A5077985931- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Inflammatory Bowel Disease
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Immune cells in cancer
- Microscopic Colitis
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Research on Leishmaniasis Studies
- Tuberculosis Research and Epidemiology
- Eosinophilic Esophagitis
- Ferroptosis and cancer prognosis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Image Analysis Techniques
- Mycobacterium research and diagnosis
- Cell Adhesion Molecules Research
- Advanced Fluorescence Microscopy Techniques
- Viral Infections and Vectors
- Epigenetics and DNA Methylation
- IL-33, ST2, and ILC Pathways
- Trypanosoma species research and implications
- Bone Metabolism and Diseases
- Sphingolipid Metabolism and Signaling
Amgen (United States)
2016-2024
Gilead Sciences (United States)
2023-2024
PCR Oncology
2023
Genentech
2014-2015
National Institute of Allergy and Infectious Diseases
2006-2014
National Institutes of Health
2005-2014
Laboratory of Molecular Genetics
2009
Center for Biologics Evaluation and Research
2008
United States Food and Drug Administration
2008
University of California, Berkeley
2001-2004
Infection with the obligate intracellular protozoan Leishmania is thought to be initiated by direct parasitization of macrophages, but early events following transmission skin vector sand flies have been difficult examine directly. Using dynamic intravital microscopy and flow cytometry, we observed a rapid sustained neutrophilic infiltrate at localized fly bite sites. Invading neutrophils efficiently captured major (L.m.) parasites after or needle inoculation, phagocytosed L.m. remained...
In contrast to naïve T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily intact proteins. How and where within a lymph node such unprocessed antigens become available for cell recognition is not clear. We used two-photon intravital imaging show that, after exiting high-endothelial venules before entry into follicles, survey locally concentrated dendritic cells. Engagement the receptor...
Etrolizumab is a humanised monoclonal antibody that selectively binds the β7 subunit of heterodimeric integrins α4β7 and αEβ7. We aimed to assess etrolizumab in patients with moderately-to-severely active ulcerative colitis.In this double-blind, placebo-controlled, randomised, phase 2 study, colitis who had not responded conventional therapy were recruited from 40 referral centres 11 countries. Eligible (aged 18-75 years; Mayo Clinic Score [MCS] 5 higher [or ≥6 USA]; disease extending 25 cm...
Increasing evidence suggests that asthma is a heterogeneous disorder regulated by distinct molecular mechanisms. In cross-sectional study of asthmatics varying severity (n = 51), endobronchial tissue gene expression analysis revealed three major patient clusters: TH2-high, TH17-high, and TH2/17-low. TH2-high TH17-high patterns were mutually exclusive in individual samples, their signatures inversely correlated differentially interleukin-13 (IL-13) IL-17A. To understand this dichotomous...
<h3>Background</h3> There is microscopic spatial and temporal heterogeneity of pathological changes in idiopathic pulmonary fibrosis (IPF) lung tissue, which may relate to pathophysiological mediators disease clinical progression. We assessed relationships between gene expression patterns, features, systemic biomarkers identify that reflect the aggregate burden patients with IPF. <h3>Methods</h3> Gene microarrays (N=40 IPF; 8 controls) immunohistochemical analyses (N=22 biopsies. Clinical...
Abstract Dectin-1 is a fungal pattern recognition receptor that binds to β-glucans and triggers cytokine production by facilitating interaction with TLR2 or directly activating spleen tyrosine kinase (Syk). To assess the possible role of in innate response mycobacteria, we used an vitro system which IL-12p40 measured splenic dendritic cells (SpDC) following exposure live Mycobacterium tuberculosis bacilli. Treatment SpDC laminarin glucan phosphate, two molecules known block...
Leishmaniasis is transmitted between mammalian hosts by the bites of bloodsucking vector sand flies. The dose parasites to host has never been directly determined. We developed a real-time PCR-based method determine number Leishmania major inoculated into ears living mice during feeding individual infected flies ( Phlebotomus duboscqi ). varied over wide range in 58 which were detected and demonstrated clear bimodal distribution. Most with low <600 parasites. One four received higher...
<h3>Objective</h3> Etrolizumab (rhuMAb β7, anti-β7, PRO145223) is a humanised monoclonal antibody targeting the β7 subunit of heterodimeric integrins α4β7 and αEβ7, which are implicated in leucocyte migration retention ulcerative colitis (UC). This randomised phase I study evaluated safety pharmacology etrolizumab patients with moderate to severe UC. <h3>Design</h3> In single ascending dose (SAD) stage, (0.3, 1.0, 3.0, 10 mg/kg intravenous, 3.0 subcutaneous (SC) or placebo) was administered...
Etrolizumab is a humanized monoclonal antibody against the β7 integrin subunit that has shown efficacy vs placebo in patients with moderate to severely active ulcerative colitis (UC). Patients colon tissues expressed high levels of αE gene (ITGAE) appeared have best response. We compared differences colonic expression ITGAE and other genes between who achieved clinical remission etrolizumab those did.We performed retrospective analysis data collected from 110 UC participated phase 2...
The lung is a relatively quiescent organ during homeostasis but has remarkable capacity for repair after injury. Alveolar epithelial type I cells (AEC1s) line airspaces and mediate gas exchange. After injury, they are regenerated by differentiation from their progenitors — alveolar II (AEC2s) which also secrete surfactant to maintain surface tension patency. While recent studies showed that the maintenance of AEC2 stemness Wnt dependent, molecular mechanisms underlying AEC2-AEC1 in adult...