A5039207382- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Gene Regulatory Network Analysis
- Developmental Biology and Gene Regulation
- Evolution and Genetic Dynamics
- Adipose Tissue and Metabolism
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Ship Hydrodynamics and Maneuverability
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Fluid Dynamics Simulations and Interactions
- Peptidase Inhibition and Analysis
- Congenital heart defects research
- thermodynamics and calorimetric analyses
- Plant Molecular Biology Research
- Cardiac Fibrosis and Remodeling
- Light effects on plants
- Neurogenesis and neuroplasticity mechanisms
Research Institute of Molecular Pathology
2017-2025
Austrian Academy of Sciences
2023-2025
Institute of Molecular Biotechnology
2023-2025
Vienna Biocenter
2017-2024
Chongqing University of Technology
2020
Beijing Institute of Technology
2020
École Polytechnique Fédérale de Lausanne
2013-2018
SIB Swiss Institute of Bioinformatics
2013-2015
Soochow University
2014-2015
Centre National de la Recherche Scientifique
2014
Significance Circadian clocks orchestrate daily rhythms in behavior and physiology using temporal regulation of gene expression to control core clock genes rhythmic output programs. Although transcription was shown drive extensive diurnal mRNA rhythms, less is known about the proteins. Here, we provide a proteome-wide study protein accumulation mouse liver, showing that proteins preferentially accumulate morning during night. About one-half these could not be explained by mRNAs, suggesting...
Diurnal oscillations of gene expression are a hallmark rhythmic physiology across most living organisms. Such controlled by the interplay between circadian clock and feeding rhythms. Although mRNA accumulation has been extensively studied, comparatively less is known about their transcription translation. Here, we quantified simultaneously temporal transcription, accumulation, translation mouse liver mRNAs under physiological light-dark conditions ad libitum or night-restricted in WT brain...
Diurnal oscillations of gene expression controlled by the circadian clock and its connected feeding rhythm enable organisms to coordinate their physiologies with daily environmental cycles. While available techniques yielded crucial insights into regulation at transcriptional level, much less is known about temporally functions within nucleus protein level. Here, we quantified temporal nuclear accumulation proteins phosphoproteins from mouse liver SILAC proteomics. We identified around 5,000...
The salamander limb correctly regenerates missing segments because connective tissue cells have segment-specific identities, termed "positional information". How positional information is molecularly encoded at the chromatin level has been unknown. Here, we performed genome-wide profiling in mature and regenerating axolotl cells. We find levels of histone H3K27me3 as major mark, especially homeoprotein gene loci but not their upstream regulators, constituting an intrinsic segment code....
Lysine acetylation is involved in various biological processes and considered a key reversible post-translational modification the regulation of gene expression, enzyme activity, subcellular localization. This therefore highly relevant context circadian biology, but its characterization on proteome-wide scale clock dependence are still poorly described. Here, we provide comprehensive rhythmic acetylome map mouse liver. Rhythmic acetylated proteins showed localization-specific phases that...
Significance Rhythms in gene expression propelled by the circadian clock and environmental signals are ubiquitous across cells tissues. In particular, mouse tissues, thousands of transcripts show oscillations with a period 24 hours. Keys question how such rhythms propagate eventually exert functions, but also these generated. Here, we developed mathematical model based on total RNA-seq to classify genes according respective contributions transcriptional posttranscriptional regulation toward...
Abstract Polycomb group (PcG) proteins play critical roles in the epigenetic inheritance of cell fate. The Repressive Complexes PRC1 and PRC2 catalyse distinct chromatin modifications to enforce gene silencing, but how transcriptional repression is propagated through mitotic divisions remains a key unresolved question. Using reversible tethering PcG ectopic sites mouse embryonic stem cells, here we show that can trigger Polycomb-dependent modifications. We find canonical (cPRC1), not variant...
The transcriptional repressors Polycomb repressive complex 1 (PRC1) and PRC2 are required to maintain cell fate during embryonic development. PRC1 catalyze distinct histone modifications, establishing chromatin at shared targets. How PRC1, which consists of canonical (cPRC1) variant (vPRC1) complexes, cooperate silence genes support mouse stem (mESC) self-renewal is unclear. Using combinatorial genetic perturbations, we show that independent pathways cPRC1 vPRC1 responsible for maintenance...
