A5085429204- Lymphatic System and Diseases
- Hippo pathway signaling and YAP/TAZ
- Angiogenesis and VEGF in Cancer
- Genetic and Kidney Cyst Diseases
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Wnt/β-catenin signaling in development and cancer
- Planarian Biology and Electrostimulation
- Axon Guidance and Neuronal Signaling
- Renal and related cancers
- Congenital heart defects research
- Inflammasome and immune disorders
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Vascular Malformations and Hemangiomas
- Childhood Cancer Survivors' Quality of Life
- Sympathectomy and Hyperhidrosis Treatments
- Biomarkers in Disease Mechanisms
Uppsala University
2020-2024
The University of Queensland
2013-2023
QIMR Berghofer Medical Research Institute
2007
Lymphatic vessels arise during development through sprouting of precursor cells from veins, which is regulated by known signaling and transcriptional mechanisms.The ongoing elaboration to form a network less well understood.This involves cell polarization, coordinated migration, adhesion, mixing, regression, shape rearrangements.We identified zebrafish mutant, lymphatic cardiac defects 1 (lyc1), with reduced vessel development.A mutation in polycystic kidney disease 1a was responsible for...
ABSTRACT Despite the essential role of lymphatic vasculature in tissue homeostasis and disease, knowledge organ-specific origins endothelial progenitor cells remains limited. The assumption that most murine embryonic (LECs) are venous derived has recently been challenged. Here, we show dermal blood capillary plexus constitutes an additional, local source LECs contributes to formation vascular network. We describe a novel mechanism whereby rare PROX1-positive exit Ccbe1-dependent manner...
Abstract Despite a growing catalog of secreted factors critical for lymphatic network assembly, little is known about the mechanisms that modulate expression level these molecular cues in blood vascular endothelial cells (BECs). Here, we show BEC‐specific transcription factor, SOX7, plays crucial role non‐cell‐autonomous manner by modulating angiocrine signals to pattern vessels. While SOX7 not expressed (LECs), conditional loss function mouse embryos causes dysmorphic dermal phenotype. We...
ABSTRACT During embryonic development, lymphatic endothelial cell (LEC) precursors are distinguished from blood cells by the expression of Prospero-related homeobox 1 (Prox1), which is essential for vasculature formation in mouse and zebrafish. Prox1 initiation precedes LEC sprouting migration, serving as marker specified LECs. Despite its crucial role upstream regulation LECs remains to be uncovered. SOX18 COUP-TFII thought regulate mice binding promoter region. However, specific studied...
The migration of lymphatic endothelial cells (LECs) is key for the development complex and vast vascular network that pervades most tissues in an organism. In zebrafish, arterial intersegmental vessels together with chemokines have been shown to promote cell from horizontal myoseptum (HM). We observed emergence mural around arteries coincides departure HM which raised possibility LEC migration. Our live imaging ablation experiments revealed LECs migrate slower fail establish absence cells....
Abstract Background Lymphatic vessels play key roles in tissue fluid homeostasis, immune cell trafficking and diverse disease settings. Lymphangiogenesis requires lymphatic endothelial (LEC) differentiation, proliferation, migration, co‐ordinated network formation, yet the transcriptional regulators underpinning these processes remain to be fully understood. The transcription factor MAFB was recently identified as essential for lymphangiogenesis zebrafish cultured human LECs. is activated...
Previous studies have shown that Vasohibin 1 (Vash1) is stimulated by VEGFs in endothelial cells and its overexpression interferes with angiogenesis
Lymphatic vascular development is regulated by well-characterized signaling and transcriptional pathways. These pathways regulate lymphatic endothelial cell (LEC) migration, motility, polarity, morphogenesis. Canonical non-canonical WNT are known to control LEC polarity of vessels valves. PKD1, encoding Polycystin-1, the most commonly mutated gene in polycystic kidney disease but has also been shown be essential The mechanism which Pkd1 acts during lymphangiogenesis remains unclear.Here we...
Abstract Lymphatic vascular development is regulated by well-characterised signalling and transcriptional pathways. These pathways regulate lymphatic endothelial cell (LEC) migration, motility, polarity morphogenesis. Canonical non-canonical WNT are known to control LEC of vessels valves. PKD1 , encoding Polycystin-1, the most commonly mutated gene in polycystic kidney disease but has also been shown be essential The mechanism which Pkd1 acts during lymphangiogenesis remains unclear. Here we...
<h3>Background</h3> Quantitative and qualitative changes affecting the lymphatic network are associated with limb joint diseases including rheumatoid arthritis osteoarthritis at adult age. However how lymphatics develop during embryogenesis in limbs remain largely unexplored. <h3>Objectives</h3> To describe for first time lymphangiogenesis mouse forelimbs joints early embryogenesis. <h3>Methods</h3> Prox1 is a key transcription factor involved specification remodelling. Taking advantage of...
Abstract Lymphatic vessels play a role in several physiological and pathological processes including tissue fluid homeostasis, dietary fat absorption, immunosurveillance, immunomodulation. During embryonic development, lymphatic endothelial cell (LEC) precursors are distinguished from blood cells by the expression of transcription factor Prospero-related homeobox 1 ( PROX1). PROX1 is essential for vascular network formation mouse zebrafish. The initiation precedes LEC sprouting migration,...
Lymphangiogenesis is the formation of lymphatic vessels from pre-existing vessels, a dynamic process that requires cell migration. Regardless location, endothelial (LEC) progenitors probe their surroundings while migrating to form network. Lymphatic development regulation depends on transcription factor MAFB in different species. Zebrafish Mafba, expressed LEC progenitors, essential for migration trunk. However, transcriptional mechanism orchestrate beds remains elusive. Here, we uncover...
ABSTRACT The migration of lymphatic endothelial cells (LECs) is key for the development complex and vast vascular network that pervades most tissues in an organism. In zebrafish, arterial intersegmental vessels together with chemokines have been shown to promote cell from horizontal myoseptum (HM). Here we found LECs departure HM coincides emergence mural around arteries, raising possibility LEC migration. Our live imaging ablation experiments revealed migrate slower fail establish absence...
Abstract Despite a growing catalogue of secreted factors critical for lymphatic network assembly, little is known about the mechanisms that modulate expression level these molecular cues in blood vascular endothelial cells (BECs). Here, we show BEC-specific transcription factor, SOX7, plays crucial role vessel patterning by modulating lymphangiocrine signals. While SOX7 not expressed (LECs), loss function mouse embryos causes dysmorphic dermal phenotype. We identify novel distant regulatory...