A5102977193- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Genetic factors in colorectal cancer
- Genomics and Phylogenetic Studies
- Extracellular vesicles in disease
- Lymphatic System and Diseases
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
- Mesenchymal stem cell research
- Metastasis and carcinoma case studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Caveolin-1 and cellular processes
- Mechanisms of cancer metastasis
- Plant and Fungal Interactions Research
- Electrospun Nanofibers in Biomedical Applications
- Wnt/β-catenin signaling in development and cancer
- Lipid Membrane Structure and Behavior
Korea Advanced Institute of Science and Technology
2010-2025
Seoul National University
1996-2024
Korean Academy of Science and Technology
2023
CHA University
2016-2020
Center for Systems Biology
2020
Massachusetts General Hospital
2020
New Generation University College
1996
Cancer Research Center
1996
Plants have evolved to sophisticated adaptation mechanisms cope with drought stress by reprograming transcriptional networks through responsive transcription factors. NAM, ATAF1-2, and CUC2 (NAC) factors are known be associated various developmental processes tolerance. In this study, we functionally characterized the rice factor OsNAC14. OsNAC14 was predominantly expressed at meiosis stage but is induced drought, high salinity, ABA low temperature in leaves. Overexpression of resulted...
Vascular protection through TIE2 activation is a potential treatment strategy to ameliorate sepsis.
Adipose tissue is a structure highly specialized in energy storage. The adipocyte the parenchymal component of adipose and known to be mesoderm or neuroectoderm origin; however, development remains poorly understood. Here, we investigated by analyzing postnatal epididymal (EAT) mouse. EAT was found generated from non-adipose during first 14 days. From day 1 (P1) P4, composed multipotent progenitor cells that lack adipogenic differentiation capacity vitro, can regarded as being 'undetermined'...
Abstract Tumor-associated lymphatic vessels (LV) serve as a route of cancer dissemination through the prometastatic crosstalk between endothelial cells (LECs) lining LVs and cells. Compared to blood cell-derived angiocrine factors, however, LEC-secreted factors in tumor microenvironment their roles metastasis are poorly understood. Here, we report that ELK3 expressed LECs contributes during growth by providing oncogenic miRNAs exosomes. We found conditioned medium from ELK3-suppressed (LCM)...
Drought is one of the major environmental stresses adversely affecting crop productivity worldwide. Precise characterization genes involved in drought response necessary to develop new varieties with enhanced tolerance. Previously, we identified 66 drought-induced miRNAs rice plants. For further functional investigation miRNAs, applied recombinant codon-optimized Cas9 (rCas9) for single-guide RNAs specifically targeting mature miRNA sequences or sites required biogenesis miRNA. A total 458...
Abstract ZEB1 has intrinsic oncogenic functions that control the epithelial-to-mesenchymal transition (EMT) of cancer cells, impacting tumorigenesis from its earliest stages. By integrating microenvironment signals and being implicated in feedback regulatory loops, appears to be a central switch determines EMT metastasis cells. Here, we found collaborates with ELK3, ternary complex factor belonging ETS family, repress E-cadherin expression. as transcriptional activator ELK3. We first...
Triple-negative breast cancer is a highly aggressive tumor subtype that lacks effective therapeutic targets. Here, we show ELK3 overexpressed in subset of cancers, particular basal-like and normal-like/claudin-low cell lines. Suppression MDA-MB-231 cells led to transdifferentiation from an invasive mesenchymal phenotype non-invasive epithelial both vitro vivo. resulted extensive changes genome expression profiles. Among these, GATA3, master suppressor metastasis, was epigenetically...
Some four-year-olds have never eaten a salad.That's one of the reasons there's Head Start.We give low-income preschool children chance to grow up healthy.And happy.But we need health care volunteers.Especially nutritionists like you.Come show child what strong bodies are made of.Because if you change world child, world.
Abstract Angiopoietin-1 (Ang1), a potential growth factor for therapeutic angiogenesis and vascular stabilization, is known to specifically cluster activate Tie2 in high oligomeric forms, which unique essential process this ligand-receptor interaction. However, highly native Ang1 variants are difficult produce, purify store stable active form. To overcome these limitations, we developed simple dimeric CMP-Ang1 by replacing the N-terminal of with coiled-coil domain cartilage matrix protein...
Sessile plants have evolved distinct mechanisms to respond and adapt adverse environmental conditions through diverse including RNA processing. While the role of processing in stress response is well understood for Arabidopsis thaliana, limited information available rice (Oryza sativa). Here, we show that OsFKBP20-1b, belonging immunophilin family, interacts with splicing factor OsSR45 both nuclear speckles cytoplasmic foci, plays an essential post-transcriptional regulation abiotic...
