Hyperspectral stimulated Raman scattering (hsSRS) microscopy has recently emerged as a powerful non-destructive technique for the label-free chemical imaging of biological samples. In most hsSRS experiments, SRS spectral range is limited by total bandwidth excitation laser to ~300 cm-1 and resolution ~25 cm-1. Here we present novel approach broadband based on parabolic fiber amplification provide linearly chirped broadened Stokes pulses. This instrument provides >600 coverage ~10 resolution....

Calcifications play an essential role in early breast cancer detection and diagnosis. However, information regarding the chemical composition of calcifications identified on mammography histology is limited. Detailed spectroscopy reveals association between cancer, warranting development novel analytical tools to better define calcification types. Previous investigations average across broad tissue sections with no spatially resolved or provide qualitative visualization, which prevents a...

Fingerprints have long been the gold standard for personal identification in forensic investigations. Methods cultivating and enhancing visualization of latent fingerprints (LFPs) are continuously evolving. One important challenge is to identify suspicious chemicals present fingerprint residues, which requires chemical imaging capability. Recently, vibrational spectroscopy has shown that LFP analysis through tape-lift, Raman mapping, multivariate data presents a useful tool investigation....

It has been estimated that approximately 50% of all marketed drug molecules are manufactured and administered in the form salts, often with goal improving solubility, dissolution rate, efficacy drug. However, salt disproportionation during processing or storage is a common adverse effect these formulations. Due to heterogeneous nature solid formulations, it essential characterize substances noninvasively at micrometer resolution understand molecular mechanism disproportionation. there lack...

Optical "virtual biopsy" is an attractive way to improve disease diagnosis and surgical guidance. Many optical microscopy techniques have been developed provide diagnostic information without the need for tissue sectioning or staining. Among these techniques, label-free chemical imaging most desirable. Recently, it has shown that narrowband, picosecond stimulated Raman scattering (SRS) can achieve comparable morphological contrast hematoxylin eosin staining (H&E staining), 'gold standard' of...

Microscale thermometry of aqueous solutions is essential to understand the dynamics local heat generation and dissipation in chemical biological systems. A wide variety fluorescent probes have been developed map temperature changes with submicrometer resolution, but they often suffer from uncertainty associated microenvironment-dependent properties. In this work, we develop a label-free ratiometric stimulated Raman scattering (SRS) microscopy technique quantify microscale by monitoring O–H...

A general technique is developed to retrieve the fractal dimension of self-similar soils through microwave (radar) scattering. The based on a mathematical model relating dimensions georadargram that scattering structure. Clear and different signatures have been observed over four geosystems (soils sediments) compared in this work.

Cell size and density are tightly controlled in mammalian cells. They impact a wide range of physiological functions, including osmoregulation, tissue homeostasis, growth regulation. Compared to size, variation for given cell type is typically much smaller, implying that cell-type-specific plays an important role function. However, little known about how affects function or it regulated. Current tools intracellular measurements limited either suspended cells grown on 2D substrates, neither...

Using a combination of laboratory experiments and computer simulation we show that microwaves reflected from transmitted through soil have fractal dimension correlated to the soil's hierarchic permittivity network. The mathematical model relating ground-penetrating radar record mass structure is also developed. signature scattered correlates well with some physical mechanical properties soils.

Here, we present a study of how liposomes are loaded and release their contents during electrochemical detection. We 200 nm with redox mediator, ferrocyanide, used amperometry to detect collision on carbon-fiber microelectrode (CFE). found that could control the favorability electroporation process amount ferrocyanide released by modifying osmolarity buffer in which were suspended. Interestingly, observed quantity varied significantly nonmonotonic fashion. Using stimulated Raman scattering...

Abstract We have shown that barium [from BaSO 4 nanoparticles (NPs)] was cleared from the lungs faster than other poorly soluble NPs and translocated mostly to bone. now studied biokinetics in rats during Study 1: two-year inhalation exposure 50 mg/m 3 NP aerosols, 2: single intratracheal (IT) instillation of increasing doses or BaCl 2 . 1 showed lung content measured by inductively coupled plasma mass spectrometry increased 360 days aerosol exposures. An equilibrium established time until...

Political parties operate as partisan social groups and partisanship biases the way individuals process political information, form opinions, evaluate candidates. Partisans demonstrate favoritism when evaluating fellow party members but discriminate against those in opposing party. However, bounds of this intergroup bias are undetermined. Using a constructed website, an experiment tested extent reach identity biasing evaluations on candidate brand attributes. That limit was not ascertained...

Abstract Cell size and density impact a wide range of physiological functions, including tissue homeostasis, growth regulation, osmoregulation. Both are tightly regulated in mammalian cells. In comparison, variation given cell type is much smaller than size, indicating that maintenance type-specific important for function. Despite this importance, little known about how affects function it controlled. Current tools intracellular measurements limited either to suspended cells or growing on 2D...

InGaAs/InAlAs quantum well infrared photodetectors have been investigated as a function of doping level. It is shown that depending on the figure merit to be optimized different level required.

Dark current noise in InGaAs/InAlAs quantum well infrared photodetectors has been investigated as a function of temperature and bias voltage. From the dependence on these parameters gain determined.

Hyperspectral Stimulated Raman scattering (hsSRS) microscopy has recently emerged as a powerful non-destructive technique for label-free chemical imaging of biological samples. In most hsSRS experiments, the SRS spectral range is limited by total bandwidth excitation laser to ~300 cm^-1 and resolution ~25 cm^-1. Here we present novel approach broadband based on parabolic fiber amplification provide linearly chirped broadened Stokes pulses. This instrument provides >600...

In most hyperspectral SRS imaging experiments, the spectral range is limited by total bandwidth of excitation laser to ~300 cm−1 and resolution ~20 cm−1. Here we present a novel approach for broadband hsSRS microscopy based on parabolic fiber amplification provide linearly chirped broadened Stokes pulses. This instrument provides >600 cm--−1 coverage ~10 resolution.