A5062303415- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Force Microscopy Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Nanofabrication and Lithography Techniques
- Nicotinic Acetylcholine Receptors Study
- Cellular Mechanics and Interactions
- Photoreceptor and optogenetics research
- Single-cell and spatial transcriptomics
- Photoacoustic and Ultrasonic Imaging
- Near-Field Optical Microscopy
- Neuroinflammation and Neurodegeneration Mechanisms
- Optical Imaging and Spectroscopy Techniques
- Advanced Biosensing Techniques and Applications
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Microtubule and mitosis dynamics
- Visual perception and processing mechanisms
Allen Institute
2018-2024
Allen Institute for Brain Science
2018-2024
Allen Institute for Neural Dynamics
2023
University of Washington
2023
Recent advances in tissue processing, labeling, and fluorescence microscopy are providing unprecedented views of the structure cells tissues at sub-diffraction resolutions near single molecule sensitivity, driving discoveries diverse fields biology, including neuroscience. Biological is organized over scales nanometers to centimeters. Harnessing molecular imaging across three-dimensional samples on this scale requires new types microscopes with larger view working distance, as well higher...
Abstract Recent advances in tissue processing, labeling, and fluorescence microscopy are providing unprecedented views of the structure cells tissues at sub-diffraction resolutions near single molecule sensitivity, driving discoveries diverse fields biology, including neuroscience. Biological is organized over scales nanometers to centimeters. Harnessing molecular imaging across intact, three-dimensional samples on this scale requires new types microscopes with larger view working distance,...
Recent advances in tissue processing, labeling, and fluorescence microscopy are providing unprecedented views of the structure cells tissues at sub-diffraction resolutions near single molecule sensitivity, driving discoveries diverse fields biology, including neuroscience. Biological is organized over scales nanometers to centimeters. Harnessing molecular imaging across intact, three-dimensional samples on this scale requires new types microscopes with larger view working distance, as well...
Neuromodulators such as acetylcholine, noradrenaline (norepinephrine), and serotonin are released into the cortex by axons ascending from subcortical nuclei. These neuromodulators have been hypothesized to influence cortical function during behavioral periods arousal, locomotion, exploration, attention. To determine when these neuromodulatory projections were active, we expressed genetically-encoded calcium sensor GCaMP6 in which project mouse primary visual performed two-photon microscopy...
Abstract Progress in histological methods and microscope technology has enabled dense staining imaging of axons over large brain volumes, but tracing such volumes requires new computational tools for 3D reconstruction data acquired from serial sections. We have developed a pipeline automated volume assembly densely stained imaged sections, which leverages machine learning-based segmentation to enable stitching alignment with the axon traces themselves. validated this segmentation-driven...
Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need user-friendly system that can address applications with varied requirements in terms resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), compatibility tissue-clearing protocols holders various refractive indices. We present ‘hybrid’ combines novel non-orthogonal dual-objective conventional (orthogonal)...
Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need user-friendly system that can address applications with varied requirements in terms resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), compatibility tissue-clearing protocols holders various refractive indices. We present ‘hybrid’ combines novel non-orthogonal dual-objective conventional (orthogonal)...
Motion/direction-sensitive and location-sensitive neurons are the two major functional types in mouse visual thalamus that project to primary cortex (V1). It is under debate whether motion/direction-sensitive inputs preferentially target superficial layers V1, as opposed inputs, which middle layers. Here, by using calcium imaging measure activity of axons we find evidence against these cell-type-specific laminar biases at population level. Furthermore, an approach reconstruct axon arbors...
Recent advances in tissue processing, labeling, and fluorescence microscopy are providing unprecedented views of the structure cells tissues at sub-diffraction resolutions near single molecule sensitivity, driving discoveries diverse fields biology, including neuroscience. Biological is organized over scales nanometers to centimeters. Harnessing molecular imaging across intact, three-dimensional samples on this scale requires new types microscopes with larger view working distance, as well...
Expansion microscopy and light sheet imaging enable fine-scale resolution of intracellular features that comprise neural circuits. Most current techniques visualize sparsely distributed across whole brains or densely within individual brain regions. Here, we dense distributions immunolabeled proteins early visual cortical areas in adult macaque monkeys. This process may be combined with multiphoton magnetic resonance to produce multimodal atlases large, gyrencephalic brains.
3-photon excitation permits fluorescence microscopy through the intact skull. We demonstrate imaging of neuronal structure and calcium activity in a chronic transcranial window preparation up to 300 μm into brain below
Axonal connectomics entails tracing of dense axons over large brain volumes. Here, we describe a light-sheet imaging approach using scanned Bessel beam illumination and confocal slit detection the results simulation-guided optimization. We evaluate performance our for axon resolution segmentation.
The motion/direction-sensitive and location-sensitive neurons are two major functional types in mouse visual thalamus that project to the primary cortex (V1). It has been proposed mainly target superficial layers V1, contrast which middle layers. Here, by imaging calcium activities of axons we find no evidence for these cell-type specific laminar biases at population level. Furthermore, using a novel approach reconstruct single-axon structures with identified vivo response types, show that,...
Co-registration of neuronal structures between in vivo and ex imaging is necessary to study structure-function correspondence the mammalian brain. Here we describe a protocol based on tangential sectioning mouse This aligns two-photon calcium volumes with confocal registers same cortical both volumes. approach allows detailed analysis corresponding function structure these entities. For complete details use execution this protocol, please refer Zhuang et al. (2021).
We are applying light-sheet fluorescence microscopy with scanned Bessel beam illumination and confocal slit detection, combined tissue expansion microscopy, to image dense axons over large brain volumes for axonal connectomics.
Abstract The motion/direction-sensitive and location-sensitive neurons are two major functional types in mouse visual thalamus that project to the primary cortex (V1). It has been proposed mainly target superficial layers V1, contrast which middle layers. Here, by imaging calcium activities of axons we find no evidence for these cell-type specific laminar biases at population level. Furthermore, using a novel approach reconstruct single-axon structures with identified vivo response types,...