A5072229921- Advanced Fluorescence Microscopy Techniques
- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Cell Image Analysis Techniques
- Advanced Radiotherapy Techniques
- Photoacoustic and Ultrasonic Imaging
- Medical Imaging Techniques and Applications
- Molecular Biology Techniques and Applications
- Optical Imaging and Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Radiation Detection and Scintillator Technologies
- Optical Coherence Tomography Applications
- Advanced X-ray and CT Imaging
- Prostate Cancer Diagnosis and Treatment
- Radiation Therapy and Dosimetry
- Single-cell and spatial transcriptomics
- Nanoplatforms for cancer theranostics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photodynamic Therapy Research Studies
- Radiation Dose and Imaging
- Advanced Biosensing Techniques and Applications
- Breast Cancer Treatment Studies
- Advanced NMR Techniques and Applications
- Digital Radiography and Breast Imaging
- Solid-state spectroscopy and crystallography
Allen Institute
2022-2025
Allen Institute for Neural Dynamics
2022-2025
University of Washington
2015-2024
Allen Institute for Brain Science
2023
Georgia Institute of Technology
2022
Emory University
2022
Case Western Reserve University
2022
Dartmouth College
2011-2017
Dartmouth–Hitchcock Medical Center
2012-2015
University of Pennsylvania
2015
Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural molecular information from intact tissues. However, current microscopes imposed constraints on the size, shape, number of specimens, compatibility with various protocols. Here we present a multi-immersion open-top microscope that enables simple mounting multiple specimens processed variety protocols, which will facilitate wide adoption by preclinical researchers clinical...
Recent studies have proposed that light emitted by the Cherenkov effect may be used for a number of radiation therapy dosimetry applications. There is correlation between captured and expected dose under certain conditions, yet discrepancies also been observed complete examination theoretical differences has not done. In this study, fundamental comparison emission absorbed was explored x-ray photons, electrons, protons using both Monte Carlo-based analysis. Based on findings where correlates...
Abstract Intraoperative identification of carcinoma at lumpectomy margins would enable reduced re-excision rates, which are currently as high 20% to 50%. Although imaging disease-associated biomarkers can identify malignancies with specificity, multiplexed such is necessary detect molecularly heterogeneous carcinomas sensitivity. We have developed a Raman-encoded molecular (REMI) technique in targeted nanoparticles topically applied on excised tissues rapid visualization panel cell surface...
Fluorescence microscopy is a workhorse tool in biomedical imaging but often poses substantial challenges to practitioners achieving bright or uniform labeling. In addition, while antibodies are effective specific labels, their reproducibility inconsistent, and they difficult use when staining thick specimens. We report the of conventional, commercially available fluorescent dyes for rapid intense covalent labeling proteins carbohydrates super-resolution (expansion) cleared tissue microscopy....
Prostate cancer treatment planning is largely dependent upon examination of core-needle biopsies. The microscopic architecture the prostate glands forms basis for prognostic grading by pathologists. Interpretation these convoluted three-dimensional (3D) glandular structures via visual inspection a limited number two-dimensional (2D) histology sections often unreliable, which contributes to under- and overtreatment patients. To improve risk assessment decisions, we have developed workflow...
Purpose: A novel technique for beam profiling of megavoltage photon beams was investigated the first time by capturing images induced Čerenkov emission in water, as a potential surrogate imparted dose irradiated media. Methods: high‐sensitivity, intensified CCD camera (ICCD) configured to acquire 2D projection from 4 × cm 2 6 MV linear accelerator (LINAC) x‐ray operating at rate 400 MU/min incident on water tank with transparent walls. The ICCD acquisition gated LINAC sync pulse reduce...
Full 3D beam profiling and quality assurance (QA) of therapeutic megavoltage linear accelerator (LINAC) x-ray photon beams is not routinely performed due to the slow point-by-point measurement nature conventional scanning ionization chamber systems. In this study we explore a novel optical-based dose imaging approach using standard commercial camera, water tank, fluorescent dye, which when excited by Čerenkov emission induced radiation beam, allows 2D projection in fast timeframe,...
Since its discovery during the 1930s Čerenkov effect (light emission from charged particles traveling faster than local speed of light in a dielectric medium) has been paramount development high-energy physics research. The ability emitted to describe particle's trajectory, energy, velocity, and mass allowed scientists study subatomic particles, detect neutrinos, explore properties interstellar matter. However, our knowledge, all applications process date have focused on identification...
