A5025789491- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Genetic diversity and population structure
- Plant pathogens and resistance mechanisms
- Infections and bacterial resistance
- Chromosomal and Genetic Variations
- Atmospheric chemistry and aerosols
- Plant responses to elevated CO2
- Plant Pathogens and Resistance
- Machine Learning in Bioinformatics
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Wheat and Barley Genetics and Pathology
- Vibrio bacteria research studies
Max Planck Institute for Biology
2022-2024
University of Toronto
2007-2022
Massey University
2013-2021
Max Planck Institute for Developmental Biology
2020-2021
South China Botanical Garden
2016-2017
Chinese Academy of Sciences
2016-2017
Beijing Botanical Garden
2017
Allan Wilson Centre
2013
The origins of crop diseases are linked to domestication plants. Most crops were domesticated centuries – even millennia ago, thus limiting opportunity understand the concomitant emergence disease. Kiwifruit (Actinidia spp.) is an exception: began in 1930s with outbreaks canker disease caused by P. syringae pv. actinidiae (Psa) first recorded 1980s. Based on SNP analyses two circularized and 34 draft genomes, we show that Psa comprised distinct clades exhibiting negligible within-clade...
Horizontal gene transfer can precipitate rapid evolutionary change. In 2010 the global pandemic of kiwifruit canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) reached New Zealand. At time introduction, single clone responsible for outbreak was sensitive to copper, however, analysis a sample isolates taken in 2015 and 2016 showed that quarter were copper resistant. Genome sequences seven strains resistance - comprising czc/cusABC copABCD systems along with arsenic cadmium,...
Recurring epidemics of kiwifruit (Actinidia spp.) bleeding canker disease are caused by Pseudomonas syringae pv. actinidiae (Psa). In order to strengthen understanding population structure, phylogeography, and evolutionary dynamics, we isolated from cultivated wild across six provinces in China. Based on the analysis 80 sequenced Psa genomes, show that China is origin pandemic lineage but strain diversity confined just a single clade. contrast, Korea Japan harbor strains multiple clades....
The innate immune system is an ancient and broad-spectrum defense found in all eukaryotes. detection of microbial elicitors results the up-regulation defense-related genes elicitation inflammatory apoptotic responses. These responses are front-line barrier against disease because they collectively suppress growth vast majority invading microbes. Despite their critical role, we know remarkably little about diversity elicitors. To address this paucity, reasoned that hosts more likely to evolve...
Summary Reticulate speciation caused by interspecific hybridization is now recognized as an important mechanism in the creation of biological diversity. However, depicting patterns phylogenetic networks for lineages that have undergone gene flow challenging. Here we sequenced 25 taxa representing natural diversity genus Actinidia with average mapping depth 26× on reference genome to reconstruct their reticulate history. We found evidence, including significant tree discordance, cytonuclear...
Integrative and conjugative elements (ICEs) are self-transmissible mobile that transfer functional genetic units across broad phylogenetic distances. Accessory genes shuttled by ICEs can make significant contributions to bacterial fitness. Most characterized date encode readily observable phenotypes contributing symbiosis, pathogenicity, antimicrobial resistance, yet the majority of carry unknown function. Recent observations rapid acquisition in a pandemic lineage Pseudomonas syringae pv....
Wild plant species are threatened by diverse pathogens, but disease symptoms rarely observed in nature. This suggests that wild plants harbor valuable sources of resistance. In this study, we show a model bacterial pathogen Pseudomonas syringae pv. tomato (Pto) DC3000 triggered defense responses all tested accessions Solanaceae species, Solanum americanum. Pto DC3000-triggered immunity S. americanum required type III secretion system. We seven effectors (AvrPto, HopAD1, HopAM1, HopC1,...
Summary Whole‐genome annotation error that omits essential protein‐coding genes hinders further research. We developed Target Gene Family Finder ( TGFam‐Finder ), an alternative tool for the structural of containing target domain(s) interest in plant genomes. took considerably reduced run‐time and improved accuracy compared to conventional tools. Large‐scale re‐annotation 50 genomes identified average 150, 166 86 additional far‐red‐impaired response 1, nucleotide‐binding leucine‐rich‐repeat,...
