A5071988965- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Photoacoustic and Ultrasonic Imaging
- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- Advanced Electron Microscopy Techniques and Applications
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- 3D Printing in Biomedical Research
- Memory and Neural Mechanisms
- Digital Holography and Microscopy
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Alzheimer's disease research and treatments
- Laser Applications in Dentistry and Medicine
- Ion channel regulation and function
- Congenital heart defects research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cannabis and Cannabinoid Research
- Axon Guidance and Neuronal Signaling
- Parasites and Host Interactions
TU Wien
2013-2025
University of Vienna
2009-2022
Medical University of Vienna
2011-2022
University of Applied Sciences Technikum Wien
2012-2017
Cold Spring Harbor Laboratory
2013
Max Planck Institute of Psychiatry
1999-2010
Max Planck Society
1996-2010
BioElectronics (United States)
2009-2010
University of Rome Tor Vergata
1996
Heidelberg University
1982-1983
Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence GFP expressing specimens, so that has compromise between quality preservation. This can be severe drawback when working with specimens exhibiting low expression rates. Thus, we screened for substitute found dibenzyl ether (phenylmethoxymethylbenzene, CAS 103-50-4) applied as more GFP-friendly...
It is widely accepted that cannabinoids regulate GABA release by activation of cannabinoid receptor type 1 (CB1). Results obtained from a variety brain regions consistently indicate agonists can also reduce glutamatergic synaptic transmission. However, there are still conflicting data concerning the role CB1 in cannabinoid-induced inhibition transmission cortical areas. Here, we provide direct evidence on terminals principal neurons controls excitatory responses forebrain. In slices...
A tailored protocol combines pigment removal with clearing, facilitating labeling and whole-body imaging across phylogeny.
The fruit fly, Drosophila melanogaster, is an important experimental model to address central questions in neuroscience at organismic level. However, imaging of neural circuits intact flies limited due structural properties the cuticle. Here we present a novel approach combining tissue clearing, ultramicroscopy, and data analysis that enables visualisation neuronal networks with single-cell resolution from larval stage up adult Drosophila. FlyClear, signal preserving clearing technique...
Here, we describe a novel approach that allows pathologists to three-dimensionally analyse malignant tissues, including the tumour-host tissue interface. Our visualization technique utilizes combination of ultrafast chemical clearing and light-sheet microscopy obtain virtual slices 3D reconstructions up multiple centimetre sized tumour resectates. For tumours propose preparation comprising three steps: (a) Fixation enhancement autofluorescence with formalin/5-sulfosalicylic acid. (b)...
Abstract Ultramicroscopy is a microscopical technique that allows optical sectioning and 3D reconstruction of biological medical specimens. While in confocal microscopy specimen size limited to several hundred micrometers at best, using ultramicroscopy even centimeter sized objects like whole mouse embryos can be reconstructed with micrometer resolution. This possible by combination clearing procedure the principle lightsheet illumination. We present ultramicroscopic reconstructions...
As recently shown, ultramicroscopy (UM) allows 3D-visualization of even large microscopic structures with microm resolution. Thus, it can be applied to anatomical studies numerous biological and medical specimens. We reconstructed the three-dimensional architecture tomato-lectin (Lycopersicon esculentum) stained vascular networks by UM in whole mouse organs. The topology filigree branches microvasculature was visualized. Since tumors require an extensive growth blood vessels survive, this...
Ephrin signaling through Eph receptor tyrosine kinases regulates important morphogenetic events during development and synaptic plasticity in the adult brain. Although Eph-ephrin endocytosis is required for repulsive axon guidance, its role postnatal brain unknown. Here, we show that Rin1, a brain-specific Rab5-GEF, coexpressed with EphA4 excitatory neurons interacts synaptosomal fractions. The interaction of Rin1 requires Rin1's SH2 domain, consistent view targets phosphorylated receptors...
Abstract We developed a deconvolution software for light sheet microscopy that uses theoretical point spread function, which we derived from model of image formation in microscope. show this approach provides excellent blur reduction and enhancement fine details stacks recorded with low magnification objectives relatively high NA field numbers as e.g. 2x 0.14 FN 22, or 4x 0.28 22. For these objectives, are widely used microscopy, sufficiently resolved functions suitable difficult to measure...
Abstract The lateral septum (LS) has been implicated in the regulation of locomotion. Nevertheless, neurons synchronizing LS activity with brain’s clock suprachiasmatic nucleus (SCN) remain unknown. By interrogating molecular, anatomical and physiological heterogeneity dopamine periventricular (PeVN; A14 catecholaminergic group), we find that Th + / Dat1 cells from its anterior subdivision innervate mice. These receive dense neuropeptidergic innervation SCN. Reciprocal viral tracing...
Abstract The distribution of glutamate receptor subtypes on the surface neurons is highly relevant for synaptic activation and signal processing in neocortex. As a novel approach we have used infra‐red videomicroscopy combination with photostimulation or microiontophoresis brain slices rat neocortex to map N ‐methyl‐ d ‐aspartate (NMDA) α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) receptors pyramidal layer V. Both modes application revealed spatially distinct subtypes: soma...
Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part alcohol.However, prolonged exposure to this mixture markedly reduces the fluorescence GFP expressing specimens, so that has compromise between quality preservation.This can be severe drawback when working with specimens exhibiting low expression rates.Thus, we screened for substitute found dibenzyl ether (phenylmethoxymethylbenzene, CAS 103-50-4) applied as more GFP-friendly...
Using a combination of achromatic aspheric optical elements and cylindrical lenses, we developed an improved laser light sheet generator for two-side illumination ultramicroscopy. This has much longer Rayleigh range, more uniform spatial intensity distribution along x -, y - z-axis, reduced aberrations than the standard system consisting slit aperture single lens, which is commonly used in microscopy. As there no truncation beam by our design energy efficiently. Applying this to...