A5030734756- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Nuclear Structure and Function
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- 14-3-3 protein interactions
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Cellular transport and secretion
- Reproductive Biology and Fertility
University of Oxford
2014-2022
Centrosomes comprise a pair of centrioles surrounded by pericentriolar material (PCM). The PCM expands dramatically as cells enter mitosis, but it is unclear how this occurs. In study, we show that the centriole protein Asl initiates recruitment DSpd-2 and Cnn to mother centrioles; both proteins then assemble into co-dependent scaffold-like structures spread outwards from recruit most, if not all, other components. absence either or Cnn, mitotic assembly diminished; in proteins, appears be...
Centrioles form centrosomes and cilia, defects in any of these three organelles are associated with human disease [1]. duplicate once per cell cycle, when a mother centriole assembles an adjacent daughter during S phase. Daughter centrioles cannot support the assembly another until they mature into mothers next cycle [2-5]. The molecular nature this daughter-to-mother transition remains mysterious. Pioneering studies C. elegans identified set core proteins essential for duplication [6-12],...
Centrioles are highly structured organelles whose size is remarkably consistent within any given cell type. New centrioles born when Polo-like kinase 4 (Plk4) recruits Ana2/STIL and Sas-6 to the side of an existing “mother” centriole. These two proteins then assemble into a cartwheel, which grows outwards form structural core new daughter. Here, we show that in early Drosophila melanogaster embryos, daughter grow at linear rate during S-phase abruptly stop growing they reach their correct...
Centrosomes and cilia are organized by a centriole pair comprising an older mother younger daughter. Centriole numbers tightly regulated, daughter centrioles (which assemble in S phase) cannot themselves duplicate or organize centrosomes until they have passed through mitosis. It is unclear how this mitotic "centriole conversion" but it requires Plk1/Polo kinase. Here we show that flies, Cdk1 phosphorylates the conserved protein Sas-4 during This creates Polo-docking site helps recruit Polo...
Centrosomes are formed when mother centrioles recruit pericentriolar material (PCM) around themselves. The PCM expands dramatically as cells prepare to enter mitosis (a process termed centrosome maturation), but it is unclear how this expansion achieved. In flies, Spd-2 and Cnn thought form a scaffold the centriole that recruits other components of mitotic PCM, Polo-dependent phosphorylation at crucial for assembly. Here, we show that, like Cnn, specifically phosphorylated centrosomes. This...
The accurate timing and execution of organelle biogenesis is crucial for cell physiology. Centriole regulated by Polo-like kinase 4 (Plk4) initiates in S-phase when a daughter centriole grows from the side pre-existing mother. Here, we show that Plk4 oscillation at base growing times to ensure centrioles grow right time size. normally entrained cell-cycle oscillator but can run autonomously it-potentially explaining why duplicate independently progression. Mathematical modeling indicates be...
Centrosomes have many important functions and comprise a 'mother' 'daughter' centriole surrounded by pericentriolar material (PCM). The mother recruits organises the PCM templates formation of daughter centriole. It has been reported that several Drosophila PCM-organising proteins are recruited to centrioles from cytosol as part large cytoplasmic 'S-CAP' complexes contain protein Sas-4. In previous paper (Conduit et al., 2014b) we showed one these proteins, Cnn, another key protein, Spd-2,...
Centrioles organise centrosomes and cilia, these organelles have an important role in many cell processes. In flies, the centriole protein Ana1 is required for assembly of functional cilia. It has recently been shown that Cep135 (also known as Bld10) initially recruits to newly formed centrioles, then Asl (known Cep152 mammals) promote conversion centrioles into centrosomes. Here, we show ana1 mutants lack detectable vivo, irreversibly incorporated during their appears play a more...
Acentriolar microtubule organizing centers (aMTOCs) are formed during meiosis and mitosis in several cell types, but their function assembly mechanism is unclear. Importantly, aMTOCs can be overactive cancer cells, enhancing multipolar spindle formation, merotelic kinetochore attachment aneuploidy. Here we show that form acentriolar Drosophila somatic cells vivo via an pathway depends on Asl, Cnn and, to a lesser extent, Spd-2--the same proteins appear drive mitotic centrosome flies. This...
Centrioles duplicate once per cell cycle, but it is unclear how daughter centrioles assemble at the right time and place grow to size. Here, we show that in Drosophila embryos cytoplasmic concentrations of key centriole assembly proteins Asl, Plk4, Ana2, Sas-6, Sas-4 are low, remain constant throughout process—indicating none them limiting for assembly. The diffusion rate Ana2/STIL, however, increased significantly toward end S-phase as Cdk/Cyclin activity embryo increased. A mutant form...
Abstract Centrosomes are formed when mother centrioles recruit pericentriolar material (PCM) around themselves. The PCM expands dramatically as cells prepare to enter mitosis (a process termed centrosome maturation), but it is unclear how this expansion achieved. In flies, Spd-2 and Cnn form an extensive scaffold the centriole that recruits other components of mitotic PCM, Polo-dependent phosphorylation at crucial for assembly. Here we show that, like Cnn, specifically phosphorylated...
Abstract Centrioles duplicate once per cell cycle but it is unclear how daughter centrioles assemble at the right time and place grow to size. Here we show that in early Drosophila embryos cytoplasmic concentrations of key centriole assembly proteins Asl, Plk4, Ana2, Sas-6 Sas-4 are low, remain constant throughout process— indicating none them limiting for assembly. The diffusion rate Ana2/STIL, however, increased significantly towards end S-phase as Cdk/Cyclin activity embryo increased. A...