A5040934401- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Photoacoustic and Ultrasonic Imaging
- Neurobiology and Insect Physiology Research
- Multiple Sclerosis Research Studies
- Animal Genetics and Reproduction
- Optical Imaging and Spectroscopy Techniques
- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Near-Field Optical Microscopy
- Neural dynamics and brain function
- Macrophage Migration Inhibitory Factor
- Microtubule and mitosis dynamics
- Genetics and Physical Performance
- Digital Holography and Microscopy
- Photonic and Optical Devices
- Neuroinflammation and Neurodegeneration Mechanisms
- bioluminescence and chemiluminescence research
- Lipid Membrane Structure and Behavior
- Adipose Tissue and Metabolism
- Orbital Angular Momentum in Optics
- CRISPR and Genetic Engineering
- Neuroendocrine regulation and behavior
- Congenital heart defects research
- Religion, Society, and Development
Mount Desert Island Biological Laboratory
2020-2025
Medical University of Vienna
2015-2022
TU Wien
2016-2020
A tailored protocol combines pigment removal with clearing, facilitating labeling and whole-body imaging across phylogeny.
The fruit fly, Drosophila melanogaster, is an important experimental model to address central questions in neuroscience at organismic level. However, imaging of neural circuits intact flies limited due structural properties the cuticle. Here we present a novel approach combining tissue clearing, ultramicroscopy, and data analysis that enables visualisation neuronal networks with single-cell resolution from larval stage up adult Drosophila. FlyClear, signal preserving clearing technique...
Neuromyelitis optica/spectrum disorder (NMO/SD) is a severe, inflammatory disease of the central nervous system (CNS). In majority patients, it associated with presence pathogenic serum autoantibodies (the so-called NMO-IgGs) directed against water channel aquaporin 4 (AQP4), and formation large, astrocyte-destructive lesions in spinal cord optic nerves. A large number recent studies using optical coherence tomography (OCT) demonstrated that damage to nerves NMO/SD also retinal injury, as...
Abstract We developed a deconvolution software for light sheet microscopy that uses theoretical point spread function, which we derived from model of image formation in microscope. show this approach provides excellent blur reduction and enhancement fine details stacks recorded with low magnification objectives relatively high NA field numbers as e.g. 2x 0.14 FN 22, or 4x 0.28 22. For these objectives, are widely used microscopy, sufficiently resolved functions suitable difficult to measure...
Abstract The lateral septum (LS) has been implicated in the regulation of locomotion. Nevertheless, neurons synchronizing LS activity with brain’s clock suprachiasmatic nucleus (SCN) remain unknown. By interrogating molecular, anatomical and physiological heterogeneity dopamine periventricular (PeVN; A14 catecholaminergic group), we find that Th + / Dat1 cells from its anterior subdivision innervate mice. These receive dense neuropeptidergic innervation SCN. Reciprocal viral tracing...
Abstract Primary body axis development is a highly conserved process that proceeds through somitogenesis and further subdivision into dermatome, myotome, sclerotome. Defects in somitic-clock genes such as Hes7 lead to vertebral segmentation defects mice fish. Here we show the axolotl, also necessary for proper embryonic but is, surprisingly, dispensable during axolotl tail regeneration. Using genetic barcoding fate mapping found regeneration, somitic derivatives (muscle, cartilage, tendon,...
ABSTRACT The degenerative loss of muscle associated with aging leading to muscular atrophy is called sarcopenia. Currently, practicing regular physical exercise the only efficient way delay sarcopenia onset. Identification therapeutic targets alleviate symptoms requires in vivo model organisms accelerated degeneration and atrophy. zebrafish undergoes aging, hallmarks including mitochondrial dysfunction, telomere shortening, accumulation senescent cells. However, age slowly, no specific...
We present an overview of the ultramicroscopy technique we developed. Starting from developments 100 years ago, designed a light sheet microscope and chemical clearing to image complete mouse brains. Fluorescence green fluorescent protein (GFP)-labeled neurons in brains could be preserved with our 3DISCO high-resolution three-dimensional (3-D) recordings were obtained. Ultramicroscopy was also used whole embryos flies. improved optical sectioning by generating longer thinner sheets aspheric...
Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need user-friendly system that can address applications with varied requirements in terms resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), compatibility tissue-clearing protocols holders various refractive indices. We present ‘hybrid’ combines novel non-orthogonal dual-objective conventional (orthogonal)...
Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need user-friendly system that can address applications with varied requirements in terms resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), compatibility tissue-clearing protocols holders various refractive indices. We present ‘hybrid’ combines novel non-orthogonal dual-objective conventional (orthogonal)...
Significance Daily biological rhythms are fundamental, evolutionarily conserved features of many symbioses. Here we use the binary squid–vibrio association, simplicity which has offered resolution to explore strategies symbiosis that shared with more complex systems, such as those mammals. We demonstrate a pivotal role for an cytokine, macrophage migration inhibitory factor, or MIF, is abundant in epithelia supporting symbionts both squid light organ and mammalian gut. In mature MIF...
We developed an open-source deconvolution software that stunningly increases the visibility of minute details, as for example, neurons or nerve fibers in light-sheet microscopy confocal data by combining rolling ball background subtraction three directions with using a synthetic measured point spread function. Via automatic block-wise processing image stacks virtually unlimited size can be deconvolved even on small computers 8 16 GB RAM. By parallelization and optional GPU-acceleration,...
Based on the modal analysis method, we developed a model that describes output beam of diode-pumped solid state (DPSS) laser emitting multimode beam. Measuring profile in near field and at constructed far individual modes, their respective contributions, optical parameters are determined. Using this information, is optically reshaped into quasi-Gaussian by interference superposition various modes. This process controlled mode modulator unit includes different meso-aspheric elements...
Abstract Here, we present an optically optimized system for static ultramicroscopy imaging technique. The unit generating ultra‐thin light sheet employs aspheric and meso‐optical elements (meso‐aspheric system). An analytical as well experimental comparison between the produced by standard (using a rectangular slit aperture one cylindrical lens) our latest system, which converts symmetrical Gaussian beam into is presented. Using new in combination with objective equipped modulator to...
By breaking the diffraction limit of light sheets low numerical aperture (NA) we were able to generate extremely long thin with a thickness in one micron range and vastly increased Rayleigh range. We measured different methods including standard point spread function measurement beads. using these our ultramicroscope fast 3D imaging whole mouse brains objectives large field view was possible. Due divergence could be reconstructed from single stack optical sections isotropic resolution. The...