Summary Whole‐genome annotation error that omits essential protein‐coding genes hinders further research. We developed Target Gene Family Finder ( TGFam‐Finder ), an alternative tool for the structural of containing target domain(s) interest in plant genomes. took considerably reduced run‐time and improved accuracy compared to conventional tools. Large‐scale re‐annotation 50 genomes identified average 150, 166 86 additional far‐red‐impaired response 1, nucleotide‐binding leucine‐rich‐repeat,...
Abstract Recently, new types of base editors, C-to-G editors (CGBEs), that enable cytosine transversions are unachievable with (CBEs) and adenosine (ABEs), have been developed in human cells. However, despite their importance crop genome editing, the efficacy CGBEs has not yet extensively evaluated. In our study, based on previously reported plant-compatible CBE CGBE, we demonstrated monocot (PcCGBEs) (C-to-G) rice protoplasts. For all targets tested, PcCGBEs (monocot CGBEs) appeared to...
Antiangiogenic therapies targeting VEGFA have been commonly used in clinics to treat cancers over the past decade. However, their clinical efficacy has limited, with drawbacks including acquisition of resistance and activation compensatory pathways resulting from elevated circulating VEGFB placental growth factor (PlGF). To bypass these disadvantages, we developed a novel glycosylated soluble decoy receptor fusion protein, VEGF-Grab, that can neutralize VEGFA, VEGFB, PlGF. VEGF-Grab second...
Interleukin-20 (IL-20) is a member of the IL-10 family. IL-20 expression regulated by transcription elongation factor, Ell3, in estrogen receptor-positive (ER(+)) breast cancer cells. In this study, we demonstrated that ER(α), GATA3 and FOXA1 form transcriptional complex with Ell3 to regulate ER(+) We also determined share binding site ER(α) interleukin-20 promoter. Furthermore, found represses expression, whereas activate it. addition, associates increase its affinity promoter, which may...
While adipose-derived stem cell-conditioned medium (ADSC-CM) has been demonstrated to promote skin wound healing, the mechanism regulating this effect remains unelucidated. In study, we aimed investigate role of Ell3 in healing activity ADSC-CM. vitro analysis revealed that suppression ADSCs impairs promotive ADSC-CM on proliferation and migration mouse embryonic fibroblasts (MEF) normal human dermal (NHDF). Consistently, expression MMP family genes, which regulate cell migration, was...
Macrophages have been associated with drug response and resistance in diverse settings, thus raising the possibility of using macrophage imaging as a companion diagnostic to inform personalized patient treatment strategies.Nanoparticle-based contrast agents are especially promising because they efficiently deliver fluorescent, magnetic, and/or radionuclide labels by leveraging intrinsic capacity macrophages accumulate nanomaterials their role professional phagocytes.Unfortunately, current...
The efficient nonviral delivery of nucleic acids into the cytoplasm is needed to fully realize potential gene therapy. Although cationic lipids and nanoparticles have been widely used improve intracellular acids, they suffer from cytotoxicity poor endosomal escape, thus limiting transfection efficacy. Here, we developed a photothermal platform for biosafe acids. Photothermal was carried out by irradiation cells co-treated with Lipofectamine-plasmid DNA complexes PEGylated gold nanorods...
Many studies have been conducted on the transduction efficiency of recombinant adeno-associated virus (rAAV) depending serotype and genome structure, such as single-stranded (ss) self-complementary (sc). To understand variation in therapeutic efficacy, we focused investigating subcellular distribution viral rAAV structure. It is critical to ascertain location within host cell after entry because a larger amount placed nucleus facilitates replication by utilizing cell's system, thereby...
Abstract In this study, oil‐in‐water nanoemulsions are prepared, an isotropic mixture of oil, surfactant, and cosurfactants. The exhibit stable structures capable efficiently encapsulating hydrophobic drugs such as doxorubicin (Dox). Compared to polymeric micelles, demonstrate enhanced stability loading capacity for Dox. Furthermore, release Dox steadily over 14 days, with 51.6% released within the initial 24 h up 80% subsequent period. These properties suggest that can mitigate side effects...
Abstract Long noncoding RNAs (lncRNAs) act as transcriptional regulators in plants and animals. To date, they have been reported to regulate various biological processes, such phosphate homeostasis, grain yield, fertility rice ( Oryza sativa L.). However, the lncRNAs involved abiotic stress responses remain poorly identified rice. In this study, we analyzed expression profiles of using public transcriptome datasets derived from stress-treated samples. We found that thousands was...