A5020305163- Angiogenesis and VEGF in Cancer
- Chronic Lymphocytic Leukemia Research
- Renal and related cancers
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Kruppel-like factors research
- Immunotherapy and Immune Responses
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Axon Guidance and Neuronal Signaling
- Immune cells in cancer
- Hippo pathway signaling and YAP/TAZ
- Neonatal Respiratory Health Research
- Protein Tyrosine Phosphatases
- TGF-β signaling in diseases
- Acute Lymphoblastic Leukemia research
- RNA modifications and cancer
- Lymphoma Diagnosis and Treatment
- Protease and Inhibitor Mechanisms
- Lymphatic System and Diseases
- Barrier Structure and Function Studies
- Immunodeficiency and Autoimmune Disorders
- Pregnancy and preeclampsia studies
University of Gothenburg
1996-2025
Uppsala University
2015-2024
Science for Life Laboratory
2014-2020
BioInvent (Sweden)
2010
Karolinska Institutet
1999-2009
Sahlgrenska University Hospital
2001-2008
Matrix Research (United States)
2006-2007
Fred Hutch Cancer Center
2000-2001
Ludwig Cancer Research
1999-2001
University of Washington
2001
ABSTRACT Development of a vascular system involves the assembly two principal cell types – endothelial cells and smooth muscle cells/pericytes (vSMC/PC) into many different blood vessels. Most, if not all, vessels begin as tubes that subsequently acquire vSMC/PC coating. We have previously shown PDGF-B is critically involved in recruitment pericytes to brain capillaries kidney glomerular capillary tuft. Here, we used desmin α-smooth actin (ASMA) markers analyze development PDGF-B−/−...
The association of pericytes (PCs) to newly formed blood vessels has been suggested regulate endothelial cell (EC) proliferation, survival, migration, differentiation, and vascular branching. Here, we addressed these issues using PDGF-B– PDGF receptor-β (PDGFR-β)–deficient mice as in vivo models brain angiogenesis the absence PCs. Quantitative morphological analysis showed that mutants have normal microvessel density, length, number branch points. However, PCs correlates with hyperplasia,...
Several platelet-derived growth factor (PDGF) and vascular endothelial (VEGF) family members display C-terminal protein motifs that confer retention of the secreted factors within pericellular space. To address role PDGF-B in vivo, we deleted motif by gene targeting mice. This resulted defective investment pericytes microvessel wall delayed formation renal glomerulus mesangium. Long-term effects lack included severe retinal deterioration, glomerulosclerosis, proteinuria. We conclude...
ABSTRACT PDGF-A−/− mice lack lung alveolar smooth muscle cells (SMC), exhibit reduced deposition of elastin fibres in the parenchyma, and develop emphysema due to complete failure alveogenesis. We have mapped expression PDGF-A, PDGF receptor-α, tropoelastin, α-actin desmin developing lungs from wild type pre- postnatal ages order get insight into mechanisms PDGF-A-induced SMC formation deposition. PDGF-A was expressed by epithelium. Clusters PDGF-Rα-positive (PDGF-Rα+) mesenchymal occurred...
SummaryAngiogenesis, the process by which new blood vessels arise from preexisting ones, is critical for embryonic development and an integral part of many disease processes. Recent studies have provided detailed information on how angiogenic sprouts initiate, elongate, branch, but less known about these processes cease. Here, we show that S1PR1, a receptor blood-borne bioactive lipid sphingosine-1-phosphate (S1P), inhibition angiogenesis acquisition vascular stability. Loss S1PR1 leads to...
ABSTRACT Kidney glomerulus mesangial cells fail to develop in mice carrying targeted null mutations the platelet-derived growth factor (PDGF)-B or PDGF-Rβ genes. We have examined pattern of expression these genes and smooth muscle markers during kidney development, address possible mechanisms underlying mutant phenotypes. In wild-type embryos, PDGF-B was expressed vascular endothelial cells, particularly capillary developing glomeruli, whereas found perivascular mesenchymal renal cortex....
ABSTRACT Reciprocal defects in signaling between the myotome and sclerotome compartments of somites PDGFRαand Myf5 mutant embryos lead to alterations formation vertebrae ribs. To investigate significance these observations, we have examined role PDGF developing somite. PDGFA ligand expression was not detected null promoter activity regulated by vitro. stimulated chondrogenesis somite micromass cultures as well when knocked into locus, resulting increased vertebral rib development. fully...
Normal blood microvessels are lined by pericytes, which contribute to microvessel development and stability through mechanisms that poorly understood. Pericyte deficiency has been implicated in the pathogenesis of microvascular abnormalities associated with diabetes tumors. However, unambiguous identification pericytes is still a problem because cellular heterogeneity few available molecular markers. Here we describe an approach identify pericyte markers based on transcription profiling...
Given the need for robust and cost-efficient in vitro models to study angiogenesis reproducibly analyze potential pro- antiangiogenic compounds preclinical studies, we developed a 3-dimensional assay that is based on collagen gel-embedded, size-defined spheroids generated from cultured human umbilical vein endothelial cells (HUVECs). Despite its wide distribution, limitations, sensitivity, robustness, improvements, capacity of this functional screening purposes has not been elucidated thus...
NF-κB is constitutively activated in chronic lymphocytic leukemia (CLL); however, the implicated molecular mechanisms remain largely unknown. Thus, we performed targeted deep sequencing of 18 core complex genes within pathway a discovery and validation CLL cohort totaling 315 cases. The most frequently mutated gene was NFKBIE (21/315 cases; 7%), which encodes IκBε, negative regulator normal B cells. Strikingly, 13 these cases carried an identical 4-bp frameshift deletion, resulting truncated...
Glioblastoma (GBM) is refractory to immune checkpoint inhibitor (ICI) therapy. We sought determine what extent this evasion due intrinsic properties of the tumor cells versus specialized context brain, and if it can be reversed.
Pathological angiogenesis is an integral component of many diseases. Antiangiogenesis and vascular targeting are therefore promising new therapeutic principles. However, few endothelial-specific putative drug targets have been identified, information still limited about molecular processes. Here we aimed at determining the endothelial cell-specific core transcriptome in vivo.Analysis publicly available microarray data identified a mixed vascular/lung cluster 132 genes that correlated with...
We and others have recently shown that tumor characteristics are altered throughout progression. These findings emphasize the need for re-examination of at relapse led to recommendations from ESMO Swedish Breast Cancer group. Here, we aim determine whether molecular subtypes in breast cancer metastases confer clinically relevant prognostic information patients.
The importance of perivascular–endothelial cell interactions during blood vessel development is discussed in the light recent findings platelet‐derived growth factor‐B, factor receptor‐β, angiopoietin‐1 and tie‐2 knockout mice, which all show deficient perivascular cells. initial formation networks endothelial tubes (vasculogenesis) does not seem to depend on cells but subsequent remodeling relies mesenchymal–endothelial short‐range signaling. Based receptor a general model for role factors...
Abstract Purpose Fertility preservation for pre-pubertal girls undergoing gonadotoxic cancer treatments and women with systemic cancers at high risk ovarian metastasis remains limited. Current options, such as cortex transplantation, reintroducing malignant cells. This study presents a novel approach focusing on ex vivo folliculogenesis mature oocyte retrieval cryopreservation, mitigating this risk. Methods experimental optimized an perfusion system in sheep, refining gonadotropin...