such as initiation factors mammals environmental cues sea animals, have been well-documented, mechanics insects are less well-understood. For insects, can be triggered...
Localized mRNA translation is a widespread mechanism for targeting protein synthesis, important cell fate, motility and pathogenesis. In Drosophila, the spatiotemporal control of gurken/TGF-α required establishing embryonic body axes. A number recent studies have highlighted key aspects gurken translational at dorsoanterior corner mid-stage oocyte. Orb/CPEB Wispy/GLD-2 are polyadenylation mRNA, but unlocalized in oocyte not fully polyadenylated. 1 At corner, Orb been shown to be enriched...
ABSTRACT Biomolecular condensates that form via liquid-liquid phase separation can exhibit diverse physical states. Despite considerable progress, the relevance of condensate states for in vivo biological function remains limited. Here, we investigated properties processing bodies (P bodies) and their impact on mRNA storage mature Drosophila oocytes. We show conserved DEAD-box RNA helicase Me31B forms P body which adopt a less dynamic, arrested state. demonstrate structurally distinct...