Combinatorial action of transcription factors (TFs) with partially overlapping expression is a widespread strategy to generate novel gene-expression patterns and, thus, cellular diversity. Known mechanisms underlying combinatorial activity require co-expression TFs within the same cell. Here, we describe mechanism by which two that are never co-expressed new, intersectional pattern in C. elegans embryos: lineage-specific priming gene transiently expressed TF generates unique intersection...
Abstract Primary body axis development is a highly conserved process that proceeds through somitogenesis and further subdivision into dermatome, myotome, sclerotome. Defects in somitic-clock genes such as Hes7 lead to vertebral segmentation defects mice fish. Here we show the axolotl, also necessary for proper embryonic but is, surprisingly, dispensable during axolotl tail regeneration. Using genetic barcoding fate mapping found regeneration, somitic derivatives (muscle, cartilage, tendon,...
During neural tube (NT) development, the notochord induces an organizer, floorplate, which secretes Sonic Hedgehog (SHH) to pattern progenitors. Conversely, NT organoids (NTOs) from embryonic stem cells (ESCs) spontaneously form floorplates without notochord, demonstrating that can self-organize inducers. Here, we investigated floorplate self-organization in clonal mouse NTOs. Expression of marker FOXA2 was initially spatially scattered before resolving into multiple clusters, underwent...
A deeply conserved miR-1–dependent regulon of mitochondria and lysosome function is essential for muscle physiology.
MicroRNAs (miRNAs) are a class of post-transcriptional repressors with diverse roles in animal development and physiology [1]. The Microprocessor complex, composed Drosha Pasha/DGCR8, is necessary for the biogenesis all canonical miRNAs essential early stages embryogenesis [2-8]. However, cause this requirement largely unknown. Animals often express hundreds miRNAs, it remains unclear whether required to produce one or few many individually non-essential miRNAs. Additionally, both...
A preparative high-speed counter-current chromatography method for isolation and purification of bufadienolides from ChanSu was developed by using a stepwise elution with two-phase solvent system composed n-hexane/ethyl acetate/methanol/water at the ratios 4:6:2:4 v/v, 4:6:2.5:4 v/v 4:6:3.2:4 v/v. total 3.8 mg gamabufotalin (1), 7.2 arenobufagin (2), 3.4 telocinobufagin (3), 5.3 bufotalin (4), 8.5 cinobufotalin (5) 8 bufalin (6) were obtained in one-step separation 80 crude extract purity...
Fundamental biological processes such as transcription and translation, where a genetic sequence is sequentially read by macromolecule, have been well described classical model of non-equilibrium statistical physics, the totally asymmetric exclusion principle (TASEP). This describes particles hopping between sites one-dimensional lattice, with particle current determining or translation rate. An open problem how to analyze TASEP can pause randomly, has observed during transcription. In this...
The ship wave is of great interest for drag and coastal erosion. This paper proposes a mechanism transformation to explore the effects speed size on waveform. Firstly, based theory potential flow, boundary integral equations Kelvin waves are obtained by deploying different sources or Rankine sources. Then, these numerically discretized set nonlinear equations. Finally, Jacobian−free Newton–Krylov method with preconditioner adopted solve Though imitating plenty patterns, proposed conveniently...
Abstract Fibrosis is a broad pathology of excessive scarring with substantial medical implications. The fibrotic scar produced by myofibroblasts that interact macrophages. complex process involving thousands factors, therefore, to better understand fibrosis and develop new therapeutic approaches, it necessary simplify clarify the underlying concepts. Recently, we described mathematical model for macrophage-myofibroblast cell circuit, predicting two types - hot abundant macrophages...
Adaptation of the functional proteome is essential to counter pathogens during infection, yet precisely timed degradation these response proteins after pathogen clearance likewise key preventing autoimmunity. Interferon regulatory factor 1 (IRF1) plays an role as a transcription in driving expression immune genes infection. The striking difference output with other IRFs that IRF1 also drives various cell cycle inhibiting factors, making it important tumor suppressor. Thus, critical regulate...