A5010874845- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Lymphatic System and Diseases
- Biomedical Ethics and Regulation
- Protease and Inhibitor Mechanisms
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Biotechnology and Related Fields
- Pluripotent Stem Cells Research
- Pharmacogenetics and Drug Metabolism
- Cancer, Hypoxia, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Vascular Malformations and Hemangiomas
- Hematopoietic Stem Cell Transplantation
- TGF-β signaling in diseases
- Vascular Tumors and Angiosarcomas
- Ethics in Clinical Research
- Tissue Engineering and Regenerative Medicine
- Neonatal and fetal brain pathology
- CRISPR and Genetic Engineering
- Biomedical and Engineering Education
- Drug Transport and Resistance Mechanisms
- Virus-based gene therapy research
South African Medical Research Council
2016-2025
University of Pretoria
2016-2025
Institut de Biologie Moléculaire et Cellulaire
2013-2023
Mount Sinai Hospital
2022
University of Toronto
2022
KU Leuven
2022
Europe Hospitals
2022
British Columbia Children's Hospital
2022
Hospital for Sick Children
2022
SickKids Foundation
2022
Consumption of a plant-based diet can prevent the development and progression chronic diseases that are associated with extensive neovascularization; however, little is known about mechanisms. To determine whether prevention might be dietary-derived angiogenesis inhibitors, we have fractionated urine healthy human subjects consuming examined fractions for their abilities to inhibit proliferation vascular endothelial cells. Using gas chromatography-mass spectrometry, showed one most potent...
The vascular endothelial growth factor (VEGF) family has recently expanded by the identification and cloning of three additional members, namely VEGF-B, VEGF-C, VEGF-D. In this study we demonstrate that VEGF-B binds selectively to VEGF receptor-1/Flt-1. This binding can be blocked excess VEGF, indicating interaction sites on receptor are at least partially overlapping. Mutating putative receptor-1/Flt-1 determinants Asp 63 , 64 Glu 67 alanine residues in reduced affinity receptor-1 but did...
Tightly controlled proteolytic degradation of the extracellular matrix by invading microvascular endothelial cells is believed to be a necessary component angiogenic process. We have previously demonstrated induction plasminogen activators (PAs) in bovine (BME) three agents that induce angiogenesis vitro: basic FGF (bFGF), PMA, and sodium orthovanadate. Surprisingly, we find these also activator inhibitor-1 (PAI-1) activity mRNA BME cells. transforming growth factor-beta 1 (TGF-beta 1),...
The lymphatic microvasculature is uniquely adapted for the continuous removal of interstitial fluid and proteins an important entry point leukocytes tumor cells. Specialized functions lymphatics suggest differences in molecular composition blood vascular endothelium. However, extent to which two cell types differ still unclear, few molecules that are truly specific endothelial cells have been identified date. We isolated primary microvascular from human skin by immunoselection with marker...
Abstract —Angiopoietin-2 (Ang2) is a ligand for the endothelial cell tyrosine kinase receptor Tie2 and counteracts blood vessel maturation/stability mediated by angiopoietin-1 (Ang1), other known of Tie2. Using degenerate oligonucleotides reverse transcriptase–polymerase chain reaction, we have screened bovine microvascular (BME), aortic, lymphatic, pulmonary artery, transformed fetal aortic cells, as well rat smooth muscle cells Ang1 Ang2 expression. Except high mRNA levels found in BME...
Cellular migration is an essential component of invasive biological processes, many which have been correlated with increase in plasminogen activator production. Endothelial cell occurs vivo during repair vascular lesions and angiogenesis, can be induced vitro by wounding a confluent monolayer cells. By combining the wounded model substrate overlay technique, we show that cells migrating from edges experimental wound display urokinase-type (uPA) activity, this activity reverts to background...
One of the phenotypic hallmarks migrating endothelial cells, both in vivo and vitro, is expression urokinase-type plasminogen activator (u-PA), a key mediator extracellular proteolysis. In study reported here, we have used an vitro model cell migration to explore mechanism this phenomenon. We found that wounding monolayer triggers marked, rapid sustained increase specific high-affinity receptor for u-PA (u-PAr) on surface cells. Migrating cells displayed levels u-PAr mRNAs, was mediated by...
Aims: Angiogenesis is a complex multistep process essential for tumour growth. Vascular endothelial growth factor (VEGF) potent cell mitogen and vascular permeability‐inducing agent. Recent studies have shown that VEGF expression correlated to microvessel density progression. The aim of this study was analyse in series gastrointestinal neuroendocrine tumours. Methods results: Surgical specimens from 28 carcinoids 20 pancreatic endocrine tumours were examined by immunohistochemistry. Intense...
Vascular endothelial growth factor (VEGF) is a potent angiogenic and cell-specific mitogen that stimulates urokinase-type plasminogen activator (uPA) activity in vascular cells. Here, we report VEGF increases the high affinity binding of uPA to same cells this prevented by peptide corresponding receptor (uPAR) factor-like domain uPA. Ligand cross-linking, ligand blotting, uPA-Sepharose chromatography revealed an increase cell surface protein corresponds uPAR on basis its for uPA, Mrof...