A5100770850- Developmental Biology and Gene Regulation
- Angiogenesis and VEGF in Cancer
- Congenital heart defects research
- TGF-β signaling in diseases
- Reproductive System and Pregnancy
- Lymphatic System and Diseases
- Epigenetics and DNA Methylation
- Endometriosis Research and Treatment
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Pregnancy-related medical research
- Mitochondrial Function and Pathology
- Ear Surgery and Otitis Media
- Genetic and Kidney Cyst Diseases
- Uterine Myomas and Treatments
- Genetic Syndromes and Imprinting
- Wnt/β-catenin signaling in development and cancer
- Ear and Head Tumors
- Metabolism and Genetic Disorders
- Protease and Inhibitor Mechanisms
- Renal and related cancers
- Fibroblast Growth Factor Research
- Nasal Surgery and Airway Studies
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
Hallym University
2016-2024
All India Institute of Medical Sciences Bhopal
2018-2024
Ewha Womans University
2024
Konyang University
2024
All India Institute of Medical Sciences
2018-2024
All India Institute of Medical Sciences Raipur
2018-2023
National Institute of Mental Health and Neurosciences
2023
All India Institute of Medical Sciences Rishikesh
2023
Central Drug Research Institute
1995-2021
International Centre for Genetic Engineering and Biotechnology
2020
The vascular endothelial growth factor (VEGF) family has recently expanded by the identification and cloning of three additional members, namely VEGF-B, VEGF-C, VEGF-D. In this study we demonstrate that VEGF-B binds selectively to VEGF receptor-1/Flt-1. This binding can be blocked excess VEGF, indicating interaction sites on receptor are at least partially overlapping. Mutating putative receptor-1/Flt-1 determinants Asp 63 , 64 Glu 67 alanine residues in reduced affinity receptor-1 but did...
Vascular endothelial growth factor-C (VEGF-C) is a recently characterized member of the VEGF family angiogenic polypeptides. We demonstrate here that VEGF-C in vitro when added to bovine aortic or lymphatic (BAE and BLE) cells but has little no effect on microvascular (BME) cells. As reported previously for VEGF, basic fibroblast factor (bFGF) induced synergistic response all three lines. Unexpectedly, also synergized assessed BAE Characterization receptor (VEGFR) expression revealed BME,...
Several methods for auricular cartilage engineering use tissue techniques. However, an ideal method has not been reported. To address this issue, we developed a strategy to engineer using silk fibroin (SF) and polyvinyl alcohol (PVA) hydrogel. We constructed different hydrogels with various ratios of SF PVA by salt leaching, silicone mold casting, freeze-thawing methods. characterized each the in terms swelling ratio, tensile strength, pore size, thermal properties, morphologies, chemical...
ABSTRACT VEGF-C is a recently discovered secreted polypeptide related to the angiogenic mitogen VEGF. We have isolated quail cDNA and shown that its protein product from transfected cells interacts with avian VEGFR3 VEGFR2. In situ hybridization shows mRNA strongly expressed in regions destined be rich lymphatic vessels, particularly mesenteries, mesocardium myotome, region surrounding jugular veins, kidney. These expression sites are similar those observed mouse embryo (E. Kukk, A....
Transforming growth factor-beta (TGF-B) plays an important role in embryo implantation; however, TGF-B requires liberation from its inactive latent forms (i.e., large complex [LLC] and small [SLC]) to biologically active monomer or dimer) order act on receptors (TGF-BRs), which turn activate SMAD2/3. Activation of TGF-B1 complexes the uterus is not yet deciphered. We investigated uterine form during implantation, decidualization, delayed implantation. Our study, utilizing nonreducing...
Endometriosis is a prevalent gynecological disorder that eventually gives rise to painful invasive lesions. Increased levels of transforming growth factor-beta 1 (TGF-B1) have been reported in endometriosis. However, details the effects high TGF-B1 on downstream signaling ectopic endometrial tissue remain obscure. We induced endometriotic lesions mice by surgical auto-transplantation tissues peritoneal regions. then treated (ectopic and eutopic tissues) nonendometriotic (only animal groups...
