A5063887566- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune cells in cancer
- NF-κB Signaling Pathways
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Genomics and Chromatin Dynamics
- Chemical Reactions and Mechanisms
- Enzyme function and inhibition
- Cancer Immunotherapy and Biomarkers
- Psoriasis: Treatment and Pathogenesis
- Peroxisome Proliferator-Activated Receptors
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Electrochemical Analysis and Applications
- DNA and Nucleic Acid Chemistry
- Amino Acid Enzymes and Metabolism
- RNA modifications and cancer
- Molecular Junctions and Nanostructures
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Adipokines, Inflammation, and Metabolic Diseases
University of California, Riverside
2023
Amgen (United States)
2020-2022
Columbia University
2014-2021
University of California, Los Angeles
2009-2013
The expression of eukaryotic mRNAs is achieved though an intricate series molecular processes that provide many steps for regulating the production a final gene product. However, relationships between individual in mRNA biosynthesis and rates at which they occur are poorly understood. By applying RNA-seq to chromatin-associated soluble nucleoplasmic fractions RNA from Lipid A-stimulated macrophages, we examined timing exon ligation transcript release chromatin relative induction...
Abstract Background Elucidation of immune populations with single-cell RNA-seq has greatly benefited the field immunology by deepening characterization heterogeneity and leading to discovery new subtypes. However, methods inherently suffer from limitations in recovery complete transcriptomes due prevalence cellular transcriptional dropout events. This issue is often compounded limited sample availability prior knowledge heterogeneity, which can confound data interpretation. Results Here, we...
Abstract Metformin is a first-line drug in the treatment of type-2 diabetes mellitus (T2DM). In addition to its antigluconeogenic and insulin-sensitizing properties, metformin has emerged as potent inhibitor chronic inflammatory response macrophages. particular, been shown reduce expression interleukin (IL-) 1β during long-term exposure pro-inflammatory stimulus lipopolysaccharide (LPS) through reduction reactive oxygen species (ROS), which decreases levels hypoxia-inducible factor (HIF)...
Serine / arginine (SR) protein kinases (SRPKs) are capable of transmitting external signals to the spliceosome by phosphorylating SR proteins. In plants, few studies have looked at regulation RNA splicing post-translational modification (PTMs), despite and factor proteins exhibiting extensive phosphorylation. Many these PTM events potential dramatically change expression various genes, such as those involved in abiotic stress. Here, we sought explore regulatory function Arabidopsis thaliana...
Single-cell RNA sequencing is a powerful tool to examine cellular heterogeneity, novel markers and target genes, therapeutic mechanisms in human cancers animal models. Here, we analyzed single-cell data of T cells obtained from multiple mouse tumor models by PCA-based subclustering coupled with TCR tracking using the STARTRAC algorithm. This approach revealed various differentiated cell subsets activation states, correspondence between tumors. analyses demonstrated peripheral that were...
The importance of electrochemical analysis for charge-transfer science cannot be overstated. Interfaces in cells present certain challenges the interpretation and utility analysis. This publication focuses on: (1) medium polarity that redox species experience at electrode surfaces is smaller than bulk media (2) liquid-junction potentials from interfacing electrolyte solutions different organic solvents, namely, dichloromethane, benzonitrile, acetonitrile. Electron-donor-acceptor pairs...
Abstract Liver metastasis is associated with immunotherapy resistance, although the underlying mechanisms remain incompletely understood. By applying single cell RNA‐sequencing to a concurrent subcutaneous and liver tumor murine model recapitulate metastases, it identified that subsets within tumor‐infiltrating exhausted CD8 + T (Tex) cells immunosuppressive tumor‐associated macrophages (TAMs) display opposite responses tumors anti‐PD‐1 treatment, suggesting complex immune regulating...
Virtually all living organisms have evolved mechanisms to adapt their environment by sensing environmental stresses and inducing the transcription of appropriate sets response genes in a coordinated fashion. In vertebrate immune system, highly selective an stimulus, often invading microorganism, plays especially important role regulating activities of, interactions among, many cell types involved innate adaptive immunity. It is now widely appreciated that stimulus requires concerted action...
Abstract Background: Elucidation of immune populations with single-cell RNA-seq has greatly benefited the field immunology by deepening characterization heterogeneity and leading to discovery new subtypes. However, methods inherently suffer from limitations in recovery complete transcriptomes due prevalence cellular transcriptional dropout events. This issue is often compounded limited sample availability prior knowledge heterogeneity, which can confound data interpretation. Results: Here,...
Abstract Background Elucidation of immune populations with single-cell RNA-seq has greatly benefited the field immunology by deepening characterization heterogeneity and leading to discovery new subtypes. However, methods inherently suffer from limitations in recovery complete transcriptomes due prevalence cellular transcriptional dropout events. This issue is often compounded limited sample availability prior knowledge heterogeneity, which can confound data interpretation. Results Here, we...
Abstract Background : Elucidation of immune populations with single-cell RNA-seq has greatly benefited the fieldof immunology by deepening characterization heterogeneity and leading to thediscovery new subtypes. However, methods inherently suffer from limitations in therecovery complete transcriptomes due prevalence cellular transcriptional dropoutevents. This issue is often compounded limited sample availability prior knowledge ofheterogeneity, which can confound data interpretation....
Abstract Background: Elucidation of immune populations with single-cell RNA-seq has greatly benefited the field immunology by deepening characterization heterogeneity and leading to discovery new subtypes. However, methods inherently suffer from limitations in recovery complete transcriptomes due prevalence cellular transcriptional dropout events. This issue is often compounded limited sample availability prior knowledge heterogeneity, which can confound data interpretation. Results: Here,...