Radiation from a linear accelerator induces Cherenkov emission in tissue, which has recently been shown to produce biochemical spectral signatures that can be interpreted estimate tissue hemoglobin and oxygen saturation or molecular fluorescence reporters. The optical light levels are the range of 10(-6) 10(-9) W/cm2, limits practical utility signal routine radiation therapy monitoring. However, due fact is pulsed, gated-acquisition allows detection presence ambient lighting, as demonstrated...
Cherenkov radiation has recently emerged as an interesting phenomenon for a number of applications in the biomedical sciences. Its unique properties, including broadband emission spectrum, spectral weight ultraviolet and blue wavebands, local generation light within given tissue, have made it attractive new source tissue molecular imaging phototherapy applications. While several studies investigated total yield from radionuclides units [photons/decay], further consideration propagation is...
Purpose: Čerenkov radiation emission occurs in all tissue, when charged particles (either primary or secondary) travel at velocity above the threshold for effect (about 220 KeV tissue electrons). This study presents first examination of optical as a surrogate absorbed superficial dose MV x‐ray beams. Methods: In this study, Monte Carlo simulations flat and curved surfaces were studied to analyze energy spectra produced different regions near irradiated by intensity directly simulated...
Open-top light-sheet microscopy is a technique that can potentially enable rapid ex vivo inspection of large tissue surfaces and volumes.Here, we have optimized an open-top (OTLS) microscope image-processing workflow for the comprehensive examination surgical margin surfaces, also developed novel fluorescent analog H&E staining robust fresh unfixed tissues.Our tissue-staining method be achieved within 2.5 minutes followed by OTLS lumpectomy at rate up to 1.5 cm 2 /minute.An image atlas...
Abstract Prostate cancer treatment decisions rely heavily on subjective visual interpretation [assigning Gleason patterns or International Society of Urological Pathology (ISUP) grade groups] limited numbers two‐dimensional (2D) histology sections. Under this paradigm, interobserver variance is high, with ISUP grades not correlating well outcome for individual patients, and contributes to the over‐ undertreatment patients. Recent studies have demonstrated improved prognostication prostate...
Čerenkov emission is generated from ionizing radiation in tissue above 264 keV energy. This study presents the first examination of this optical as a surrogate for absorbed superficial dose. was imaged surface flat phantoms irradiated with electrons, using range field sizes 6 cm × to 20 cm, incident angles 0° 50°, and energies 18 MeV. The images were compared estimated dose direct diode measurements, well calculations by Monte Carlo treatment planning system. Intensity showed outstanding...
We describe a tissue optics plug-in that interfaces with the GEANT4/GAMOS Monte Carlo (MC) architecture, providing means of simulating radiation-induced light transport in biological media for first time. Specifically, we focus on simulation due to Čerenkov effect (light emission from charged particle's traveling faster than local speed given medium), phenomenon which requires accurate modeling both high energy particle and subsequent optical photon transport, dynamic coupled process is not...
Intraoperative assessment of breast surgical margins will be value for reducing the rate re-excision surgeries lumpectomy patients. While frozen-section histology is used intraoperative guidance certain cancers, it provides limited sampling margin surface (typically <1 % margin) and inferior to gold-standard histology, especially fatty tissues that do not freeze well, such as specimens. Microscopy with ultraviolet excitation (MUSE) a nondestructive superficial optical-sectioning technique...
Ionizing radiation is commonly delivered by medical linear accelerators (LINAC) in the form of shaped beams, and it able to induce Cherenkov emission tissue. In fluorescence-based microscopy excitation from scanned spots, lines, or sheets can be used for fast high-resolution imaging. Here we introduce Cherenkov-excited luminescence imaging (CELSI) as a new methodology utilizing 2-dimensional (∼5-mm-thick) LINAC produce photons, which turn excite probes distributed biological tissues. Imaging...
Ex vivo microscopy encompasses a range of techniques to examine fresh or fixed tissue with microscopic resolution, eliminating the need embed in paraffin produce glass slide. One such technique is light-sheet microscopy, which enables rapid 3D imaging. Our pathology-engineering collaboration has resulted an open-top (OTLS) microscope that specifically tailored needs pathology practice.To present image atlas OTLS images prostate core needle biopsies.Core biopsies (N = 9) were obtained from...
Open-top light-sheet (OTLS) microscopes have been developed for user-friendly and versatile high-throughput 3D microscopy of thick specimens. As with all imaging modalities, spatial resolution trades off analysis times. A hierarchical multi-scale workflow would therefore be value many volumetric applications. We describe a compact multi-resolution OTLS microscope, enabled by novel solid immersion meniscus lens (SIMlens), which allows users to rapidly transition between air-based objectives...