A5028635627- MRI in cancer diagnosis
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
- AI in cancer detection
- Medical Malpractice and Liability Issues
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cancer Genomics and Diagnostics
- Patient Safety and Medication Errors
- Nanoplatforms for cancer theranostics
- Breast Cancer Treatment Studies
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Clinical Reasoning and Diagnostic Skills
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced MRI Techniques and Applications
- Innovations in Medical Education
- Medical Imaging Techniques and Applications
- Biochemical Analysis and Sensing Techniques
- 3D Printing in Biomedical Research
- Olfactory and Sensory Function Studies
- Advanced Fluorescence Microscopy Techniques
- Radiology practices and education
- Neuroscience of respiration and sleep
University of Washington
2013-2024
Fred Hutch Cancer Center
2023-2024
University of Washington Medical Center
2010-2019
Seattle University
2011-2019
Blaze Bioscience (United States)
2018
Hadassah Medical Center
2018
Harvard University
2018
Overlake Hospital Medical Center
2018
American Journal Experts (United States)
2015
American Society for Clinical Pathology
2015
Recent years have seen development and implementation of anticancer therapies targeted to particular gene mutations, but methods assay clinical cancer specimens in a comprehensive way for the critical mutations remain underdeveloped. We developed UW-OncoPlex, molecular diagnostic provide simultaneous deep-sequencing information, based on >500× average coverage, all classes 194 clinically relevant genes. To validate we tested 98 previously characterized tumor from 10 different types,...
There is a need to image excised tissues during tumor-resection procedures in order identify residual tumors at the margins and guide their complete removal. The imaging of dysregulated cell-surface receptors potential means identifying presence diseases with high sensitivity specificity. However, due heterogeneities expression protein biomarkers tumors, molecular-imaging technologies should ideally be capable visualizing multiplexed panel cancer biomarkers. Here, we demonstrate that topical...
Abstract Intraoperative identification of carcinoma at lumpectomy margins would enable reduced re-excision rates, which are currently as high 20% to 50%. Although imaging disease-associated biomarkers can identify malignancies with specificity, multiplexed such is necessary detect molecularly heterogeneous carcinomas sensitivity. We have developed a Raman-encoded molecular (REMI) technique in targeted nanoparticles topically applied on excised tissues rapid visualization panel cell surface...
In 2009, a College of American Pathologists expert panel published supplemental HER2 testing recommendations suggesting that cases with between 5% and 50% individual cells amplified by fluorescence in situ hybridization be reported as "heterogeneous for gene amplification." We examined the implications applying these to clinical practice 1,329 consecutive breast cancer cases. By ratio criteria, 23.2% met proposed criteria heterogeneity, which 81.6% were not 15.5% equivocal standard criteria....
Open-top light-sheet microscopy is a technique that can potentially enable rapid ex vivo inspection of large tissue surfaces and volumes.Here, we have optimized an open-top (OTLS) microscope image-processing workflow for the comprehensive examination surgical margin surfaces, also developed novel fluorescent analog H&E staining robust fresh unfixed tissues.Our tissue-staining method be achieved within 2.5 minutes followed by OTLS lumpectomy at rate up to 1.5 cm 2 /minute.An image atlas...
To explore the molecular basis of X chromosome inactivation, we have examined human locus for glucose-6-phosphate dehydrogenase (G6PD) in various tissues. Studies DNA from males and females somatic cell hybrids with active or inactive chromosomes, show that two remarkably dense clusters CpG dinucleotides 3′ coding sequences are hypomethylated G6PD genes but extensively methylated ones. Reacquisition activity, either spontaneous induced by 5-azacytidine, is accompanied demethylation both...
Intraoperative assessment of breast surgical margins will be value for reducing the rate re-excision surgeries lumpectomy patients. While frozen-section histology is used intraoperative guidance certain cancers, it provides limited sampling margin surface (typically <1 % margin) and inferior to gold-standard histology, especially fatty tissues that do not freeze well, such as specimens. Microscopy with ultraviolet excitation (MUSE) a nondestructive superficial optical-sectioning technique...
Calcifications play an essential role in early breast cancer detection and diagnosis. However, information regarding the chemical composition of calcifications identified on mammography histology is limited. Detailed spectroscopy reveals association between cancer, warranting development novel analytical tools to better define calcification types. Previous investigations average across broad tissue sections with no spatially resolved or provide qualitative visualization, which prevents a...
We were interested in determining our concordance between fluorescence situ hybridization (FISH) and a previously validated immunohistochemical HER2 assay to identify possible reasons for discordance determine if all addressed by the American Society of Clinical Oncology/College Pathologists guidelines. reviewed 697 cases (2004-2007) which FISH testing concurrently done. Overall nonequivocal results was 96%. Of 19 discordant cases, 13 (68%) interpreted as positive immunohistochemically but...
Deregulation of DNA repair enzymes occurs in cancers and may create a susceptibility to chemotherapy. Expression levels have been shown predict the responsiveness certain chemotherapeutic agents. The RECQ helicases damaged including damage caused by topoisomerase I inhibitors, such as irinotecan. Altered expression these colorectal cancer (CRC) influence response Thus, we assessed helicase (WRN, BLM, RECQL, RECQL4, RECQL5) primary CRCs, matched normal colon, CRC cell lines. We found that BLM...
Physicians are urged to communicate more openly following medical errors, but little is known about pathologists' attitudes reporting errors their institution and disclosing them patients. We undertook a survey characterize laboratory directors' experience regarding the communication of with hospitals, treating physicians, affected invited 260 practicing pathologists 81 academic hospital directors participate in self-administered survey. This included questions estimated error rates barriers...
For breast cancer patients, the extent of regional lymph node (LN) metastasis influences decision to remove all axillary LNs. Metastases are currently identified and classified with visual analysis a few thin tissue sections conventional histology that may underrepresent metastases.We sought enable nondestructive three-dimensional (3D) pathology human LNs develop practical workflow for LN staging our method. We also evaluate whether 3D improves accuracy in comparison two-dimensional (2D)...
The 300-kDa mannose 6-phosphate receptor cycles between the trans Golgi network and late endosomes, plasma membrane early to deliver lysosomal enzymes prelysosomes.Mannose trafficking requires structural determinants present in cytoplasmic domain.However, when this domain was joined with extracellular transmembrane domains of epidermal growth factor receptor, it not sufficient direct chimera endosomes (Dintzis, s.M., Pfeffer, s.R. (1990) EMBO J. 9, 77-84).These findings suggested a role for...
Context.— Resection of breast carcinoma with adequate margins reduces the risk local recurrence and reoperation. Tozuleristide (BLZ-100) is an investigational peptide-fluorophore agent that may aid in intraoperative tumor detection margin assessment. In this study, fluorescence imaging was conducted ex vivo on gross pathology specimens. Objectives.— To determine potential tozuleristide to detect fresh specimens feasibility fluorescence-guided assessment surgical margins. Design.—...
Context.— Surgical specimen adverse events can lead to delays in treatment or diagnosis, misdiagnosis, reoperation, inappropriate treatment, and anxiety serious patient harm. Objectives.— To describe the types frequency of event reports associated with management surgical specimens, contributing factors, level harm these events. Design.— A retrospective review was undertaken near misses voluntarily reported University HealthSystem Consortium Safety Intelligence Patient Organization database...