Abstract Integrin beta8 (ITGB8) is involved in the endometrial receptivity. The blastocyst first interacts with luminal epithelial cells during its implantation; therefore, we have investigated signaling of ITGB8 via FAK and VAV-RAC1 cells. was found elevated at late-pre-receptive (day4, 1600 h) receptive (day5, 0500 stages receptivity period mouse. Integrins downstream molecule has demonstrated an increased expression phosphorylation (Y397) endometrium as well isolated post-receptive...
Objectives To reduce the mastoid cavity‐associated problems secondary to canal wall down mastoidectomy, we designed a new surgical procedure that includes reconstruction using free‐floating cartilages and double musculoperiosteal flaps. Study Design Retrospective study. Materials methods Thirty‐three patients were enrolled in this Preoperative postoperative pure tone audiometry/speech discrimination score status (complications EAC status) analyzed. Results Air conduction thresholds...
Integrins (ITGs) are mediators of cell-cell and cell-matrix interactions, which also associated with embryo implantation processes by controlling the interaction blastocyst endometrium. During early pregnancy, ITGbeta8 (ITGB8) has been shown to interact latent transforming growth factor (TGF) beta 1 (TGFB1) at fetomaternal interface. However, precise role ITGB8 in uterus its association not elucidated. Therefore, we attempted ascertain during window process inhibiting function or protein...
In MYCN-amplified neuroblastoma (NB), we noticed that the single compound treatment with HDAC inhibitor vorinostat led to a reprogramming of glycolytic pathway in these cells. This was upregulation fatty acid oxidation (FAO) and oxidative phosphorylation (OXPHOS), allowing cells generate ATP, albeit at reduced rate. behavior dependent on levels MYCN corresponding increase PPARD transcription factors. By integrating metabolic functional studies NB mouse xenografts, demonstrate compensatory...
Tie1 is an endothelial receptor tyrosine kinase essential for development and maintenance of the vascular system. Here we report generation transgenic mice expressing enhanced green fluorescent protein (EGFP) or a chimeric consisting Zeosin resistance marker EGFP under control mouse promoter. Intravital monitoring fluorescence showed that reporter recapitulates expression pattern in developing vasculature, flow cytometry using allowed isolation essentially pure Tie1-expressing cells from...
Polycystic ovarian syndrome (PCOS) is characterized by the presence of multiple follicular cysts, giving rise to infertility due anovulation. This affects about 10% women, worldwide. The exact molecular mechanism leading PCOS remains obscure. RhoGTPase has been associated with oogenesis, but its role in unexplored. Therefore, we attempted elucidate Vav-Rac1 signaling mice model. We generated a model injecting dehydroepiandrosterone (DHEA) for period 20 days. expression levels Rac1, pRac1,...
Objectives/Hypothesis Silk fibroin patching has been used to repair acute tympanic membrane perforations. Here, we describe the advantages and outcomes of this technique for chronic Study Design Individual cohort study. Methods Forty patients were enrolled; half underwent perichondrium myringoplasty, silk patch was applied in remaining patients. We compared closure, otorrhea, complication rates; closure time; postoperative hearing gain; patient satisfaction between two groups. Results...
Abstract The motile cilia of ependymal cells coordinate their beats to facilitate a forceful and directed flow cerebrospinal fluid (CSF). Each cilium originates from basal body with foot protruding one side. A uniform alignment these feet is crucial for the coordination ciliary beating. process by which subdistal appendages body, however, unresolved. Here, we show FGFR1 Oncogene Partner (FOP) useful marker delineating transformation circular, unpolarized appendage into polarized structure...
The Bmp/Smad1 pathway plays a crucial role in developmental processes and tissue homeostasis. Mitogen-activated protein kinase (Mapk)/Erk mediated phosphorylation of Smad1 the linker region leads to degradation, cytoplasmic retention inhibition signaling. While Fgf/Erk has been documented inhibit signaling, several studies also suggests cooperative interaction between these two pathways different context. However, precise molecular this collaborative remain obscure. Here, we identified Xbra...