The plant growth-repressing DELLA proteins (DELLAs) are known to represent a convergence point in integration of multiple developmental and environmental signals planta, one which is hormone gibberellic acid (GA). Binding the liganded GA receptor (GID1/GA) N-terminal domain DELLAs required for GA-induced degradation via ubiquitin-proteasome pathway, thus derepressing growth. However, conformational changes upon binding GID1/GA, key understanding precise mechanism GID1/GA-mediated DELLAs,...

Summary The common polysaccharide antigen (CPA) of the lipopolysaccharide (LPS) from Pseudomonas syringae is highly variable, but genetic basis for this poorly understood. We have characterized CPA locus P. pv. actinidiae ( Psa ). This has genes l ‐ and d ‐rhamnose biosynthesis an operon coding ABC transporter subunits, a bifunctional glycosyltransferase o ‐methyltransferase. predicted to role in transport, elongation termination oligosaccharide referred as TET operon. Two alleles were...

The phytohormone gibberellin and the DELLA proteins act together to control key aspects of plant development. Gibberellin induces degradation by recruitment an F-box protein using a molecular switch: gibberellin-bound nuclear receptor interacts with N-terminal domain proteins, this event primes C-terminal for interaction protein. However, mechanism signalling between N- domains is unresolved. In present study, we used in vivo vitro approaches characterize di- tri-partite interactions RGL1...

The purpose of this study was to assess the degree which teachers' expectations are related children's social class characteristics. A sample 96 elementary school teachers drawn from four schools serving lower- and middle-upper neighborhoods asked judge performance potential characteristics, including SES background, a set photographs both black white grade children. While were found be positively significantly perceived children in photos, variations by number years teaching experience mean...

Four generic heterobifunctional reagents, namely 2-(2-chloro-5-methyl-1,3,2-dioxaphosphorinan-5-yl)methoxyacetic acid methyl ester, p-sulfide, p-oxide, 2-(2-mercapto-5-methyl-1,3,2-dioxaphosphorinan-5-yl)methoxyacetic bispotassium salt, p-sulfide-, and (2-methoxy-5-methyl-1,3,2-dioxaphosphorinan-5-yl)methoxyacetic acid, have been synthesized used to prepare organophosphate, thiophosphate, dithiophosphate haptens containing a functional carboxyl group which can be conjugate the proteins....

2‐(2‐Mercapto‐5‐methyl‐1,3,2‐dioxaphosphorinan‐5‐yl,2‐sulphide)methoxyacetic acid has been synthesized and used to prepare an azinphos hapten protein conjugates. Monoclonal antibodies of high affinity against the pesticide azinphos‐methyl were prepared from mice immunized with hapten‐ovalbumin conjugate. The best monoclonal antibody could be in indirect enzyme‐linked immunosorbent assay (ELISA) quantify at concentrations 50 pg ml‐1 2 ng (2.5–100 pg/assay). corresponding limit detection was...

Antibodies to Gardnerella vaginalis were raised in rabbits. Nine antisera that reacted with their immunising strains, but not the remaining eight used develop a serotyping scheme. A dot blotting technique was used, and complexes of antigen antibody visualised using anti-rabbit immunoglobulin linked alkaline phosphatase. Of 91 clinical isolates evaluate scheme, 79 (87%) typable 52 (57%) only single antiserum. The antigens expressed stable during growth on different media subculture....

Abstract Coronatine (COR) is composed of two structural components, coronafacic acid (CFA) and the amino coronamic (CMA), linked by an amide bond. Monoclonal antibodies (MAbs) were prepared against COR incorporated into competitive ELISAs. MAbs secreted hybridoma cells which had been from mice immunized with conjugated, through free carboxyl group on CMA, to ovalbumin, available groups increased derivatization propyl diamine via groups. coronafacoyl valine could be quantified at 6.800 ng...

Dothistromin (DOTH) is a fungal toxin occurring in Pinus radiata needles contaminated with Dothistroma pini. Monoclonal antibodies (MAbs) of high affinity were prepared against DOTH and incorporated into competitive ELISAs. MAbs secreted by hybri‐doma from mice immunized conjugated, through the aromatic ring anthraquinone, to bovine serum albumin. could be quantitated at 2–60 ng ml‐1 (0.1–2 ng/assay) 8–300 (0.4–15 using peroxidase‐labelled MAb 10C12A5 6A2D4 respectively indirect ELISA. The...

Abstract Coronatine (COR) is composed of two structural components, coronafacic acid (CFA) and the amino coronamic (CMA), which are joined by an amide bond. Monoclonal antibodies (MAbs) were prepared against COR used in competitive ELISAs. MAbs secreted hybridoma cell lines that from mice immunized with different analogues conjugated to ovalbumin (OVA). The first hapten was reduction keto-oxygen on cyclopentane ring CFA moiety subsequent reaction hydroxyl group succinic or glutaric...

