A5021183543- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Axon Guidance and Neuronal Signaling
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Fibroblast Growth Factor Research
- Epigenetics and DNA Methylation
- Developmental Biology and Gene Regulation
- Renal and related cancers
- Cleft Lip and Palate Research
- Neurogenesis and neuroplasticity mechanisms
- Hippo pathway signaling and YAP/TAZ
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Angiogenesis and VEGF in Cancer
- RNA and protein synthesis mechanisms
- Bone Metabolism and Diseases
- Wnt/β-catenin signaling in development and cancer
- Craniofacial Disorders and Treatments
- Virus-based gene therapy research
- Bone health and treatments
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
Icahn School of Medicine at Mount Sinai
2015-2024
Hospital General Universitario De Valencia
2021
Tisch Cancer Institute
2016-2018
Tisch Hospital
2016-2018
Stemcell Technologies
2018
Fred Hutch Cancer Center
2001-2015
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
1999-2008
North Seattle College
1997-2006
Case Western Reserve University
2001
Cancer Research Center
2001
ABSTRACT Neural crest cells are multipotential stem that contribute extensively to vertebrate development and give rise various cell tissue types. Determination of the fate mammalian neural has been inhibited by lack appropriate markers. Here, we make use a two-component genetic system for indelibly marking progeny cranial during tooth mandible development. In first mouse line, Cre recombinase is expressed under control Wnt1 promoter as transgene. Significantly, transgene expression limited...
A general strategy for selecting insertion mutations in mice has been devised. Constructs lacking a promoter and including beta-galactosidase gene, or reporter gene encoding protein with both neomycin phosphotransferase activity, were designed so that activation of the depends on its within an active transcription unit. Such events create mutation tagged allow expression to be followed by activity. Introduction trap constructs into embryonic stem (ES) cells electroporation retroviral...
Mice with mutations in four nonreceptor tyrosine kinase genes, fyn , src yes and abl were used to study the role of these kinases long-term potentiation (LTP) relation LTP spatial learning memory. All expressed hippocampus. Mutations did not interfere either induction or maintenance LTP. However, mutants, was blunted even though synaptic transmission two short-term forms plasticity, paired-pulse facilitation post-tetanic potentiation, normal. In parallel blunting LTP, mutants showed impaired...
ABSTRACT A subpopulation of neural crest termed the cardiac is required in avian embryos to initiate reorganization outflow tract developing cardiovascular system. In mammalian embryos, it has not been previously experimentally possible study long-term fate this population, although there strong inference that a similar population exists and perturbed number genetic teratogenic contexts. We have employed two-component system based on Cre/lox recombination label indelibly entire mouse at time...
Platelet-derived growth factor, a major mitogen and chemoattractant for number of cell types, is implicated in the processes wound healing, tumorigenesis, differentiation recognized by two receptors, alpha beta. To begin understanding role these receptors development, beta-receptor-deficient mice were generated gene targeting ES cells. Mutant are hemorrhagic, thrombocytopenic, severely anemic, exhibit defect kidney glomeruli because lack mesangial cells, die at or shortly before birth....
The ROSAβgeo26 (ROSA26) mouse strain was produced by random retroviral gene trapping in embryonic stem cells. Staining of ROSA26 tissues and fluorescence-activated cell sorter-Gal analysis hematopoietic cells demonstrates ubiquitous expression the proviral βgeo reporter gene, bone marrow transfer experiments illustrate general utility this for chimera transplantation studies. trap vector has integrated into a region that produces three transcripts. Two transcripts, lost homozygous animals,...
ABSTRACT Platelet-derived growth factors (PDGFs) have been implicated in the control of cell proliferation, survival and migration. Patch mutant mice harbor a deletion including PDGFα receptor gene exhibit defects neural crest origin which affect pigmentation heterozygotes cranial bones homozygotes. To verify role PDGFαR during development, carrying targeted null mutation were generated. No phenotype was observed heterozygotes. Homozygotes die embryonic development incomplete cephalic...
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and publicly available genome-wide collection mouse knockouts would be significantly enabling for biomedical discovery. To date, published exist only about 10% genes. Furthermore, many these are limited utility because they have not been made or phenotyped standardized ways, freely to researchers. It is time harness new technologies efficiencies production mount high-throughput international effort...
As conditional genetic strategies advance, the need for multiple site-specific recombinase systems has emerged. To meet this in part, we have targeted constitutive ROSA26 locus to create a mouse strain with generalized expression of enhanced version FLP (FLPe). This is designated FLPeR ("flipper"). Using strain, extensive target gene recombination can be achieved most tissue types, including cells developing germ line. mice therefore serve two important functions: as source many different...
Targeted disruption of the c-src proto-oncogene in mice has shown that src expression is required for normal bone resorption, since src-deficient mutants develop osteopetrosis. To evaluate mechanisms by which src-deficiency affects osteoclast function, we treated with stimulants IL-1, parathyroid hormone, and hormone-related protein, analyzed effects quantitative histomorphometry electron microscopy. Increased numbers multinucleated cells morphological characteristics osteoclasts appeared on...
Receptor tyrosine kinases (RTKs) direct diverse cellular and developmental responses by stimulating a relatively small number of overlapping signaling pathways. Specificity may be determined RTK expression patterns or differential activation individual To address this issue we generated knock-in mice in which the extracellular domain mouse platelet-derived growth factor alpha receptor (PDGFalphaR) is fused to cytosolic Drosophila Torso (alpha(Tor)) fibroblast 1 (alpha(FR)). alpha(Tor)...
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array genomic engineering possibilities, efficient recombination mammalian cells has only been observed with Cre recombinase. Here we report de novo synthesis mouse codon-optimized FLP (FLPo) ΦC31 (ΦC31o) SSRs, which result efficiencies similar to Cre.