A5078058223- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- RNA regulation and disease
- Skin and Cellular Biology Research
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Barrier Structure and Function Studies
- RNA Research and Splicing
- Alzheimer's disease research and treatments
- Neurological diseases and metabolism
- Neonatal and fetal brain pathology
- Cellular Mechanics and Interactions
- Traumatic Brain Injury and Neurovascular Disturbances
- Stroke Rehabilitation and Recovery
- S100 Proteins and Annexins
- Anesthesia and Neurotoxicity Research
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Glioma Diagnosis and Treatment
- Complement system in diseases
- RNA Interference and Gene Delivery
- Prion Diseases and Protein Misfolding
- Retinal Development and Disorders
- Epigenetics and DNA Methylation
University of Gothenburg
2015-2024
Florey Institute of Neuroscience and Mental Health
2014-2024
University of Newcastle Australia
2015-2024
Hunter Medical Research Institute
2015-2017
Sahlgrenska University Hospital
1994-2017
Czech Academy of Sciences
2010
Florida State University
2007
National High Magnetic Field Laboratory
2007
University of Iowa
2006
Durham University
2003
P Levéen, M Pekny, S Gebre-Medhin, B Swolin, E Larsson, and C Betsholtz Department of Medical Biochemistry, University Göteborg, Sweden.
Reactive astrocytes in neurotrauma, stroke, or neurodegeneration are thought to undergo cellular hypertrophy, based on their morphological appearance revealed by immunohistochemical detection of glial fibrillary acidic protein, vimentin, nestin, all them forming intermediate filaments, a part the cytoskeleton. Here, we used recently established dye-filling method reveal full three-dimensional shape assessing morphology reactive two neurotrauma models. Both denervated hippocampal region and...
Reactive astrocytes are thought to protect the penumbra during brain ischemia, but direct evidence has been lacking due absence of suitable experimental models. Previously, we generated mice deficient in two intermediate filament (IF) proteins, glial fibrillary acidic protein (GFAP) and vimentin, whose upregulation is hallmark reactive astrocytes. GFAP −/− Vim exhibit attenuated posttraumatic gliosis, improved integration neural grafts, regeneration. Seven days after middle cerebral artery...
The regenerative capacity of the CNS is extremely limited. reason for this unclear, but glial cell involvement has been suspected, and oligodendrocytes have implicated as inhibitors neuroregeneration (Chen et al., 2000, GrandPre 2000; Fournier 2001). role astrocytes in process was proposed remains incompletely understood (Silver Miller, 2004). Astrocyte activation (reactive gliosis) accompanies neurotrauma, stroke, neurodegenerative diseases, or tumors. Two prominent hallmarks reactive...
The accumulation of aggregated amyloid-β (Aβ) in amyloid plaques is a neuropathological hallmark Alzheimer's disease (AD). Reactive astrocytes are intimately associated with plaques; however, their role AD pathogenesis unclear. We deleted the genes encoding two intermediate filament proteins required for astrocyte activation—glial fibrillary acid protein (Gfap) and vimentin (Vim)—in transgenic mice expressing mutant human precursor presenilin-1 (APP/PS1). gene deletions increased plaque...
In response to injury of the central nervous system, astrocytes become reactive and express high levels intermediate filament (IF) proteins glial fibrillary acidic protein (GFAP), vimentin, nestin. We have shown that in mice deficient for both GFAP vimentin (GFAP−/−vim−/−) cannot form IFs even when nestin is expressed are thus devoid their state. Here, we studied reaction system GFAP−/−, vimentin−/−, or GFAP−/−vim−/− mice. Glial scar formation appeared normal after spinal cord brain lesions...
ABSTRACT Kidney glomerulus mesangial cells fail to develop in mice carrying targeted null mutations the platelet-derived growth factor (PDGF)-B or PDGF-Rβ genes. We have examined pattern of expression these genes and smooth muscle markers during kidney development, address possible mechanisms underlying mutant phenotypes. In wild-type embryos, PDGF-B was expressed vascular endothelial cells, particularly capillary developing glomeruli, whereas found perivascular mesenchymal renal cortex....
Intermediate filaments are general constituents of the cytoskeleton. The function these structures and requirement for different types intermediate filament proteins by individual cells only partly understood. Here we have addressed role specific protein partnerships in formation astrocytes. Astrocytes may express three proteins: glial fibrillary acidic (GFAP), vimentin, nestin. We used mice with targeted mutations GFAP or vimentin genes, both, to study impact loss either both on cultured...
Hepatic stellate cells are considered to be liver-specific pericytes that play a key role in liver fibrosis. Because these express desmin and smooth muscle α-actin, they were assumed of myogenic origin. This hypothesis became doubtful when it was reported also glial fibrillary acidic protein neural cell adhesion molecule. In the present study, we show activated nestin, class VI intermediate filament originally identified as marker for stem cells. Expression nestin first studied during...