A5039977263- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Silk-based biomaterials and applications
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Wound Healing and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Planarian Biology and Electrostimulation
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Skin Protection and Aging
- Morphological variations and asymmetry
- Retinal Development and Disorders
- Genetics and Physical Performance
- Marine Ecology and Invasive Species
Mount Desert Island Biological Laboratory
2020-2025
Medizinische Hochschule Hannover
2020-2025
Research Institute of Molecular Pathology
2018-2024
TU Dresden
2012-2024
Vienna Biocenter
2018-2020
Deutsche Forschungsgemeinschaft
2014-2018
National Centre for Cell Science
2009-2013
Berlin-Brandenburger Centrum für Regenerative Therapien
2013
National Chemical Laboratory
2011
Maharaja Sayajirao University of Baroda
2010
How the axolotl makes a new limb Unlike most vertebrate limbs, regenerates skeleton after amputation. Dermal and interstitial fibroblasts have been thought to provide sources for skeletal regeneration, but it has unclear whether preexisting stem cells or dedifferentiation of formed blastema. Gerber et al. developed transgenic reporter animals compare periskeletal cell fibroblast contributions regeneration. Callus-forming extended existing bone, built segments. Single-cell transcriptomics...
Turbidity and opaqueness are inherent properties of tissues which limit the capacity to acquire microscopic images through large tissues. Creating a uniform refractive index, known as tissue clearing, overcomes most these issues. These methods have enabled researchers image complex 3D structures with unprecedented depth resolution. However, clearing has been adopted limited extent due combination cost, time, complexity existing potential negative impact on fluorescence signal. Here we...
Limb regeneration, while observed lifelong in salamanders, is restricted post-metamorphic Xenopus laevis frogs. Whether this loss due to systemic factors or an intrinsic incapability of cells form competent stem has been unclear. Here, we use genetic fate mapping establish that connective tissue (CT) the frog blastema, as case axolotls. Using heterochronic transplantation into limb bud and single-cell transcriptomic profiling, show axolotl CT dedifferentiate integrate lineages, including...
A tailored protocol combines pigment removal with clearing, facilitating labeling and whole-body imaging across phylogeny.
The salamander limb correctly regenerates missing segments because connective tissue cells have segment-specific identities, termed "positional information". How positional information is molecularly encoded at the chromatin level has been unknown. Here, we performed genome-wide profiling in mature and regenerating axolotl cells. We find levels of histone H3K27me3 as major mark, especially homeoprotein gene loci but not their upstream regulators, constituting an intrinsic segment code....
Abstract Primary body axis development is a highly conserved process that proceeds through somitogenesis and further subdivision into dermatome, myotome, sclerotome. Defects in somitic-clock genes such as Hes7 lead to vertebral segmentation defects mice fish. Here we show the axolotl, also necessary for proper embryonic but is, surprisingly, dispensable during axolotl tail regeneration. Using genetic barcoding fate mapping found regeneration, somitic derivatives (muscle, cartilage, tendon,...
ABSTRACT The degenerative loss of muscle associated with aging leading to muscular atrophy is called sarcopenia. Currently, practicing regular physical exercise the only efficient way delay sarcopenia onset. Identification therapeutic targets alleviate symptoms requires in vivo model organisms accelerated degeneration and atrophy. zebrafish undergoes aging, hallmarks including mitochondrial dysfunction, telomere shortening, accumulation senescent cells. However, age slowly, no specific...
Axolotls ( Ambystoma mexicanum ) are known for their remarkable limb-regeneration abilities, which involve the formation of blastema, a specialized structure consisting progenitor cells contributed by all major tissues limb. Lateral plate mesoderm (LPM)-derived connective tissue (CT) dedifferentiate and play critical role in blastema subsequent limb regeneration. However, complexity blastema's cellular composition extent CT participation necessity have not been rigorously explored. To...
The preparation of highly stable water dispersions silver nanoparticles using the naturally available gellan gum as a reducing and capping agent is reported. Further, exploiting gel formation characteristic nanoparticle incorporated gels have also been prepared. optical properties, morphology, zeta potential long-term stability synthesized were investigated. superior gum-silver against pH variation electrolyte addition revealed. Finally, we studied cytotoxicity AgNP in mouse embryonic...
The heterogeneous properties of dermal cell populations have been posited to contribute toward fibrotic, imperfect wound healing in mammals. Here we characterize an adult population fibroblasts that maintain active Prrx1 enhancer which originally marked mesenchymal limb progenitors. In contrast their abundance development, postnatal enhancer-positive cells (Prrx1enh+) make up a small subset (∼0.2%) and reside mainly within perivascular hair follicle niches. Lineage tracing Prrx1enh+ shows...
A rapid method for temporally and spatially controlled CRISPR-mediated gene knockout in vertebrates will be an important tool to screen genes involved complex biological phenomena like regeneration. Here we show that vivo injection of CAS9 protein-guide RNA (gRNA) complexes into the spinal cord lumen axolotl subsequent electroporation leads comprehensive Sox2 expression SOX2+ neural stem cells with corresponding functional phenotypes from knockout. This is particularly surprising considering...
Asymmetric localization of adenomatous polyposis coli (APC) to the ends a subset microtubules located in leading edges is essential for establishment front-rear polarity during cell migration. APC known associate with three ways: through interaction plus-end tracking protein EB1, direct binding C-terminal basic region, and motor kinesin-2. Here we report that middle region has previously unidentified microtubule plus-end-targeting function, suggesting an additional microtubule-binding mode...
Summary Cells often respond to diverse environmental stresses by inducing stress granules (SGs) as an adaptive mechanism. SGs are generally assembled a result of aggregation mRNAs stalled in translational pre-initiation complex, mediated set RNA-binding proteins such G3BP and TIA-1. may serve triage centres for storage, translation re-initiation or degradation specific mRNAs. However, the mechanism involved modulation their assembly/disassembly is unclear. Here we report that Wnt signalling...
The axolotl Ambystoma mexicanum is one of the most used model organisms in developmental and regenerative studies because it commonly said that can reconstitute a normal fully functional forelimb/hindlimb after amputation. However, there not publication has described detail regeneration hindlimb muscles. Here we describe illustrate, for first time, thigh, leg foot muscles transgenic axolotls express green fluorescent protein muscle fibers compare our results with data obtained by us other...
Summary Par polarity complex, consisting of Par3, Par6, and aPKC, plays a conserved role in the establishment maintenance polarization diverse cellular contexts. Recent reports suggest that Dishevelled (Dvl), cytoplasmic mediator Wnt signalling, interacts with atypical protein kinase C regulates its activity during neuronal differentiation directed cell migration. Here we show Nup358 (also called RanBP2), nucleoporin previously implicated migration, aPKC through N-terminal region (BPN)...
Abstract The laboratory axolotl ( Ambystoma mexicanum) is widely used in biological research. Recent advancements genetic and molecular toolkits are greatly accelerating the work using axolotl, especially area of tissue regeneration. At this juncture, there a critical need to establish gene transgenic nomenclature ensure uniformity Here, we propose guidelines for when working with axolotl.
Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need user-friendly system that can address applications with varied requirements in terms resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), compatibility tissue-clearing protocols holders various refractive indices. We present ‘hybrid’ combines novel non-orthogonal dual-objective conventional (orthogonal)...