A5058213173- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Lymphatic System and Diseases
- Cell Adhesion Molecules Research
- Axon Guidance and Neuronal Signaling
- TGF-β signaling in diseases
- Kruppel-like factors research
- PI3K/AKT/mTOR signaling in cancer
- Epigenetics and DNA Methylation
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Zebrafish Biomedical Research Applications
- Pregnancy and preeclampsia studies
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Retinal Diseases and Treatments
- Mesenchymal stem cell research
- Protein Kinase Regulation and GTPase Signaling
- Liver physiology and pathology
- Cancer Cells and Metastasis
- Proteoglycans and glycosaminoglycans research
- Congenital heart defects research
- Chronic Myeloid Leukemia Treatments
- Reproductive System and Pregnancy
Jobu University
2014-2023
Czech Academy of Sciences, Institute of Physiology
2013-2021
Tokyo University of Science
2003-2015
Tokyo Medical and Dental University
2007-2015
The University of Tokyo
2003-2014
Tokyo Metropolitan Komagome Hospital
2005-2013
Molecular Oncology (United States)
2008-2013
Cancer Genetics (United States)
2013
Roswell Park Comprehensive Cancer Center
2013
Breast Center
2011
Tyrosine-specific protein kinase activity of the epidermal growth factor (EGF) receptor, pp60"mc and ppllOgw"w was inhibited in vitro by an isoflavone genistein.The inhibition competitive with respect to ATP noncompetitive a phosphate acceptor, histone H2B.By contrast, genistein scarcely enzyme activities serine-and threonine-specific kinases such as CAMP-dependent kinase, phosphorylase Ca2+/phospholipiddependent C. When effect on phosphorylation EGF receptor examined cultured A43 1 cells,...
Vascular endothelial growth factor (VEGF) is a homodimeric peptide which binds to two structurally related tyrosine kinase receptors denoted Flt1 and KDR. In order compare the signal transduction via these receptors, human KDR proteins were stably expressed in porcine aortic cells. Binding analyses using 125I-VEGF revealed Kd values of 16 pM for 760 Cultured umbilical vein (HUVE) cells found express distinct populations binding sites with affinities similar those KDR, respectively. The...
The vascular endothelial growth factor (VEGF) family has recently expanded by the identification and cloning of three additional members, namely VEGF-B, VEGF-C, VEGF-D. In this study we demonstrate that VEGF-B binds selectively to VEGF receptor-1/Flt-1. This binding can be blocked excess VEGF, indicating interaction sites on receptor are at least partially overlapping. Mutating putative receptor-1/Flt-1 determinants Asp 63 , 64 Glu 67 alanine residues in reduced affinity receptor-1 but did...
Vascular endothelial growth factor (VEGF) mediates cell proliferation, angiogenesis, and vascular permeability via the receptors, KDR/Flk-1 Flt-1. Recently, a gene encoding polypeptide with about 25% amino acid identity to mammalian VEGF was identified in genome of Orf virus (OV), parapoxvirus that affects sheep goats occasionally, humans, generate lesions angiogenesis. In this study, we examined biological activities receptor OV-derived NZ-7 (VEGF-E). VEGF-E found be dimer 20 kDa no basic...
Antiangiogenic therapy for the treatment of cancer and other neovascular diseases is desired to be selective pathological angiogenesis lymphangiogenesis. Macrophage colony-stimulating factor (M-CSF), a cytokine required differentiation monocyte lineage cells, promotes formation high-density vessel networks in tumors therefore possesses therapeutic potential as an M-CSF inhibitor. However, physiological role vascular lymphatic development, well precise mechanisms underlying antiangiogenic...
Placenta growth factor (PlGF) belongs to the family of vascular endothelial factors (VEGFs). It binds flt-1 VEGF receptor but not KDR/flk-1 which is thought mediate most angiogenic and proliferative effects VEGF. Three PlGF isoforms are produced by alternative splicing. PlGF-1 PlGF-3 differ from PlGF-2 since they lack exon 6 encoded peptide bestows upon its heparin binding properties. Cross-linking experiments revealed that 125I-PlGF-2 two cell surface receptors in a dependent fashion. The...
Four vascular endothelial growth factor (VEGF) splice variants containing 121, 165, 189, and 206 amino acids are produced from a single human gene as result of alternative splicing. VEGF121 is not heparin-binding protein, while the other VEGF species possess heparin binding ability. YU-ZAZ6 melanoma cells expressed mRNA encoding receptor flt-1, but KDR/flk-1. Both VEGF165 bound to receptors these cells. Unexpectedly, inhibited well Digestion with heparinase also both variants. The ability...
Vascular Endothelial Growth Factor (VEGF) has been typically considered to be an endothelial-specific growth factor. However, it was recently demonstrated that VEGF can interact with non endothelial cells. In this study, we tested whether vascular smooth muscles cells (VSMCs) express receptors, such as flk-1, flt-1, and neuropilin (NP)-1, respond in vitro. cultured VSMCs, flk-1 flt-1 expression inversely related cell density. The of down-regulated increasing passage numbers. NP-1 levels were...
Adipose tissue is a structure highly specialized in energy storage. The adipocyte the parenchymal component of adipose and known to be mesoderm or neuroectoderm origin; however, development remains poorly understood. Here, we investigated by analyzing postnatal epididymal (EAT) mouse. EAT was found generated from non-adipose during first 14 days. From day 1 (P1) P4, composed multipotent progenitor cells that lack adipogenic differentiation capacity vitro, can regarded as being 'undetermined'...
Lymphatic vessel growth, or lymphangiogenesis, is regulated by vascular endothelial growth factor-C (VEGF-C) and -D via VEGF receptor 3 (VEGFR-3). Recent studies suggest that VEGF, which does not bind to VEGFR-3, can also induce lymphangiogenesis through unknown mechanisms. To dissect the pathway triggers VEGFR-3–independent we used both transgenic adenoviral overexpression of placenta factor (PlGF) VEGF-E, are specific activators VEGFR-1 -2, respectively. Unlike PlGF, VEGF-E induced...