A5017824997- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- S100 Proteins and Annexins
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Pneumonia and Respiratory Infections
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Systemic Lupus Erythematosus Research
- Cancer Cells and Metastasis
- Asthma and respiratory diseases
- Reproductive System and Pregnancy
- Viral gastroenteritis research and epidemiology
- Rheumatoid Arthritis Research and Therapies
- Metabolomics and Mass Spectrometry Studies
- Immune cells in cancer
The University of Adelaide
2015-2024
University of South Australia
2021-2023
Centre for Cancer Biology
2012-2015
Institute of Molecular Pathology and Pathomorphology
2014
Max Planck Institute of Neurobiology
2010
Medizinische Hochschule Hannover
2010
Seigakuin University
2010
University of Bonn
2010
Max Planck Institute of Biochemistry
2010
Commonwealth Scientific and Industrial Research Organisation
2009
A chronic inflammatory disease may be characterized by an accumulation of activated leukocytes at the site inflammation. Since chemokine RANTES play active role in recruiting into sites, we investigated ability cultured human synovial fibroblasts isolated from patients suffering rheumatoid arthritis to produce this and compared its regulation that closely related gene, interleukin-8 (IL-8). In unstimulated fibroblasts, expression mRNA for both chemokines was undetectable, but increased a...
Abstract In this study, we have evaluated the role of specific chemotactic cytokines in leukocyte recruitment to s.c. tissue response TNF-alpha vivo. Injection into air pouches led a rapid, transient accumulation leukocytes. Maximal leukocytes pouch was observed at between 2 and 4 h after injection TNF-alpha. The cellular exudate comprised predominantly neutrophils, with smaller numbers eosinophils mononuclear phagocytes also being recruited. However, lymphocyte not observed. induced...
Abstract Macrophage migration inhibitory factor (MIF) was originally identified for its ability to inhibit the random of macrophages in vitro. MIF is now recognized as an important mediator a range inflammatory disorders. We recently observed that absence associated with reduction leukocyte-endothelial cell interactions induced by mediators, suggesting one mechanism whereby acts during responses promoting leukocyte recruitment. However, it unknown whether capable inducing recruitment...
Neutrophils, an abundant cell type at sites of inflammation, have the ability to produce a number cytokines, including interleukin 1 (IL-1), IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis alpha (TNF-alpha). In this study, we examined human neutrophils IL-1 receptor antagonist (IL-1Ra), 17-23-kD protein recently isolated cloned from macrophages. Since IL-1Ra has been shown inhibit both in vitro vivo effects IL-1, its production by large numbers...
Alphaviruses, such as chikungunya virus and Ross River (RRV), are associated with outbreaks of infectious rheumatic disease in humans worldwide. Using an established mouse model that mimicsRRVdisease humans, we showed macrophage-derived factors critical the development striated muscle joint tissue damage. Histologic analyses ankle tissues demonstrated a substantial reduction inflammatory infiltrates infected mice depleted macrophages (i.e., "macrophage-depleted mice"). Levels proinflammatory...
Type 1 regulatory T (TR1) cells are Foxp3- interleukin-10 (IL-10)-producing CD4+ with potent immunosuppressive properties, but their requirements for lineage development have remained elusive. We show that TR1 constitute the most abundant population after allogeneic bone marrow transplantation (BMT), express transcription factor Eomesodermin (Eomes), and critical prevention of graft-versus-host disease. demonstrate Eomes is required cell differentiation, during which it acts in concert B...
Abstract IL-17-producing helper T (Th17) cells are critical for host defense against extracellular pathogens but also drive numerous autoimmune diseases. Th17 that differ in their inflammatory potential have been described including IL-10-producing weak inducers of inflammation and highly inflammatory, IL-23-driven, GM-CSF/IFNγ-producing cells. However, distinct developmental requirements, functions trafficking mechanisms vivo remain poorly understood. Here we identify a temporally regulated...
MR1-restricted mucosal-associated invariant T (MAIT) cells play a unique role in the immune system. These develop intrathymically through three-stage process, but events that regulate this are largely unknown. Here, using bulk and single-cell RNA sequencing-based transcriptomic analysis mice humans, we studied changing transcriptional landscape accompanies transition each stage. Many transcripts were sharply modulated during MAIT cell development, including SLAM (signaling lymphocytic...
Abstract Interleukin 17-producing γδ T (γδT17) cells have unconventional trafficking characteristics, residing in mucocutaneous tissues but also homing into inflamed via circulation. Despite being fundamental to γδT17-driven early protective immunity and exacerbation of autoimmunity cancer, migratory cues controlling γδT17 cell positioning barrier recruitment inflammatory sites are still unclear. Here we show that constitutively express chemokine receptors CCR6 CCR2. While recruits resting...
To examine the role of chemokines, S100A8, and S100A9 in neutrophil accumulation induced by causative agent gout, monosodium urate monohydrate (MSU) crystals.MSU crystal-induced migration was studied murine air-pouch model. Release S100A9, S100A8/A9 response to MSU crystals quantified enzyme-linked immunosorbent assays. Recruited cells were counted following acetic blue staining, subpopulations characterized Wright-Giemsa staining cytospins.MSU neutrophils injection air pouch, which...
Abstract In this study, we have examined the ability of chemokine receptor antagonists to prevent neutrophil extravasation in mouse. Two murine CXC chemokines, macrophage-inflammatory protein (MIP)-2 and KC, stimulated accumulation leukocytes into s.c. air pouches, although MIP-2 was considerably more potent. The leukocyte infiltrate almost exclusively neutrophilic nature. A human antagonist, growth-related oncogene (GRO)-α(8–73), inhibited calcium mobilization induced by MIP-2, but not...
Emerging data suggest that polymorphonuclear leukocytes (PMNLs) can play an important role in Ag-dependent immune responses. Therefore, we have assessed the involvement of these cells development organ-specific autoimmune disease, experimental encephalomyelitis (EAE), mouse. Depletion peripheral blood PMNLs beginning day 8 after immunization significantly delayed and some cases totally prevented clinical EAE mice. 1 before sensitization continuing until 7 postimmunization had no effect on...
Chemokines play an important role in atherogenesis and ischemic injury repair; however, prospective data on individual chemokines unstable angina pectoris (UAP) are scarce. Therefore, we assessed chemokine patterns a cohort of patients with UAP.Plasma samples 54 Braunwald class IIIB UAP were examined at baseline for 11 5 inflammatory mediators via multiplex analysis. Levels CC ligand (CCL)-5 (also known as RANTES [regulated activation, normally T-cell expressed, secreted]; 32.7 versus 23.1...
Human neutrophils at inflammatory sites may be an important source of the chemotactic cytokines macrophage protein 1 alpha (M1P-1 alpha; a C-C chemokine) and interleukin 8 (IL-8; C-X-C chemokine). In this study, we show that microcrystals monosodium urate monohydrate (MSU) calcium pyrophosphate dihydrate (CPPD), major mediators gout pseudogout, differentially regulate production these two chemokines by human neutrophils. Both MSU CPPD increased secretion IL-8 in dose- time-dependent manner,...
Chemokines are essential for homeostasis and activation of the immune system. The chemokine ligand/receptor pairing CCL20/CCR6 is interesting because these molecules display characteristics both homeostatic functions. These dual suggest a role CCR6 in priming effector phases response. However, while has been implicated phase several models, less clear. Herein we analyze two important arms response during experimental autoimmune encephalomyelitis (EAE). Both its ligand CCL20 were up-regulated...