Dothistromin (DOTH) is a toxin secreted by Dothistroma pini and present in needle lesions resulting from infection this fungus. A monoclonal antibody (MAb 10C12) of high affinity for DOTH was conjugated to bovine serum albumin (BSA) used immunize mice order prepare anti‐idiotypic mimics dothistromin. One antibody, 12C9G8, equivalent on molar basis inhibition the binding an anti‐DOTH MAb DOTH‐protein conjugates. Furthermore, Western blot analysis, 12C9G8 visualized same protein profile...

Six hybridoma clones were obtained that secreted monoclonal antibodies against the aspartate aminotransferase-P1 (AAT-P1) isoenzyme from root nodules of Lupinus angustifolius [L.] cv Uniharvest. This enzyme is found constitutively in plant cytosol fraction. The produced all immunoglobulin G1 class, recognized two distinct epitopes on protein, and represented major paratopes fraction sera taken mice rabbits immunized with pure AAT-P1 protein. One these was unique to lupin nodule AAT-P1. other...

The phytopathogenic fungus Venturia inaequalis causes scab of apple. Once this penetrates the plant surface, it forms a specialized body called stroma between inner cuticle surface and epidermal cell wall. A novel 5704 (Cin3) three expressed sequence tags (ESTs); 38, 6987, 4010 are strongly up-regulated in early stages infection. CIN3 ESTs using two vectors pMAL-c2 pET 21 were Escherichia coli. Recombinant proteins expression, solubility yields analyzed. 6987 re- combinant soluble form while...

A trans-acting protein interacting with a specific sequence motif proximal to the transcriptional start site of L-asparaginase promoter has been observed previously (E. Vincze, J. M. Reeves, E. Lamping, K. F. Farnden, and P. H. S. Reynolds, Plant Mol. Biol. 26:303-311, 1994). Gel retardation experiments in which extracts Mesorhizobium loti developing nodules were used suggested bacterial origin for repressor binding (rep2037). Nodulation tests performed by using different Fix(-) Tn5 mutants...

The identification of clones expressing high levels recombinant protein in Pichia pastoris is usually dependent upon SDS-PAGE, Western blotting, or bioactivity-based assays that are labor and time-consuming. We describe a rapid method images green fluorescence (GFP) individual P. transformed with vectors express the proteins as GFP C- terminal fusion. In this report we have used system to monitor expression three from Venturia inaequalis. Culture plates containing colonies were imaged on...

Coronatine (COR) is composed of two structural components, coronafacic acid (CFA) and the amino coronamic (CMA), which are joined by an amide bond. Monoclonal anti-idiotypic antibodies were prepared used in competitive ELISAs. (MAbs) secreted hybridoma cell lines from mice immunized with different idiotypic MAbs, 11B8 11G8 that predominantly recognized CMA or CFA residues, respectively. Hybridoma secreting high affinity MAbs 4D5 (anti 11B8) 4H10 11G8) isolated. MAb mimicked coronamyl...

<h3>Background</h3> Following a presentation to therapy colleagues on the benefits of horticultural (HT) for clients with range health problems it was felt that attending our day hospice may benefit from similar project. During conversations attendees found several had voiced their sadness they were no longer well enough tend own gardens and something dearly missed. meetings colleagues, team agreed pilot six- month HT group. Two small successful funding applications allowed us start in...

Monoclonal antibodies (MAbs) were generated against recombinant human insulin-like growth factor 1 (IGF-1) by fusion of NS-1 myeloma cells with spleen from BALB/c X DBA mice immunised IGF-1 and synthetic peptide sequences derived the published amino acid sequence IGF-1. MAbs produced that recognised four distinct epitopes including two defined segments C D domains. All IgM mouse immunoglobulins. These results indicate feasibility producing to highly conserved proteins.

Abstract: A single chain antibody mimic (MScFvlOE7) was constructed, using a phage display recombinant system, from RNA isolated the splenocytes of mice immunised with monoclonal MAblOC12 which recognises furan ring DOTH [l]. MScFvlOE7 competed for binding to in two ELISA formats and .also reacted protein [2] extracted mature Pinus radiata embryos is -type anti-idiotypic DOTH.

ABSTRACT The common polysaccharide antigen (CPA) from the lipopolysaccharide (LPS) component of cell walls species complex Pseudomonas syringae is highly variable both in structure and immunological specificity, but genetic basis for this not well understood. We have characterised CPA locus P. pv. actinidiae ( Psa ). This has a modular with genes L- D- rhamnose (Rha) biosynthesis that an unknown sugar. It also contains operon coding ABC transporter subunits, bifunctional glycosyltransferase...