A5037011219- Reproductive System and Pregnancy
- Cell Image Analysis Techniques
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Radiomics and Machine Learning in Medical Imaging
- Advanced Fluorescence Microscopy Techniques
- Celiac Disease Research and Management
- Medical Imaging Techniques and Applications
- Reproductive Physiology in Livestock
- T-cell and B-cell Immunology
- Endometriosis Research and Treatment
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Mesenchymal stem cell research
- Optical Imaging and Spectroscopy Techniques
- Molecular Biology Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Pneumonia and Respiratory Infections
- Neuroinflammation and Neurodegeneration Mechanisms
- Respiratory viral infections research
- Photoacoustic and Ultrasonic Imaging
- Digestive system and related health
- Educational Games and Gamification
- IL-33, ST2, and ILC Pathways
- Microscopic Colitis
The University of Adelaide
1977-2024
University of Washington
2020-2022
Women's and Children's Hospital
1977
Abstract Interleukin 17-producing γδ T (γδT17) cells have unconventional trafficking characteristics, residing in mucocutaneous tissues but also homing into inflamed via circulation. Despite being fundamental to γδT17-driven early protective immunity and exacerbation of autoimmunity cancer, migratory cues controlling γδT17 cell positioning barrier recruitment inflammatory sites are still unclear. Here we show that constitutively express chemokine receptors CCR6 CCR2. While recruits resting...
Regulatory T (Treg) cell defects are implicated in disorders of embryo implantation and placental development, but the origins Treg dysfunction unknown. Here, we comprehensively analyzed phenotypes transcriptional profile peripheral blood cells individuals with early pregnancy failure (EPF). Compared to fertile subjects, EPF subjects had 32% fewer total 54% CD45RA+CCR7+ naive among CD4+ cells, an altered phenotype reduced transcription factor FOXP3 suppressive marker CTLA4 expression, lower...
Abstract Regulatory T (Treg) cells are a specialized CD4+ cell subpopulation that essential for immune homeostasis, tolerance, and protection against autoimmunity. There is evidence sex-steroid hormones estrogen progesterone modulate Treg abundance phenotype in women. Since natural oscillations these modified by hormonal contraceptives, we examined whether oral contraception (OC) use impacts related populations. were analyzed multiparameter flow cytometry peripheral blood collected across...
Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need user-friendly system that can address applications with varied requirements in terms resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), compatibility tissue-clearing protocols holders various refractive indices. We present ‘hybrid’ combines novel non-orthogonal dual-objective conventional (orthogonal)...
Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need user-friendly system that can address applications with varied requirements in terms resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), compatibility tissue-clearing protocols holders various refractive indices. We present ‘hybrid’ combines novel non-orthogonal dual-objective conventional (orthogonal)...
Abstract Objectives Intravenous infusion of Intralipid is an adjunct therapy in assisted reproduction treatment (ART) when immune‐associated infertility suspected. Here, we evaluated the effect on regulatory T cells (Treg cells), effector and plasma cytokines peripheral blood women undertaking IVF. Methods This prospective, observational pilot study assessed 14 exhibiting recurrent implantation failure, a clinical sign infertility. Peripheral was collected immediately prior to 7 days after...
Abstract Background: The composition of the tumor microenvironment (TME) is a major determinant response to therapy in many solid tumors. To-date, characterization TME has been based on limited analysis thin tissue sections. Here we demonstrate utility an end-to-end 3D spatial biology workflow, I/O Pro™, whole imaging, identify and quantify cells, lymphocytes, collagen features human FFPE samples. Methods: Five colorectal cancer blocks were deparaffinized, stained with nuclear (TO-PRO-3)...
<h3>Background</h3> The complexity of the tumor microenvironment (TME) has been a major barrier in understanding response to therapy solid tumors. TME analysis traditionally based on thin 4 µm tissue sections, which represent less than 0.1% typical FFPE block. Recent literature suggests conventional histology techniques are insufficient degree immune infiltration TME. Here, we demonstrate how 3D imaging and whole samples can be used identify quantitate cytotoxic (CD8+) T-cells human cancer...
2616 Background: Non-small cell lung cancer (NSCLC) tissue is a valuable resource for diagnosis, treatment planning, and drug development. Current 2D histopathological techniques introduce under-sampling error (i.e., single 5 µm section represents 0.5% of 1 mm thick biopsy), interobserver variability, failure to capture the biology contained within entire sample. We have developed suite technologies stain, chemically clarify, image, visualize, analyze entire, intact samples. Herein, we...
<h3>Background</h3> Analysis of the three-dimensional (3D) landscape tumor microenvironment (TME) has potential to transform diagnosis and drug development. Current 2D histology techniques introduce sampling error, interobserver variability, fail capture biology contained within entire tissue. To-date, practical barriers have hampered widespread adoption 3D imaging quantification. We developed a suite technologies, Alpenglow Biosciences Aurora™ Spatial Biology platform, stain, chemically...
The numbers of immunoglobulin (Ig) containing cells in jejunal mucosa were determined 29 patients with untreated coeliac disease and 28 control subjects. had significantly increased IgM cells. Seven the studied again after 12 months on a gluten-free diet showed significant reduction number increase noted is therefore likely to be manifestation rather than primary abnormality aetiological significance.