A5078195727- Angiogenesis and VEGF in Cancer
- Hippo pathway signaling and YAP/TAZ
- Axon Guidance and Neuronal Signaling
- Zebrafish Biomedical Research Applications
- Cell Adhesion Molecules Research
- Congenital heart defects research
- Kruppel-like factors research
- Developmental Biology and Gene Regulation
- Barrier Structure and Function Studies
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Meat and Animal Product Quality
- Lymphatic System and Diseases
- Tracheal and airway disorders
- Mathematical Biology Tumor Growth
- Autophagy in Disease and Therapy
- 14-3-3 protein interactions
- Single-cell and spatial transcriptomics
- HER2/EGFR in Cancer Research
- Caveolin-1 and cellular processes
- Fiscal Policy and Economic Growth
- Glycosylation and Glycoproteins Research
- Economic Policies and Impacts
German Centre for Cardiovascular Research
2016-2025
Max Delbrück Center
2016-2025
Charité - Universitätsmedizin Berlin
1991-2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2016-2024
Einstein Center for Neurosciences Berlin
2023-2024
University of North Carolina at Chapel Hill
2024
University of Hong Kong
2024
KU Leuven
2013-2022
VIB-KU Leuven Center for Cancer Biology
2017-2022
Cancer Research UK
2010-2020
Vascular endothelial growth factor (VEGF-A) is a major regulator of blood vessel formation and function. It controls several processes in cells, such as proliferation, survival, migration, but it not known how these are coordinately regulated to result more complex morphogenetic events, tubular sprouting, fusion, network formation. We show here that VEGF-A angiogenic sprouting the early postnatal retina by guiding filopodial extension from specialized cells situated at tips vascular sprouts....
The association of pericytes (PCs) to newly formed blood vessels has been suggested regulate endothelial cell (EC) proliferation, survival, migration, differentiation, and vascular branching. Here, we addressed these issues using PDGF-B– PDGF receptor-β (PDGFR-β)–deficient mice as in vivo models brain angiogenesis the absence PCs. Quantitative morphological analysis showed that mutants have normal microvessel density, length, number branch points. However, PCs correlates with hyperplasia,...
Branching morphogenesis in the mammalian lung and Drosophila trachea relies on precise localization of secreted modulators epithelial growth to select branch sites direct elongation, but intercellular signals that control blood vessel branching have not been previously identified. We found VEGF 120/120 mouse embryos, engineered express solely an isoform VEGF-A lacks heparin-binding, therefore extracellular matrix interaction domains, exhibited a specific decrease capillary formation. This...
The blood–brain barrier (BBB) is confined to the endothelium of brain capillaries and indispensable for fluid homeostasis neuronal function. In this study, we show that endothelial Wnt/β-catenin (β-cat) signaling regulates induction maintenance BBB characteristics during embryonic postnatal development. Endothelial specific stabilization β-cat in vivo enhances maturation, whereas inactivation causes significant down-regulation claudin3 (Cldn3), up-regulation plamalemma vesicle-associated...
Several platelet-derived growth factor (PDGF) and vascular endothelial (VEGF) family members display C-terminal protein motifs that confer retention of the secreted factors within pericellular space. To address role PDGF-B in vivo, we deleted motif by gene targeting mice. This resulted defective investment pericytes microvessel wall delayed formation renal glomerulus mesangium. Long-term effects lack included severe retinal deterioration, glomerulosclerosis, proteinuria. We conclude...
Sprouting angiogenesis is a dynamic process in which endothelial cells collectively migrate, shape new lumenized tubes, make connections, and remodel the nascent network into hierarchically branched functionally perfused vascular bed. Endothelial sprout adopt two distinct cellular phenotypes--known as tip stalk cells--with specialized functions gene expression patterns. VEGF Notch signaling engage an intricate cross talk to balance cell formation regulate directed migration proliferation. In...
Patterning of functional blood vessel networks is achieved by pruning superfluous connections. The cellular and molecular principles regression are poorly understood. Here we show that mediated dynamic polarized migration endothelial cells, representing anastomosis in reverse. Establishing analyzing the first axial polarity map all cells a remodeling vascular network, propose balanced movement maintains primitive plexus under low shear conditions metastable state. We predict flow-induced...