A5043431299- Cervical Cancer and HPV Research
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Nonmelanoma Skin Cancer Studies
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- Cancer and Skin Lesions
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- 14-3-3 protein interactions
- RNA modifications and cancer
- Tissue Engineering and Regenerative Medicine
- RNA and protein synthesis mechanisms
- Nuclear Structure and Function
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
University of Oxford
2018-2025
Institute of Molecular Biotechnology
2022
Austrian Academy of Sciences
2022
Vienna Biocenter
2022
Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. The PCM comprises hundreds proteins, and there is much debate about its physical nature. Here, we show that Drosophila Spd-2 (human CEP192) fluxes out from centrioles, recruiting Polo Aurora A kinases to catalyze the assembly two distinct mitotic-PCM scaffolds: a Polo-dependent Cnn scaffold, an A-dependent TACC which exhibit solid- liquid-like behaviors, respectively. Both...
Centrioles are highly structured organelles whose size is remarkably consistent within any given cell type. New centrioles born when Polo-like kinase 4 (Plk4) recruits Ana2/STIL and Sas-6 to the side of an existing “mother” centriole. These two proteins then assemble into a cartwheel, which grows outwards form structural core new daughter. Here, we show that in early Drosophila melanogaster embryos, daughter grow at linear rate during S-phase abruptly stop growing they reach their correct...
Abstract The skin is exposed to viral pathogens, but whether they contribute the oncogenesis of cancers has not been systematically explored. Here we investigated 19 tumor types by analyzing off-target reads from commonly available next-generation sequencing data for pathogens. We identified human papillomavirus 42 (HPV42) in 96% (n = 45/47) digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on fingers and toes. show that HPV42, so far considered a nononcogenic,...
Centrosomes are formed when mother centrioles recruit pericentriolar material (PCM) around themselves. The PCM expands dramatically as cells prepare to enter mitosis (a process termed centrosome maturation), but it is unclear how this expansion achieved. In flies, Spd-2 and Cnn thought form a scaffold the centriole that recruits other components of mitotic PCM, Polo-dependent phosphorylation at crucial for assembly. Here, we show that, like Cnn, specifically phosphorylated centrosomes. This...
The accurate timing and execution of organelle biogenesis is crucial for cell physiology. Centriole regulated by Polo-like kinase 4 (Plk4) initiates in S-phase when a daughter centriole grows from the side pre-existing mother. Here, we show that Plk4 oscillation at base growing times to ensure centrioles grow right time size. normally entrained cell-cycle oscillator but can run autonomously it-potentially explaining why duplicate independently progression. Mathematical modeling indicates be...
Accurate repair of DNA double-strand breaks (DSBs) is essential for genome stability, and defective underlies diseases such as cancer. Homologous recombination uses an intact homologous sequence to faithfully restore damaged DNA, yet how broken ends find sites in a containing billions non-homologous bases remains unclear. Here, we introduce sister-pore-C, high-resolution method mapping intra- trans-molecular interactions replicated chromosomes. We show that DSBs reshape chromosome...
A deeply conserved miR-1–dependent regulon of mitochondria and lysosome function is essential for muscle physiology.
Centrioles are composed of a central cartwheel tethered to nine-fold symmetric microtubule (MT) blades. The centriole and MTs thought grow from opposite ends these organelles, so it is unclear how they coordinate their assembly. We previously showed that in Drosophila embryos an oscillation Polo-like kinase 4 (Plk4) helps initiate time the growth at proximal end. Here, same model, we show CP110 Cep97 form complex close distal-end whose levels rise fall as new grow, manner appears be...
Centrioles duplicate once per cell cycle, but it is unclear how daughter centrioles assemble at the right time and place grow to size. Here, we show that in Drosophila embryos cytoplasmic concentrations of key centriole assembly proteins Asl, Plk4, Ana2, Sas-6, Sas-4 are low, remain constant throughout process—indicating none them limiting for assembly. The diffusion rate Ana2/STIL, however, increased significantly toward end S-phase as Cdk/Cyclin activity embryo increased. A mutant form...
Abstract Mitotic centrosomes form when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. The PCM comprises hundreds proteins, yet it can assemble and disassemble within minutes, leading to much debate about its physical nature. Here we show that Drosophila Spd-2 fluxes out from centrioles, recruiting Polo Aurora A catalyse the assembly a solid-like Cnn-scaffold more liquid-like TACC-scaffold, respectively. Both scaffolds proteins independently, but both are...
Abstract The accurate timing of organelle biogenesis and the precise regulation size are crucial for cell physiology. Centriole initiates exclusively in S-phase, when a daughter centriole emerges from side pre-existing mother grows until it reaches its mother’s size. This process is regulated by Polo-like kinase 4 (Plk4), which recruited to centrioles oscillatory waves flies human cells 1,2 . nature function Plk4 oscillations is, however, unknown. Here we discover that forms an adaptive...
Muscles are not only essential for force generation but also key regulators of systemic energy homeostasis 1 . Both these roles rely heavily on mitochondria and lysosome function as providers building blocks, metabolic sensors 2-4 Perturbations in organelles or their crosstalk lead to a wide range pathologies 5 Here, we uncover deeply conserved regulon under control the muscle-specific microRNA miR-1. Animals lacking miR-1 display diverse muscle cell defects that have been attributed...
Summary Centrioles are composed of a central cartwheel tethered to nine-fold symmetric microtubule (MT) blades. The centriole and MTs thought grow from opposite ends these organelles, so it is unclear how they coordinate their assembly. We previously showed that an oscillation Polo-like kinase 4 (Plk4) helps initiate time the growth at proximal end. Here, we show CP110 Cep97 form complex close distal-end whose levels rise fall as new grow, entrained by core Cdk/Cyclin oscillator drives...
<div>Abstract<p>The skin is exposed to viral pathogens, but whether they contribute the oncogenesis of cancers has not been systematically explored. Here we investigated 19 tumor types by analyzing off-target reads from commonly available next-generation sequencing data for pathogens. We identified human papillomavirus 42 (HPV42) in 96% (<i>n</i> = 45/47) digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on fingers and toes. show that HPV42, so...
<p>Supplementary data depicted in Supplementary Figures S1-S9</p>
<div>Abstract<p>The skin is exposed to viral pathogens, but whether they contribute the oncogenesis of cancers has not been systematically explored. Here we investigated 19 tumor types by analyzing off-target reads from commonly available next-generation sequencing data for pathogens. We identified human papillomavirus 42 (HPV42) in 96% (<i>n</i> = 45/47) digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on fingers and toes. show that HPV42, so...
<p>Supplementary data depicted in Supplementary Figures S1-S9</p>