Summary Interactions between commensal microbes and invading pathogens are understudied, despite their likely effects on pathogen population structure infection processes. We describe the genetic diversity of a broad range co‐occurring Pseudomonas syringae isolated from infected uninfected kiwifruit during an outbreak bleeding canker disease caused by P. pv. actinidiae ( Psa ) in New Zealand. Overall was clonal affected ecological factors including status cultivar. Most isolates members new...
Summary The common polysaccharide antigen (CPA) of the lipopolysaccharide (LPS) from Pseudomonas syringae is highly variable, but genetic basis for this poorly understood. We have characterized CPA locus P. pv. actinidiae ( Psa ). This has genes l ‐ and d ‐rhamnose biosynthesis an operon coding ABC transporter subunits, a bifunctional glycosyltransferase o ‐methyltransferase. predicted to role in transport, elongation termination oligosaccharide referred as TET operon. Two alleles were...
Abstract Ralstonia solanacearum causes bacterial wilt disease in many plant species. Type III‐secreted effectors (T3Es) play crucial roles pathogenesis. However, some T3Es are recognized by corresponding resistance proteins and activate immunity. In this study, we identified the R. T3E protein RipAZ1 ( injected AZ1) as an avirulence determinant black nightshade species Solanum americanum . Based on S. accession‐specific phenotype of strain Pe_26, 12 candidate were selected for further...
Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) threatens cultivation of important crops worldwide. We sequenced 30 RSSC phylotype I ( R. pseudosolanacearum ) strains isolated from pepper Capsicum annuum and tomato Solanum lycopersicum across Republic Korea. These isolates span diversity I, have extensive effector repertoires are subject to frequent recombination. Recombination hotspots among South Korean include multiple predicted contact-dependent inhibition...
Ralstonia solanacearum causes bacterial wilt disease in solanaceous crops. Identification of avirulence type III-secreted effectors recognized by specific resistance proteins host plant species is an important step toward developing durable In the present study, we show that R. effector RipJ functions as determinant Solanum pimpinellifolium LA2093. all, 10 candidate were shortlisted based on repertoire comparison between avirulent Pe_9 and virulent Pe_1 strains. Infection assays with...
Lateral gene transfer can precipitate rapid evolutionary change. In 2010 the global pandemic of kiwifruit canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) reached New Zealand. At time introduction, single clone responsible for outbreak was sensitive to copper, however, analysis a sample isolates taken in 2015 and 2016 showed that quarter were copper resistant. Genome sequences seven strains resistance (comprising czc/cusABC copABCD systems) along with arsenic cadmium,...
Abstract Background and Aims Low atmospheric CO2 concentration depresses photosynthesis resource use efficiency, therefore can inhibit phases of the life cycle such as seedling establishment. Seed reserves compensate for photosynthetic inhibition by accelerating growth. We hypothesize that seedlings arising from large seeds show less low than young plants small seeds. size effects on responses to may also be enhanced in warm environments, due greater photorespiration at high temperature....
A strain of Pseudomonas syringae pv. actinidiae (Psa) that is particularly virulent (Psa-V or biovar 3) responsible for the recent devastating global outbreak bacterial canker on kiwifruit vines. We have now sequenced genomes over 30 isolates Psa from around world including two and type strain) to near completion. This extensive resource genomic information has allowed us reveal remarkable plasticity exhibited by this pathogen trace its evolution first geographically limited outbreaks...
ABSTRACT Recurring epidemics of kiwifruit ( Actinidia spp.) bleeding canker disease are caused by Pseudomonas syringae pv. actinidiae Psa ), whose emergence coincided with domestication its host. The most recent pandemic has had a deleterious effect on production worldwide. In order to strengthen understanding population structure, phylogeography and evolutionary dynamics , we sampled 746 isolates from cultivated wild across six provinces in China, which 87 were . Of 234 isolated spp. none...